Glutamate decarboxylase 1 Antibodies

Glutamate decarboxylase 1 (GAD1)

Catalyzes the production of GABA.

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Gene Information

Human
Gene Name:	GAD1
Uniprot:	Q99259
Entrez:	2571

Family

Belongs to:
group II decarboxylase family

Alternative Names

67kD); EC 4.1.1; FLJ45882; GAD1; GAD25; GAD67; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform

Molecular Weight

Mass (kDA):
66.897 kDA

Genomic Context

Human
Location:	2q31.1
Sequence:	2; NC_000002.12 (170813210..170861151)

Tissue Specificity

Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

Diseases

• Schizophrenia
• Bipolar Disorder
• Depressive Disorder
• Nervousness
• Dysequilibrium Syndrome
• Autistic Disorder
• Anxiety Disorders
• Mental Disorders
• Psychotic Disorders
• Epilepsy
• Mood Disorders

• Hypoxia
• Cleft Palate
• Major Depressive Disorder
• Nerve Degeneration
• Substance Withdrawal Syndrome

Interacting Proteins

• AGTRAP
• CMTM5
• HAAO
• MAL2
• NCK1

• STK3

Pathways

• Methylation
• Dna Methylation
• Pathogenesis
• Brain Development
• Chromatin Remodeling
• Aging
• Neurogenesis
• Transport
• Palate Development
• Synaptic Transmission
• Neuron Differentiation
• Localization
• Cell Adhesion

• Response To Heat
• Reflex
• Cognition
• Cell Death
• Locomotion
• Gabaergic Neuron Differentiation
• Dna Demethylation

PTMs

• Methylation
• Acetylation

• Demethylation
• Glycosylation

• Decarboxylation

Research Areas

• Diabetes Research

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GAD1

Best Uses Of The GAD1 Marker

This article will discuss the benefits of using the GAD1 Marker, its applications in cell-based ELISA kits, and how to validate Boster Bio ELISA kits. These benefits are applicable to scientists around the world. Read on to learn more. This article also explains the differences between a standard ELISA and a Boster Bio ELISA kit. Using the Boster ELISA is more convenient and accurate than other standard ELISA kits.

Benefits of using the GAD1 Marker

Currently, studies have demonstrated the value of the GAD1 marker for assessing the presence of GABA-producing neurons. This protein is found in synaptic vesicles of nerve endings and is a key mediator of GABA synthesis. A series of studies have shown increased post-synaptic inhibitory potentials in Hippocampal neurons and decreased inhibitory currents in Purkinje cells in rat cerebellar slices treated with GAD-positive serum. In addition, passive transfer studies have revealed increased excitability of anterior horn cells and continuous motor activity.

Applications of the GAD1 Marker in research

The GAD1 marker is useful in several studies, as it can be used to investigate changes in GABAergic transmission. In particular, it can be used to study changes in GABA levels in the prefrontal cortex and interneurons. However, further studies are required in larger samples of Han Chinese subjects. Further, studies on patient cells will be necessary in order to determine whether the GAD1 marker has a role in the disease process.

The gene encoding GAD is composed of 16 exons, which are separated by a common 5-prime untranslated region. The GAD1 gene has one exon on human chromosome 2 while its corresponding protein is located on mouse chromosome 2D. The cDNA clones were obtained from fetal brain and frontal cortex. These cDNA clones contain a single exon and a partial exon. The gene encodes a protein with 594 amino acids and a molecular weight of 67 kD. However, the GAD1 gene is also present on chromosome 10 and a pseudogene. In addition, the GAD1 marker has been used in several studies to detect inherited disorders, such as Alzheimer's disease.

The GAD1 gene was first identified by Sparkes, R. S. and Kaufman, D. L. in 1987. Using somatic cell hybrid analysis, they assigned GAD1 to human chromosome 2 and identified a corresponding gene on chromosome 2. However, these studies failed to detect a strong association between GAD1 and schizophrenia. The results were mixed and inconsistent.

