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HSPA5 / Endoplasmic reticulum chaperone BiP · IHC design guide
Design Immunohistochemistry for HSPA5
This guide plans chromogenic HSPA5 IHC around cytoplasmic staining, with duodenal enterocytes as a high-staining reference (HPA tissue IHC). It covers fixation consistency, control selection and scoring against broadly distributed tissue staining (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
HSPA5 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic staining in tissue IHC (HPA tissue IHC) | |
| Staining pattern | Cytoplasmic; high in immune, neuronal and thyroid follicular cells (HPA tissue IHC) | |
| Antigen retrieval | Citrate pH 6.0 HIER, 95–98 °C, 20 min (rule: cytoplasmic / membrane antigen) | |
| Positive control | Caudate+4 more · see all | |
| Negative control | None in HPA (detected in all 45 tissues); use no-primary + isotype controls |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image M00955) | |
| Caveat | Ubiquitous expression limits negative tissue controls (HPA tissue IHC) | |
| Regulation | Expression regulation is unreported (UniProt) | |
| Isoform / epitope | No isoforms annotated; mature chain spans aa 19–654 (UniProt) |
Section 1
Recommended HSPA5 IHC & IF Protocols
Compare the catalog antibody’s IHC-P protocol with published HSPA5 staining methods for placenta (PMC9926443) and glioma tissue (PMC8121073).
| Sample | Paraffin-embedded tissue sections; fixative not specified (datasheet M00955; sample unspecified) |
| Fixation | Image fixative and duration unreported (datasheet M00955); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat-induced epitope retrieval in citrate buffer, pH 6.0, 20 min at 95–98 °C (standard rule: cytoplasmic / membrane antigen) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% normal serum of the secondary host, 30 min, room temperature (standard) |
| Primary antibody | Rabbit monoclonal (clone BOO-8) anti-HSPA5, 1:50-1:200 (datasheet M00955) |
| Primary incubation | Overnight at 4 °C (standard) |
| Detection | HRP-polymer secondary, DAB chromogen 5–10 min (standard) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | HSPA5-positive staining in neuronal cells of caudate (HPA tissue IHC: High). HPA tissue profile: Ubiquitous cytoplasmic expression, highly abundant in immune, neuronal cells and thyroid follicular cells. No signal in the no-primary control. |
💡Decision noteStart with citrate HIER at pH 6.0, 95–98 °C for 20 min (page antigen retrieval); the glioma excerpt does not specify retrieval conditions (PMC8121073).
Section 2
What Is the Expected HSPA5 Staining Pattern?
HSPA5 is primarily an ER-lumen chaperone with no transmembrane segment (UniProt P11021 topology). In paraffin-section IHC, expect cytoplasmic staining across many tissues, with prominent signal in immune cells, neuronal cells and thyroid follicular cells (HPA tissue IHC: ubiquitous cytoplasmic expression). HPA rates the tissue staining reliability Enhanced, while reporting medium consistency between staining and RNA expression (HPA tissue IHC: reliability).
| Cytoplasmic staining in neuronal cells or duodenal enterocytes. | This fits an ER-associated cytoplasmic pattern (UniProt P11021: ER lumen; HPA tissue IHC: cytoplasmic expression). Neuronal cells in caudate and cerebral cortex, and duodenal enterocytes, are recorded as High (HPA tissue IHC: High). Assess the cell type and distribution alongside intensity; a strong brown deposit alone does not establish specificity (standard IHC practice). |
| Nuclear-only staining dominates, with little cytoplasmic signal. | This conflicts with the expected tissue IHC pattern (HPA tissue IHC: ubiquitous cytoplasmic expression). Treat it as suspect and check control sections, antibody conditions and counterstain interpretation (standard IHC practice). Do not automatically reject all non-ER signal: UniProt also lists cytoplasm and reports cell-surface localization under high free-iron conditions (UniProt P11021: subcellular location). |
| The strongest signal appears in a cell population outside the expected pattern. | Check cell identity before calling the result specific. HPA records High staining in lung alveolar type II cells but Low staining in oral-mucosa squamous epithelial cells (HPA tissue IHC: High; Low). Unexpected staining can reflect antibody cross-reactivity or endogenous detection activity; compare reagent controls and tissue morphology (standard IHC practice). Low is not a validated negative (HPA tissue IHC: Low; negative list empty). |
| Brown deposit spreads across cells, stroma or section edges without a clear cellular pattern. | This is diffuse background rather than a convincing cytoplasmic result (HPA tissue IHC: cytoplasmic expression; standard IHC practice). Review the secondary-only control, blocking, washes and detection exposure to locate nonspecific or endogenous signal (standard IHC practice). Avoid scoring background intensity as HSPA5 expression (standard IHC practice). |
| A known-positive tissue has no interpretable cellular signal. | An absent signal in a High reference population, such as endometrial glandular cells or lung alveolar type II cells, calls the staining run into question (HPA tissue IHC: High). Check section integrity, reagent controls and detection performance before interpreting study samples (standard IHC practice). HPA's Enhanced IHC rating supports the reported pattern but does not guarantee every run (HPA antibodies: IHC Enhanced). |
💡Expected HSPA5 appearanceCall a positive result when clear cytoplasmic staining is present in an HPA High cell population, such as duodenal enterocytes or cortical neurons; nuclear-only deposit or uniform tissue-wide haze is suspect (HPA tissue IHC: cytoplasmic expression; High; standard IHC practice).
