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Product Info Summary
| SKU: | M00955-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00955-1
BM4053 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody catalog # M00955-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00955-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AII-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GRP78 BiP
Reactive Species
M00955-1 is reactive to HSPA5 in Human, Mouse, Rat
Observed Molecular Weight
78 kDa
Calculated molecular weight
72.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00955-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: human testis tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human testis, using GRP78 BiP Antibody.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of GRP78 BiP expression in (1) HeLa cell lysate; (2) C6 cell lysate.
Specific Publications For Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody (M00955-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
Question
My boss were happy with the WB result of your anti-GRP78 BiP Rabbit Monoclonal antibody. However we have been able to see positive staining in liver endoplasmic reticulum lumen using this antibody. Is that expected? Could you tell me where is HSPA5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
From literature, liver does express HSPA5. Generally HSPA5 expresses in endoplasmic reticulum lumen. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-09
Question
We bought anti-GRP78 BiP Rabbit Monoclonal antibody for WB on articular cartilage a few years ago. I am using human, and We are going to use the antibody for IHC next. I would like examining articular cartilage as well as cajal-retzius cell fetal brain cortex in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
M. Wu
Verified customer
Asked: 2018-11-14
Answer
I took a look at the website and datasheets of our anti-GRP78 BiP Rabbit Monoclonal antibody and it appears that M00955-1 has been tested on human in both WB and IHC. Thus M00955-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-11-14
Question
We are currently using anti-GRP78 BiP Rabbit Monoclonal antibody M00955-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
The anti-GRP78 BiP Rabbit Monoclonal antibody (M00955-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-27
Question
We are interested in using your anti-GRP78 BiP Rabbit Monoclonal antibody for translocation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
G. Krishna
Verified customer
Asked: 2015-06-02
Answer
Thanks for your inquiry. This M00955-1 anti-GRP78 BiP Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-06-02
Question
We have seen staining in rat mammary carcinoma. Any tips? Is anti-GRP78 BiP Rabbit Monoclonal antibody supposed to stain mammary carcinoma positively?
S. Mitchell
Verified customer
Asked: 2015-03-24
Answer
According to literature mammary carcinoma does express HSPA5. According to Uniprot.org, HSPA5 is expressed in vena cava, cervix carcinoma, fibroblast, muscle, articular cartilage, mammary carcinoma, colon carcinoma, brain, cajal-retzius cell fetal brain cortex, melanoma, liver, among other tissues. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-03-24
