Anti-FADD Antibody

Product Name

Anti-FADD Antibody

View all FADD Antibodies

SKU/Catalog Number

PA1464

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FADD Antibody catalog # PA1464. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FADD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1464)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human FADD.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1464 is reactive to FADD in Human, Mouse, Rat

Applications

PA1464 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

23.279kDa

Background of FADD

FADD, Fas-Associated protein with Death Domain, is a universal adaptor protein in apoptosis that mediates signaling of all known death domain-containing members of the TNF receptor superfamily. The FADD gene contains 2 exons and spans approximately 3.6 kb. By analysis of somatic cell hybrid panels and by fluorescence in situ hybridization, the FADD gene is mapped to 11q13.3. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, thus, it participates in the death signaling initiated by these receptors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of FADD using anti-FADD antibody (PA1464).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT1080 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: human MCF-7 whole cell lysates,
Lane 8: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # PA1464) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADD at approximately 28 kDa. The expected band size for FADD is at 23 kDa.

Click image to see more details

Figure 2. Western blot analysis of FADD using anti-FADD antibody (PA1464).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # PA1464) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADD at approximately 28 kDa. The expected band size for FADD is at 23 kDa.

Gene/Protein Information For FADD (Source: Uniprot.org, NCBI)

Alternative Names

FADD; Fas (TNFRSF6)-associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; GIG3; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; mediator of receptor-induced toxicity; MGC8528; MORT1; MORT1Fas-associating protein with death domain; protein FADD FADD GIG3, MORT1 Fas associated via death domain FAS-associated death domain protein|Fas (TNFRSF6)-associated via death domain|Fas-associating death domain-containing protein|Fas-associating protein with death domain|growth-inhibiting gene 3 protein|mediator of receptor-induced toxicity

For more info on FADD, check out the FADD Infographic

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In this infographic, you will see the following information for FADD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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