| Cellular compartment and topology | ER-lumen localization explains a cytoplasmic IHC readout in sectioned cells (UniProt P11021: ER lumen; HPA tissue IHC: cytoplasmic expression). HSPA5 has no transmembrane segment, yet conditional cell-surface localization is reported in epithelial cells exposed to high free iron; surface staining alone therefore needs context and controls (UniProt P11021: topology; subcellular location). |
| Tissue reference and validation | HPA reports High staining in several specific cell populations and Low staining in others, with no tissue in its supplied negative list (HPA tissue IHC: High; Low; negative list empty). Its tissue result is Enhanced with medium staining-versus-RNA consistency, and three listed antibodies have Enhanced IHC validation (HPA tissue IHC: reliability; HPA antibodies: IHC Enhanced). Use Low tissue as a contrast, not a zero-signal control (HPA tissue IHC: Low). |
| Protein processing and epitope interpretation | UniProt annotates a signal peptide at residues 1–18 and a mature chain at 19–654, with no propeptide or transmembrane segment (UniProt P11021: processing; topology). The supplied record does not locate this antibody's epitope, so it cannot predict epitope exposure, antigen-retrieval needs or fixation sensitivity for HSPA5 (UniProt P11021: processing; supplied evidence scope). |
| IF/ICC Q: Should IF show the same pattern as tissue IHC? | A: Do not equate the two readouts. Tissue IHC is described as ubiquitously cytoplasmic (HPA tissue IHC: profile). HPA ICC-IF lists cytosol as uncertain, while annulus and sperm mid, principal and end-piece locations are approved (HPA subcellular ICC-IF: location status). Interpret IF/ICC with its own controls and guide (standard IF practice). |
| Situation | Likely cause | Next action |
|---|---|---|
| High-reference cells are blank while the counterstain is visible. | The staining or detection run may have failed; an HPA High label does not establish what failed in this section (HPA tissue IHC: High; standard IHC practice). | Confirm the reference cell type on the section, then inspect primary-antibody application and detection controls. Review antigen retrieval as a general paraffin-IHC variable without assuming HSPA5-specific fixation sensitivity (standard IHC practice). |
| Nuclei dominate the signal in an otherwise intact section. | That distribution conflicts with HPA's cytoplasmic tissue pattern; nuclear deposit may be nonspecific or reflect interpretation of the counterstain (HPA tissue IHC: cytoplasmic expression; standard IHC practice). | Compare the primary-omission or secondary-only control and inspect a known High cell population for cytoplasmic signal before scoring the section (HPA tissue IHC: High; standard IHC practice). |
| Low-reference squamous cells stain as strongly as the chosen High-reference cells. | The contrast differs from the supplied HPA levels, although Low does not mean absent (HPA tissue IHC: oral-mucosa squamous cells Low; duodenal enterocytes High). | Recheck cell identity and use matched imaging or scoring conditions. Inspect reagent controls for nonspecific deposit before concluding that the staining is HSPA5-specific (standard IHC practice). |
| Diffuse brown haze obscures cell boundaries. | Nonspecific background or endogenous detection activity can obscure the expected cellular distribution (HPA tissue IHC: cytoplasmic expression; standard IHC practice). | Inspect primary-omission and detection controls; adjust blocking, washing or detection exposure according to the control result (standard IHC practice). Score only interpretable cellular staining (standard IHC practice). |
| Signal is seen mainly at epithelial cell surfaces. | UniProt reports conditional surface localization with high free iron, but the tissue IHC profile is broadly cytoplasmic (UniProt P11021: subcellular location; HPA tissue IHC: profile). | Record the compartment separately from cytoplasmic staining, inspect controls and avoid inferring iron exposure from the image alone (standard IHC interpretation). |
| An IF/ICC image and the paraffin IHC slide appear to disagree. | HPA labels ICC-IF cytosol localization uncertain and reports approved sperm-associated locations; tissue IHC has its own Enhanced reliability designation (HPA subcellular ICC-IF: location status; HPA tissue IHC: reliability). | Interpret each assay with its own cell identity, compartment and controls; use the separate IF/ICC guide for IF-specific decisions (standard IHC/IF practice). |
Experiment evidence
IHC & IF images showing HSPA5 staining
M00955 · IHCImmunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using the Antibody at 1:100 dilution.
