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- Table of Contents
NOS1 · Western blot design guide
NOS1 Western Blot Planning Guide
Plan a NOS1 Western blot around the catalog-observed 161 kDa band, image-backed A01070 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
NOS1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 161 kDa observed | |
| Observed band | 161 kDa observed | |
| Gel | 8-10% | |
| Positive control | Caudate | |
| Negative control | Adipose tissue |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 161 kDa |
| ⓘ | Localization | Cell membrane, sarcolemma / Cell projection, dendritic spine |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated NOS1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Caudate |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01070 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected NOS1 Western Blot Band Size?
Use the product-observed 161 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 161 kDa | Matches the authoritative product WB observation. |
| 161 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected NOS1 appearancePlan around 161 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 161 kDa; use this as the primary experimental expectation. |
| Calculated mass | 161 kDa from UniProt P29475; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01070. |
| Situation | Likely cause | Next action |
|---|---|---|
| 161 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing NOS1 band
A01070 · NOS1Observed 161 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for NOS1 Western blot
🧪Use Caudate as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Caudate (High)
Negative control: Adipose tissue (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for NOS1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Caudate | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Hippocampus | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced NOS1 Western Blot Tips
Deeper troubleshooting and optimisation questions for NOS1, answered from its protein features.
Which band should guide the blot?
Use 161 kDa, the observation attached to the authoritative A01070 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 161 kDa expectation.
Which positive control should I start with?
Start with Caudate, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adipose tissue as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for NOS1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01070 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
NOS1 Western Blot Reagents
Human/Mouse/Rat-reactive NOS1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-nNOS (neuronal)/NOS1 Antibody Picoband®
Cat # A01070
Real WB data
Anti-nNOS (neuronal)/NOS1 Antibody Picoband®
Cat # A01070-2
Real WB data
Anti-nNOS (neuronal)/NOS1 Antibody Picoband®
Cat # PA1329
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.