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- Table of Contents
Designing a Western blot for Glutamate decarboxylase 1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the GAD1 Western Blot Guide5 Citations
Facts about Glutamate decarboxylase 1.
Catalyzes the production of GABA.
.| Human | |
|---|---|
| Gene Name: | GAD1 |
| Uniprot: | Q99259 |
| Entrez: | 2571 |

| Belongs to: |
|---|
| group II decarboxylase family |

67kD); EC 4.1.1; FLJ45882; GAD1; GAD25; GAD67; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform
Mass (kDA):
66.897 kDA

| Human | |
|---|---|
| Location: | 2q31.1 |
| Sequence: | 2; NC_000002.12 (170813210..170861151) |
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.





PMID: 1549570 by Bu D.-F., et al. Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene.
PMID: 8088791 by Bu D.-F., et al. The exon-intron organization of the genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD67 and GAD65) suggests that they derive from a common ancestral GAD.
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