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Product Info Summary
| SKU: | M00955 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00955
BM4054 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody catalog # M00955. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00955)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BOO-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GRP78 BiP
Reactive Species
M00955 is reactive to HSPA5 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
72.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00955 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: Human non-Hodgkin's lymphoma tissue, Human thyroid cancer tissue, Human prostate cancer tissue, Rat testis tissue, Mouse skin tissue, human pancreas tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of GRP78 BiP expression in (1) LnCaP cell lysate;(2) HepG2 cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded human pancreas, using GRP78 BiP Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of HepG2 cells, using GRP78 BiP Antibody .
Specific Publications For Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody (M00955)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
Question
I am looking for using your anti-GRP78 BiP Rabbit Monoclonal antibody for toxin transport studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
We appreciate your inquiry. This M00955 anti-GRP78 BiP Rabbit Monoclonal antibody is tested on hepg2 cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-05
Question
We have observed staining in human brain. Any tips? Is anti-GRP78 BiP Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Based on literature brain does express HSPA5. Based on Uniprot.org, HSPA5 is expressed in vena cava, cervix carcinoma, fibroblast, muscle, articular cartilage, mammary carcinoma, colon carcinoma, brain, cajal-retzius cell fetal brain cortex, melanoma, liver, among other tissues. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-24
Question
We ordered your anti-GRP78 BiP Rabbit Monoclonal antibody for IF on muscle last year. I am using human, and I plan to use the antibody for IHC next. I am interested in examining muscle as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
I took a look at the website and datasheets of our anti-GRP78 BiP Rabbit Monoclonal antibody and I see that M00955 has been validated on human in both IF and IHC. Thus M00955 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-17
Question
My team were well pleased with the WB result of your anti-GRP78 BiP Rabbit Monoclonal antibody. However we have seen positive staining in liver endoplasmic reticulum lumen using this antibody. Is that expected? Could you tell me where is HSPA5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
From what I have seen in literature, liver does express HSPA5. Generally HSPA5 expresses in endoplasmic reticulum lumen. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-01
Question
We are currently using anti-GRP78 BiP Rabbit Monoclonal antibody M00955 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
The anti-GRP78 BiP Rabbit Monoclonal antibody (M00955) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-07
