Anti-nNOS (neuronal)/NOS1 Antibody Picoband™

nNOS antibody

Boster Bio Anti-nNOS (neuronal)/NOS1 Antibody Picoband™ catalog # A01070. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 8 publication(s).

Product Info Summary

SKU: A01070
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-nNOS (neuronal)/NOS1 Antibody Picoband™

View all nNOS Antibodies

SKU/Catalog Number

A01070

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-nNOS (neuronal)/NOS1 Antibody Picoband™ catalog # A01070. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-nNOS (neuronal)/NOS1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01070)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS(neuronal), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01070 is reactive to NOS1 in Human, Mouse, Rat

Applications

A01070 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

160 kDa

Calculated molecular weight

160.97kDa

Background of nNOS

Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Validation Images & Assay Conditions

Gene/Protein Information For NOS1 (Source: Uniprot.org, NCBI)

Gene Name

NOS1

Full Name

Nitric oxide synthase, brain

Weight

160.97kDa

Superfamily

NOS family

Alternative Names

bNOS; brain; Constitutive NOS; EC 1.14.13.39; IHPS1; Neuronal NOS; nitric oxide synthase 1 (neuronal); nNOS; N-NOS; nNOSNOS type I; NOS; NOS1; Peptidyl-cysteine S-nitrosylase NOS1 NOS1 IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS nitric oxide synthase 1 nitric oxide synthase, brain|NOS type I|constitutive NOS|neuronal NOS|nitric oxide synthase 1 (neuronal)|peptidyl-cysteine S-nitrosylase NOS1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NOS1, check out the NOS1 Infographic

NOS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NOS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01070 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Singh S,Kumar V,Kumar N,Sharma P,Waheed SM.Protective and Modulatory Effects of Trapa bispinosa and Trigonella foenum-graecum on Neuroblastoma Cells Through Neuronal Nitric Oxide Synthase.Assay Drug Dev Technol.2020 Jan;18(1):64-74.doi:10.1089/adt.2018.91
Species: Mouse
A01070 usage in article: APP:WB, SAMPLE:NB-41 CELL, DILUTION:1:1000

George AK, Behera J, Kelly KE, Mondal NK, Richardson KP, Tyagi N. Sci Rep. 2018 Mar 26;8(1):5158. doi: 10.1038/s41598-018-23568-z. Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling.

Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation

Distribution of constitutive nitric oxide synthase in the jejunum of adult rat

Kong L, Wei Q, Fedail Js, Shi F, Nagaoka K, Watanabe G. J Reprod Dev. 2015;61(3):219-27. Doi: 10.1262/Jrd.2014-129. Epub 2015 Mar 20. Effects Of Thyroid Hormones On The Antioxidative Status In The Uterus Of Young Adult Rats.

Zhang W, Wei Qw, Wang Zc, Ding W, Wang W, Shi Fx. J Zhejiang Univ Sci B. 2011 Jan;12(1):55-64. Doi: 10.1631/Jzus.B1000174. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And The Related Nitric Oxide/Cyclic Gmp Si...

Wu R, Yin J, Yao K, Haefliger I. Mol Vis. 2007 Apr 2;13:493-6. Inhibition By Brimonidine Of Forskolin-Induced Nitric Oxide Synthase Expression In Human Ciliary Bodies In Vitro.

Chang Mj, Xiao Jh, Wang Y, Yan Yl, Yang J, Wang Jl. Plos One. 2012;7(12):E50291. Doi: 10.1371/Journal.Pone.0050291. Epub 2012 Dec 3. 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-Beta-D-Glucoside Improves Gastrointestinal Motility Disorders In Stz-Induced ...

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4 Customer Q&As for Anti-nNOS (neuronal)/NOS1 Antibody Picoband™

Question

My question regards using your anti-nNOS (neuronal)/NOS1 antibody for negative regulation of calcium ion transport studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

We appreciate your inquiry. This A01070 anti-nNOS (neuronal)/NOS1 antibody is tested on mouse brain. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-31

Question

Our lab were content with the WB result of your anti-nNOS (neuronal)/NOS1 antibody. However we have observed positive staining in skeletal muscle cell membrane using this antibody. Is that expected? Could you tell me where is NOS1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-04-27

Answer

From what I have seen in literature, skeletal muscle does express NOS1. Generally NOS1 expresses in cell membrane, sarcolemma. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048

Boster Scientific Support

Answered: 2018-04-27

Question

We are currently using anti-nNOS (neuronal)/NOS1 antibody A01070 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

The anti-nNOS (neuronal)/NOS1 antibody (A01070) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-19

Question

We have seen staining in mouse vastus lateralis. Are there any suggestions? Is anti-nNOS (neuronal)/NOS1 antibody supposed to stain vastus lateralis positively?

D. Krishna

Verified customer

Asked: 2016-06-03

Answer

From literature vastus lateralis does express NOS1. From Uniprot.org, NOS1 is expressed in vastus lateralis, cerebellum, brain, retina, testis, skeletal muscle, among other tissues. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048

Boster Scientific Support

Answered: 2016-06-03

Order DetailsPrice
A01070

100μg

$370
A01070-10ug

10μg sample (liquid)

$99
A01070-Biotin

100 μg Biotin conjugated

$570
A01070-Cy3

100 μg Cy3 conjugated

$570
A01070-Dylight488

100 μg Dylight488 conjugated

$570
A01070-Dylight550

100 μg Dylight550 conjugated

$570
A01070-Dylight594

100 μg Dylight594 conjugated

$570
A01070-FITC

100 μg FITC conjugated

$570
A01070-HRP

100 μg HRP conjugated

$570
A01070-APC

100 μg APC conjugated

$670
A01070-PE

100 μg PE conjugated

$670
A01070-carrier-free

Carrier Free

$370

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