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- Table of Contents
Facts about Nuclear distribution protein nudE-like 1.
Also positively regulates the activity of this minus-end directed microtubule motor protein dynein. May improve dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends.
Human | |
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Gene Name: | NDEL1 |
Uniprot: | Q9GZM8 |
Entrez: | 81565 |
Belongs to: |
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nudE family |
DKFZp451M0318; EOPA; MITAP1mitosin-associated protein MITAP1; Mitosin-associated protein 1; nuclear distribution protein nudE-like 1; nudE nuclear distribution gene E homolog (A. nidulans)-like 1; nudE nuclear distribution gene E homolog like 1 (A. nidulans); nudE nuclear distribution gene E homolog like 1; NUDELendooligopeptidase A; NUDE-like protein; Protein Nudel
Mass (kDA):
38.375 kDA
Human | |
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Location: | 17p13.1 |
Sequence: | 17; NC_000017.11 (8435884..8472744) |
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
In a previous article, we discussed the NDEL1 marker from Boster Bio's DISC1 ligand. Here, we'll discuss its unique features, including its sensitivity and interaction with DISC1. We will also talk about IHC-optimized Polyclonal Antibodies, which can help improve NDEL1 detectability. We will also discuss the interaction of the NDEL1 marker with DISC1, a protein found within the ER.
Picokine (tm) from Boster Bio is a high-sensitivity ELISA test kit. Picokine(tm), increases ELISA sensitivity up to the picogram level. This antibody was developed with picogram-level sensitive in mind. The kits can be validated for a variety if samples. On request, validation images are available. The Picoband is a polymer-based secondary antibody that saves you 30 minutes in IHC, and its technology is powered by insights into immunogen design. Technical support is available from BeNeLux distributor Sanbio.
Boster's Picokine ELISA kit uses proprietary coating and blocking technology. The systems are tested against biologically applicable matrices, such cell culture supernatants (CSC) and blood plasma. To ensure high inter and intra assay sensitivities, the kits go through extensive testing. For complete information on Boster's ELISA kits, visit their website. They can also provide information about custom service and BeNeLux shipping.
Researchers have recently reported that the NDEL1 gene interacts with DISC1 to suppress its activity. These interactions could have implications for SCZ vulnerability. These results highlight the importance of the protein in the formation of neural structures. Interestingly, NDEL1 is closely related to DISC1 which plays an important role in embryogenesis. As a result, the disruption of either of these proteins may result in changes in the formation of specific brain structures.
A fusion protein between NDEL1 and EGFP was generated and tested. Unlike NDEL1, EGFP could not inhibit MTOC translocation. Moreover, the fusion protein did not inhibit DISC1 or dynein translocation. However, dynein as well as DISC1 are associated with IS.
Mouse CTLs expressed two types of NDEL1 mRNA (NDEL1-1I and NDEL-1-Lis1). This protein is absent in human CTLs. However, it is present within all types of cells in mice. The NDE1 mouse isoforms had different molecular masses, and overlapped with DIC proteins and Lis1 proteins.
We first detected the presence of NDE1 in Jurkat cells. NDE1 EGFP and NDEL1 mEGFP were immunoprecipitated with Jurkat cells. Immunostaining Jurkat/Raji pairs of cells revealed that DIC as well NDE1 co-localize at IS. NDE1 did however not colocalize at the IS in Jurkat cells expressing DISC1mEGFP.
For the highest accuracy, IHC-optimized polyclonals are preferable to Western blotting methods. These antibodies bind to the protein in its natural form and thus achieve a higher affinity. IHC-validated antibodies are preferred, since the method of antigen retrieval can affect antibody recognition.
For best results, polyclonal antibodies should be diluted with their own buffer, preferably a 5% DMSO. DMSO should be used with a diluent containing 0.5% DMSO or an equivalent. Most antibodies require overnight incubation. Longer incubation periods can lead to non-specific signals.
Specificity is also important. Antibodies should recognize the target protein in the species of interest. In ideal cases, they will recognize the same epitope across different species of an organism. This will enable you identify the protein at the correct location and in the proper concentration. Once you have decided upon a specific epitope, the next step is to determine its target protein.
Cattoretti and colleagues describe an antigen unmasking technique. it is ideal to evaluate the mouse neovasculature. Anti-Factor VIII-related antibody and CD31 were not ideal for this because the antibodies used cross-reacted to mouse endothelium. Antibodies that specifically bind to the mouse endothelium will be critical in detecting angiogenesis of mouse xenografts.
PMID: 11163260 by Niethammer M., et al. NUDEL is a novel cdk5 substrate that associates with LIS1 and cytoplasmic dynein.
PMID: 12556484 by Yan X., et al. Human Nudel and NudE as regulators of cytoplasmic dynein in poleward protein transport along the mitotic spindle.