Currently, GAD1 is expressed in the pancreas. GAD1 encodes the 67 kDa isoform of glutamic acid decarboxylase. It is present in both the pancreatic islets and the CNS. Research suggests that GAD67 plays a role in neuronal development and is involved in a variety of skin activities. It is part of a group II decarboxylase family.

Furthermore, the GAD1 gene encodes GAD65 and NPY/AgRP neurons. Fasting increased the expression of Gad1, which in turn increases the likelihood of GABA release. In addition, selective inhibition of GAD activity decreases the release of GABA from NPY/AgRP neurons. To find the GAD1 gene, researchers need to know whether it regulates the release of GABA.

Application of the GAD1 Marker in cell-based ELISA kits

In-cell ELISA, also known as in-cell western blot, is a method of measuring protein levels within cells. It is an effective method for screening the effects of drugs and therapies that change the expression levels of target proteins. This assay kit is designed to measure relative amounts of GAD1 in cells. This is a highly sensitive and specific test for detecting cellular GAD1 protein levels.

GAD1 is an enzyme that encodes the 67-kDa glutamate decarboxylase isoform. This is the rate-limiting enzyme in the biosynthesis of GABA and is highly expressed in early human brain development. It also plays an important role in skin activity. GAD1 is part of the family of group II decarboxylases. It has been shown to promote cognitive function recovery after cerebral ischemia, improve afferent nerves and facilitate growth in mice.

The cellular seeding density of the assay microplate must be determined. It depends on the cell type, size, and abundance of the target protein. It is advisable to use monolayers when possible to generate a robust signal. To determine the appropriate cell seeding density, you may serially dilute adherent cells and observe them under the microscope. High seeding densities will generate a stronger signal, while working at the higher end of the scale will allow you to detect even the smallest signal increases.

Application of the GAD1 Marker in cells-based ELISA kits involves the detection of a specific protein, glutamate decarboxylase. This enzyme synthesizes the essential neurotransmitter GABA from L-glutamic acid. Both GAD1 and GAD2 are encoded by separate genes, but they are coexpressed in most GABA-containing neurons.

Cellular sections of the crista are stained with DAPI and are reconstructed to show the topographical distribution of supporting cells. In addition to GAD1, GAD67 is also expressed in the peripheral zone of the crista. In contrast, cells at the central zone are not stained, and the E.C. is lacking in GAD67. These results are consistent with previous experiments.

Validation of Boster Bio ELISA kits

To ensure accurate results, the manufacturer of Boster Bio ELISA kits validates them against relevant superfamilies or immunogenic proteins. These antibodies are highly specific and can detect native forms of proteins. Boster QC tests all ELISA kits to ensure consistency from lot to lot. For more information, visit the company website. ELISA kits are the most common type of immunoassays in research laboratories, and a QC report can provide details on the kit's quality and performance.

The CV% variation for a particular protein assay was measured. These values were obtained from internal QC results, which may not match end user lab test results. The CV% is more important than the exact O.D. value, as this is indicative of linearity. Boster bio validates its assays by generating dilution series across the dynamic range of the assay.

Boster Bio is an antibody manufacturer and developer. They specialize in high-specificity, picogram-sensitive ELISA kits, and WB/IHC compatible antibodies. Their antibodies are thoroughly validated and have been published in over 29,000 scientific publications. The antibodies are tested against 250 tissue samples and untransfected cell lines. They are then evaluated in ELISA, WB, and Flow Cytometry applications.

ELISA protocols start with sample preparation. Depending on the protein you want to detect, different samples may require specialized treatment. Boster's troubleshooting guides help you eliminate most sources of error. For additional guidance, check out the company's technical blog. These articles provide in-depth information on ELISA applications and contain tips and tricks for optimizing your experiments. Whether you're a newbie or have been using ELISA kits for a while, you can benefit from these technical articles and guides.