M00955 · IHCImmunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.
M00955 · IFImmunofluorescent analysis using the Antibody at 1:50 dilution.
M00955-4 · IHCRat brain was stained with anti-GRP78/BiP rabbit antibody
M00955-4 · IHCHuman brain was stained with anti-GRP78/BiP rabbit antibody
M00955-1 · IHCImmunohistochemical analysis of paraffin-embedded human testis, using GRP78 BiP Antibody.
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000044574-HSPA5/tissue/cerebral+cortex#imid_10341713
HPA · Thyroid glandHuman Protein Atlas IHC, Thyroid gland. Source: https://www.proteinatlas.org/ENSG00000044574-HSPA5/tissue/thyroid+gland#imid_10341681
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000044574-HSPA5/tissue/colon#imid_10341655
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000044574-HSPA5/tissue/liver#imid_10341656
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA038845; green = HSPA5, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA038845; green = HSPA5, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for HSPA5 IHC & IF
🧪Run duodenum first: enterocytes should stain (HPA: High in duodenum enterocytes). HPA detects HSPA5 in all 45 scored tissues, so there is no supported negative tissue; use no-primary and isotype controls, and expect any unstained neighboring cells on the positive slide to show counterstain and background only, without treating them as proven HSPA5-negative cells (HPA: no negative tissues; standard IHC control practice).
Positive control tissue: Caudate (Neuronal cells, HPA High)
Negative control tissue: None in HPA: HSPA5 is detected in all 45 scored tissues. Use a no-primary (secondary-only) and an isotype control instead.
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show HSPA5 in A-431, U-251MG, U2OS, Sperm, with annotated localisation: Cytosol (uncertain), Annulus (approved) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only slide, an isotype control matched to the primary antibody’s host species and clonality, and HSPA5-knockout material or a peptide-block control when the immunizing peptide is available (standard IHC control practice). On duodenum, check endogenous peroxidase and, if using biotin-based detection, endogenous biotin; assess tissue autofluorescence if using IF (standard IHC/IF practice).
⚠️Feasibility: A paraffin-section IHC example is supplied at 1:100, but its fixative is unreported (M00955 tissue-IHC caption). No target-specific fixation window, fixation effect, or antigen-retrieval dependency is reported in the supplied evidence; assess retrieval on matched sections before scoring (supplied target/application evidence; standard IHC practice). The evidence does not establish that frozen sections or IF/ICC are easier; duodenal endogenous biotin can complicate biotin-based detection, and IF requires an autofluorescence control (standard IHC/IF practice).
HPA tissue IHC evidence for HSPA5
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — Medium consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Caudate | Neuronal cells | High | Protein (IHC) | HPA → |
| Cerebellum | Cells in molecular layer | High | Protein (IHC) | HPA → |
| Cerebral cortex | Neuronal cells | High | Protein (IHC) | HPA → |
| Colon | Endocrine cells | High | Protein (IHC) | HPA → |
| Duodenum | Enterocytes | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| None in HPA: HSPA5 is detected in all 45 scored tissues. Use a no-primary (secondary-only) and an isotype control instead. | ||||
Section 3
Advanced HSPA5 IHC Tips
Troubleshoot HSPA5 staining in paraffin sections by checking retrieval, compartment, controls and cell-specific scoring before interpreting signal intensity.
How should I retrieve HSPA5 in paraffin sections with weak staining?
Use citrate buffer at pH 6.0 for heat-induced retrieval at 95–98 °C for 20 minutes (page retrieval rule: cytoplasmic/membrane antigen). Allow sections to cool in buffer, then compare a known positive control alongside the test section under identical staining conditions (HPA: high in neuronal cells). If signal remains weak, trial another retrieval buffer or pH on serial sections, changing only that variable and checking for tissue damage (general IHC practice). HSPA5 is primarily an ER-lumen protein, so assess whether improved staining shows a plausible cytoplasmic distribution rather than diffuse nuclear color (UniProt P11021 localization; HPA: ubiquitous cytoplasmic expression).
