Anti-Nudel NDEL1 Antibody

Product Name

Anti-Nudel NDEL1 Antibody

View all NDEL1 Antibodies

SKU/Catalog Number

A02478

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Nudel NDEL1 Antibody (Catalog # A02478). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Nudel antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Nudel NDEL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02478)

Host

Rabbit

Contents

Nudel Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Nudel antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Nudel. The immunogen is located within the first 50 amino acids of Nudel.

Reactive Species

A02478 is reactive to NDEL1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

38.4 kDa

Background of NDEL1

Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration. It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration; phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology. It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions. Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02478 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Nudel antibody can be used for detection of Nudel by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NDEL1

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