Nudel (NDEL1) (NM_001025579) Human Over-expression Lysate

Lysate of Nudel over-expression cell lines, WB control

Transient overexpression lysate of nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1

Product Info Summary

SKU: LS081565
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1

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Product Name

Nudel (NDEL1) (NM_001025579) Human Over-expression Lysate

See all Nudel products

SKU/Catalog Number

LS081565

Size

100 µg

Description

Transient overexpression lysate of nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1

Storage & Handling

Cite This Product

Nudel (NDEL1) (NM_001025579) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081565)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for NDEL1 (Source: Uniprot.org, NCBI)

Gene Name

NDEL1

Full Name

Nuclear distribution protein nudE-like 1

Weight

38.375kDa

Superfamily

nudE family

Alternative Names

DKFZp451M0318; EOPA; MITAP1mitosin-associated protein MITAP1; Mitosin-associated protein 1; nuclear distribution protein nudE-like 1; nudE nuclear distribution gene E homolog (A. nidulans)-like 1; nudE nuclear distribution gene E homolog like 1 (A. nidulans); nudE nuclear distribution gene E homolog like 1; NUDELendooligopeptidase A; NUDE-like protein; Protein Nudel NDEL1 EOPA, MITAP1, NDE1L1, NDE2, NUDEL nudE neurodevelopment protein 1 like 1 nuclear distribution protein nudE-like 1|endooligopeptidase A|mitosin-associated protein 1|mitosin-associated protein MITAP1|nudE nuclear distribution E homolog-like 1|nudE nuclear distribution gene E homolog-like 1|protein Nudel

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on NDEL1, check out the NDEL1 Infographic

NDEL1 infographic

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In this infographic, you will see the following information for NDEL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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