Could fixation explain variable HSPA5 staining across my paraffin sections?
Target-specific fixation sensitivity is unknown from the supplied evidence; the catalog image identifies a paraffin-embedded section but does not state its fixative (M00955 caption: fixative not stated). Record the fixative, fixation duration and processing history for each specimen, then compare sections processed together with the same retrieval and detection conditions (general IHC practice). If fixation varies, test matched control material before attributing weaker color to reduced HSPA5 expression (general IHC practice). Avoid inferring fixation effects from the reported cytoplasmic staining pattern, ER localization or modified residues; those observations do not establish how this epitope responds to fixation (HPA: tissue profile; UniProt P11021 localization and modified residues).
Which HSPA5 staining patterns should I accept in tissue sections?
Expect predominantly cytoplasmic staining consistent with an ER-lumen chaperone; assess the signal within identifiable cells rather than treating every brown region as positive (UniProt P11021 localization; HPA: ubiquitous cytoplasmic expression). UniProt also reports cytoplasmic, melanosomal and context-dependent cell-surface localization, so an unusual pattern warrants confirmation with compartment markers and appropriate controls (UniProt P11021 localization). In the supplied tissue profile, neuronal cells and lung alveolar type II cells show high expression, whereas oral squamous epithelial cells show low expression (HPA: tissue IHC profile). Diffuse nuclear color or staining confined to tissue folds should prompt a check of morphology, retrieval and background before assigning biological meaning (general IHC practice).
How should epitope uncertainty affect my HSPA5 IHC interpretation?
The supplied record lists 0 isoforms and identifies a signal sequence at residues 1–18, followed by the mature chain at 19–654 (UniProt P11021 processing and isoforms). Without an antibody epitope or immunogen sequence, do not assume that the catalog antibody recognizes the mature chain, the signal sequence or a particular modified residue (supplied antibody evidence: epitope unspecified). HSPA5 has annotated modified residues, but their effect on this antibody’s tissue staining has not been established here (UniProt P11021 modified residues; supplied antibody evidence). If staining changes across conditions, compare serial sections and use an independently validated epitope or orthogonal assay before interpreting the difference as protein processing (general IHC practice).
How can I check HSPA5 localization by multiplex immunofluorescence?
For a separate IF experiment, pair HSPA5 with a validated marker of the cell type being examined, such as an appropriate neuronal marker when studying neuronal cells (HPA: high in neuronal cells; general IF practice). Choose fluorophores after measuring tissue autofluorescence, and include single-stain controls so bleed-through cannot mimic colocalization (general IF practice). Because the principal annotated pool lies in the ER lumen, test permeabilization conditions that give antibodies access across cellular and ER membranes while preserving morphology (UniProt P11021 localization; general IF practice). Compare intracellular signal with a no-primary control, and avoid treating apparent surface staining as established without a separately controlled surface-labeling experiment (UniProt P11021: context-dependent cell-surface localization; general IF practice).
What causes widespread brown background in HSPA5 chromogenic IHC?
First inspect a no-primary control and the section’s morphology: widespread color in both test and control suggests detection background rather than interpretable HSPA5 staining (general IHC practice). Check the peroxidase block, reagent concentration, wash stringency and DAB development time, adjusting one condition at a time (general chromogenic IHC practice). The catalog image uses a 1:100 antibody dilution in paraffin-embedded human non-Hodgkin’s lymphoma, which is a starting reference rather than proof of suitability for every specimen (M00955 caption). Because HSPA5 is broadly expressed, judge background against cell outlines and a matched low-expression compartment, not simply against an unstained tissue region (HPA: ubiquitous cytoplasmic expression; HPA: low in oral squamous epithelial cells).
How should I quantify HSPA5 across differently composed tissue sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and analysis area before scoring, then report the percentage of positive cells and an H-score based on intensity grades within that population (general IHC practice). Normalize comparisons to the number of evaluable cells or the measured tissue area; use positive-cell density per mm² when cell counts per area answer the biological question (general IHC practice). Apply one threshold, counterstain assessment and image-acquisition setting across comparison groups, excluding necrosis, folds and section edges by prespecified rules (general IHC practice). Report cell-type composition because HSPA5 expression varies by cell population, including high staining in neuronal cells and low staining in oral squamous epithelial cells (HPA: tissue IHC profile).
How can I distinguish true HSPA5 positivity from staining artefact?
