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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-nNOS (neuronal)/NOS1 Antibody
Boster Bio Anti-nNOS (neuronal)/NOS1 Antibody catalog # PA1329. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-nNOS (neuronal)/NOS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1329)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS (neuronal) (1418-1434aa IAFIEESKKDTDEVFSS), identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1329 is reactive to NOS1 in Human, Mouse, Rat
PA1329 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-nNOS(neuronal) antibody, PA1329, Western blotting
All lanes: Anti nNOS(neuronal)(PA1329) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 160KD
Observed bind size: 160KD
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Anti-nNOS(neuronal) antibody, PA1329, IHC(P)
IHC(P): Rat Brain Tissue
Gene/Protein Information For NOS1 (Source: Uniprot.Org, NCBI)
Nitric oxide synthase, brain
bNOS; brain; Constitutive NOS; EC 184.108.40.206; IHPS1; Neuronal NOS; nitric oxide synthase 1 (neuronal); nNOS; N-NOS; nNOSNOS type I; NOS; NOS1; Peptidyl-cysteine S-nitrosylase NOS1 NOS1 IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS nitric oxide synthase 1 nitric oxide synthase, brain|NOS type I|constitutive NOS|neuronal NOS|nitric oxide synthase 1 (neuronal)|peptidyl-cysteine S-nitrosylase NOS1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on NOS1, check out the NOS1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NOS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1329 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Singh S,Kumar V,Kumar N,Sharma P,Waheed SM.Protective and Modulatory Effects of Trapa bispinosa and Trigonella foenum-graecum on Neuroblastoma Cells Through Neuronal Nitric Oxide Synthase.Assay Drug Dev Technol.2020 Jan;18(1):64-74.doi:10.1089/adt.2018.91
PA1329 usage in article: APP:WB, SAMPLE:NB-41 CELL, DILUTION:1:1000
Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation
Distribution of constitutive nitric oxide synthase in the jejunum of adult rat
Ding J, Tang Y, Tang Z, Zhang X, Wang G. Med Sci Monit. 2017 Feb 20;23:929-937. A Variant in the Precursor of MicroRNA-146a is Responsible for Development of Erectile Dysfunction in Patients with Chronic Prostatitis via Targeting NOS1
Kong L, Wei Q, Fedail Js, Shi F, Nagaoka K, Watanabe G. J Reprod Dev. 2015;61(3):219-27. Doi: 10.1262/Jrd.2014-129. Epub 2015 Mar 20. Effects Of Thyroid Hormones On The Antioxidative Status In The Uterus Of Young Adult Rats.
Liu R, Li Yp, Zhang Wg, Fu Qq, Liu N, Zhou Gh. Meat Sci. 2015 Jan;99:25-31. Doi: 10.1016/J.Meatsci.2014.08.010. Epub 2014 Aug 27. Activity And Expression Of Nitric Oxide Synthase In Pork Skeletal Muscles.
Xu M, Wei Q, Zheng K, Mao D, Zheng Y, Li Y, Shi F. Acta Histochem. 2014 Oct;116(8):1323-30. Doi: 10.1016/J.Acthis.2014.08.003. Epub 2014 Sep 1. Protective Effects Of Big-Leaf Mulberry And Physiological Roles Of Nitric Oxide Synthases In The Testis...
Zhang W, Wei Qw, Wang Zc, Ding W, Wang W, Shi Fx. J Zhejiang Univ Sci B. 2011 Jan;12(1):55-64. Doi: 10.1631/Jzus.B1000174. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And The Related Nitric Oxide/Cyclic Gmp Si...
Li Y, Zhou X, Wei Qw, Huang Rh, Shi Fx. Acta Histochem. 2014 Apr;116(3):466-73. Doi: 10.1016/J.Acthis.2013.10.003. Epub 2013 Nov 13. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And Soluble Guanylyl Cyclase ?? ...
Wu R, Yin J, Yao K, Haefliger I. Mol Vis. 2007 Apr 2;13:493-6. Inhibition By Brimonidine Of Forskolin-Induced Nitric Oxide Synthase Expression In Human Ciliary Bodies In Vitro.
Ding W, Zhang W, Hui Fm, Zhang Yh, Zhang Ff, Li Xm, Shi Fx. Anim Reprod Sci. 2012 Apr;131(3-4):172-80. Doi: 10.1016/J.Anireprosci.2012.02.013. Epub 2012 Mar 7. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And S...
Zheng K, Sulieman Fj, Li J, Wei Q, Xu M, Shi F. Reprod Biol. 2015 Mar;15(1):27-33. Doi: 10.1016/J.Repbio.2014.11.002. Epub 2014 Nov 20. Nitric Oxide And Thyroid Hormone Receptor Alpha 1 Contribute To Ovarian Follicular Development In Immature Hype...
Fedail Js, Zheng K, Wei Q, Kong L, Shi F. Endocrine. 2014 Aug;46(3):594-604. Doi: 10.1007/S12020-013-0092-Y. Epub 2013 Nov 20. Roles Of Thyroid Hormones In Follicular Development In The Ovary Of Neonatal And Immature Rats.
Yuan Zr, Liu B, Zhang Y, Yuan L, Muteliefu G, Lu J. Brain Res. 2004 May 15;1008(1):1-10. Upregulated Expression Of Neuronal Nitric Oxide Synthase By Insulin In Both Neurons And Astrocytes.
Wang J, Yan L, Zhao X, Wu W, Zhou Lh. Nitric Oxide. 2010 Jan 1;22(1):37-42. Doi: 10.1016/J.Niox.2009.11.005. Epub 2009 Dec 3. The Diversity Of Nnos Gene Expression In Avulsion-Injured Spinal Motoneurons Among Laboratory Rodents.
Chang Mj, Xiao Jh, Wang Y, Yan Yl, Yang J, Wang Jl. Plos One. 2012;7(12):E50291. Doi: 10.1371/Journal.Pone.0050291. Epub 2012 Dec 3. 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-Beta-D-Glucoside Improves Gastrointestinal Motility Disorders In Stz-Induced ...
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