Give greatest weight to cytoplasmic staining within intact, identifiable cells, consistent with HSPA5’s ER-lumen role and the tissue IHC profile (UniProt P11021 localization; HPA: ubiquitous cytoplasmic expression). Check whether the stained cell population fits the specimen: neuronal cells are reported high, while oral squamous epithelial cells are reported low (HPA: tissue IHC profile). Treat color concentrated at section edges, folds or necrotic areas as suspect, and compare it with a no-primary control and adjacent preserved tissue (general IHC practice). If color persists without primary antibody, investigate endogenous peroxidase or detection-reagent background before assigning a positive score (general chromogenic IHC practice).
Boster reagents
Best HSPA5 / Endoplasmic reticulum chaperone BiP IHC Antibodies
Anti-HSPA5 antibodies have IHC images from human lymphoma and testis and rat brain, plus an IF image from HepG2 cells (catalog image captions: M00955, M00955-1, M00955-4).
Real IHC data
Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
Cat # M00955
Real IHC data
Anti-GRP78/BiP Rabbit Monoclonal Antibody
Cat # M00955-4
Real IHC data
Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
Cat # M00955-1
M00955 has a paraffin-section IHC image of human non-Hodgkin’s lymphoma and an IF image of HepG2 cells (catalog image captions: M00955); M00955-4 has a rat-brain IHC image (catalog image caption: M00955-4). M00955-1 has a paraffin-section IHC image of human testis (catalog image caption: M00955-1).
Which to pick: For paraffin-section IHC, choose M00955 or M00955-1 based on their respective tissue images; neither caption reports the fixative (catalog IHC image captions: M00955, M00955-1). For IF/ICC, choose M00955, a rabbit monoclonal listed for both applications with a HepG2 IF image (catalog applications, clone and IF image caption: M00955). For IHC across species, M00955-4 lists human, mouse and rat reactivity and has IHC captions for all three; those captions do not report tissue processing or fixation (catalog reactivity and IHC image captions: M00955-4).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry P11021 (BIP_HUMAN, Endoplasmic reticulum chaperone BiP).
- Human Protein Atlas. HSPA5 tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. HSPA5 subcellular location (ICC-IF): Mainly localized to the cytosol and annulus. In addition localized to the mid piece, principal piece and end piece..
- Human Protein Atlas. HSPA5 antibody validation summary (3 antibodies).
- Elevated human placental heat shock protein 5 is associated with spontaneous preterm birth. Pediatric research 2023 — PMC9926443.
- Genetic and immunohistochemical analysis of HSPA5 in mouse and human retinas. Molecular vision 2016 — PMC5108462.
- Heat shock proteins related signature characterizes immune status and predicts prognosis of gliomas. Scientific reports 2025 — PMC12325653.
- Identification of robust diagnostic and prognostic gene signatures in different grades of gliomas: a retrospective study. PeerJ 2021 — PMC8121073.
- PubMed PMID:2840249 — UniProt-cited evidence.
- PubMed PMID:15164053 — UniProt-cited evidence.
- PubMed PMID:15489334 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for HSPA5 (Endoplasmic reticulum chaperone BiP). Expected localisation Cytoplasmic staining in tissue IHC (HPA tissue IHC). Antigen retrieval: Citrate pH 6.0 HIER, 95–98 °C, 20 min (rule: cytoplasmic / membrane antigen). Validated IHC antibody: M00955 at 1:50-1:200 (the product's own IHC validation image is shown). Controls (HPA): positive Caudate (Neuronal cells); negative None in HPA (detected in all 45 tissues); use no-primary + isotype controls. IHC workflow; specimen preparation unspecified. Protocol steps: 1. Sample: Paraffin-embedded tissue sections; fixative not specified (datasheet M00955; sample unspecified); 2. Fixation: Image fixative and duration unreported (datasheet M00955); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat-induced epitope retrieval in citrate buffer, pH 6.0 , 20 min at 95–98 °C…; 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% normal serum of the secondary host, 30 min , room temperature (standard); 8. Primary antibody: Rabbit monoclonal (clone BOO-8) anti-HSPA5, 1:50-1:200 (datasheet M00955); 9. Primary incubation: Overnight at 4 °C (standard). Watch for: Fixation: Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image M00955); Caveat: Ubiquitous expression limits negative tissue controls (HPA tissue IHC); Isoform / epitope: No isoforms annotated; mature chain spans aa 19–654 (UniProt); Regulation: Expression regulation is unreported (UniProt). Evidence: UniProt P11021, HPA tissue IHC · Enhanced, HPA ICC-IF · Mainly localized to the cytosol and annulus. In addition localized…, Datasheet M00955, PMC9926443, PMC8121073. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/HSPA5/immunohistochemistry-ihc-guide.