Anti-nNOS (neuronal)/NOS1 Antibody

Boster Bio Anti-nNOS (neuronal)/NOS1 Antibody catalog # PA1329. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 16 publication(s).

Product Info Summary

SKU: PA1329
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-nNOS (neuronal)/NOS1 Antibody

See all nNOS products

SKU/Catalog Number

PA1329

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-nNOS (neuronal)/NOS1 Antibody catalog # PA1329. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-nNOS (neuronal)/NOS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1329)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS (neuronal) (1418-1434aa IAFIEESKKDTDEVFSS), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1329 is reactive to NOS1 in Human, Mouse, Rat

Applications

PA1329 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For NOS1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P29475

Gene ID

4842

Gene Name

NOS1

Full Name

Nitric oxide synthase, brain

Weight

160.97kDa

Superfamily

NOS family

Alternative Names

bNOS; brain; Constitutive NOS; EC 1.14.13.39; IHPS1; Neuronal NOS; nitric oxide synthase 1 (neuronal); nNOS; N-NOS; nNOSNOS type I; NOS; NOS1; Peptidyl-cysteine S-nitrosylase NOS1 NOS1 IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS nitric oxide synthase 1 nitric oxide synthase, brain|NOS type I|constitutive NOS|neuronal NOS|nitric oxide synthase 1 (neuronal)|peptidyl-cysteine S-nitrosylase NOS1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NOS1, check out the NOS1 Infographic

NOS1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NOS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1329 has been cited in 16 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Singh S,Kumar V,Kumar N,Sharma P,Waheed SM.Protective and Modulatory Effects of Trapa bispinosa and Trigonella foenum-graecum on Neuroblastoma Cells Through Neuronal Nitric Oxide Synthase.Assay Drug Dev Technol.2020 Jan;18(1):64-74.doi:10.1089/adt.2018.91
Species: Mouse
PA1329 usage in article: APP:WB, SAMPLE:NB-41 CELL, DILUTION:1:1000

Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation

Distribution of constitutive nitric oxide synthase in the jejunum of adult rat

Ding J, Tang Y, Tang Z, Zhang X, Wang G. Med Sci Monit. 2017 Feb 20;23:929-937. A Variant in the Precursor of MicroRNA-146a is Responsible for Development of Erectile Dysfunction in Patients with Chronic Prostatitis via Targeting NOS1

Kong L, Wei Q, Fedail Js, Shi F, Nagaoka K, Watanabe G. J Reprod Dev. 2015;61(3):219-27. Doi: 10.1262/Jrd.2014-129. Epub 2015 Mar 20. Effects Of Thyroid Hormones On The Antioxidative Status In The Uterus Of Young Adult Rats.

Liu R, Li Yp, Zhang Wg, Fu Qq, Liu N, Zhou Gh. Meat Sci. 2015 Jan;99:25-31. Doi: 10.1016/J.Meatsci.2014.08.010. Epub 2014 Aug 27. Activity And Expression Of Nitric Oxide Synthase In Pork Skeletal Muscles.

Xu M, Wei Q, Zheng K, Mao D, Zheng Y, Li Y, Shi F. Acta Histochem. 2014 Oct;116(8):1323-30. Doi: 10.1016/J.Acthis.2014.08.003. Epub 2014 Sep 1. Protective Effects Of Big-Leaf Mulberry And Physiological Roles Of Nitric Oxide Synthases In The Testis...

Zhang W, Wei Qw, Wang Zc, Ding W, Wang W, Shi Fx. J Zhejiang Univ Sci B. 2011 Jan;12(1):55-64. Doi: 10.1631/Jzus.B1000174. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And The Related Nitric Oxide/Cyclic Gmp Si...

Li Y, Zhou X, Wei Qw, Huang Rh, Shi Fx. Acta Histochem. 2014 Apr;116(3):466-73. Doi: 10.1016/J.Acthis.2013.10.003. Epub 2013 Nov 13. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And Soluble Guanylyl Cyclase ?? ...

Wu R, Yin J, Yao K, Haefliger I. Mol Vis. 2007 Apr 2;13:493-6. Inhibition By Brimonidine Of Forskolin-Induced Nitric Oxide Synthase Expression In Human Ciliary Bodies In Vitro.

Ding W, Zhang W, Hui Fm, Zhang Yh, Zhang Ff, Li Xm, Shi Fx. Anim Reprod Sci. 2012 Apr;131(3-4):172-80. Doi: 10.1016/J.Anireprosci.2012.02.013. Epub 2012 Mar 7. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And S...

Zheng K, Sulieman Fj, Li J, Wei Q, Xu M, Shi F. Reprod Biol. 2015 Mar;15(1):27-33. Doi: 10.1016/J.Repbio.2014.11.002. Epub 2014 Nov 20. Nitric Oxide And Thyroid Hormone Receptor Alpha 1 Contribute To Ovarian Follicular Development In Immature Hype...

Fedail Js, Zheng K, Wei Q, Kong L, Shi F. Endocrine. 2014 Aug;46(3):594-604. Doi: 10.1007/S12020-013-0092-Y. Epub 2013 Nov 20. Roles Of Thyroid Hormones In Follicular Development In The Ovary Of Neonatal And Immature Rats.

Yuan Zr, Liu B, Zhang Y, Yuan L, Muteliefu G, Lu J. Brain Res. 2004 May 15;1008(1):1-10. Upregulated Expression Of Neuronal Nitric Oxide Synthase By Insulin In Both Neurons And Astrocytes.

Wang J, Yan L, Zhao X, Wu W, Zhou Lh. Nitric Oxide. 2010 Jan 1;22(1):37-42. Doi: 10.1016/J.Niox.2009.11.005. Epub 2009 Dec 3. The Diversity Of Nnos Gene Expression In Avulsion-Injured Spinal Motoneurons Among Laboratory Rodents.

Chang Mj, Xiao Jh, Wang Y, Yan Yl, Yang J, Wang Jl. Plos One. 2012;7(12):E50291. Doi: 10.1371/Journal.Pone.0050291. Epub 2012 Dec 3. 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-Beta-D-Glucoside Improves Gastrointestinal Motility Disorders In Stz-Induced ...

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5 Customer Q&As for Anti-nNOS (neuronal)/NOS1 Antibody

Question

We ordered your anti-nNOS (neuronal)/NOS1 antibody for IHC on cerebellum in the past. I am using human, and We are going to use the antibody for WB next. I am interested in examining cerebellum as well as retina in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

I have checked the website and datasheets of our anti-nNOS (neuronal)/NOS1 antibody and it appears that PA1329 has been validated on human in both IHC and WB. Thus PA1329 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-11

Question

My boss were happy with the WB result of your anti-nNOS (neuronal)/NOS1 antibody. However we have observed positive staining in vastus lateralis sarcolemma using this antibody. Is that expected? Could you tell me where is NOS1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

Based on literature, vastus lateralis does express NOS1. Generally NOS1 expresses in cell membrane, sarcolemma. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048

Boster Scientific Support

Answered: 2019-12-03

Question

I am interested in using your anti-nNOS (neuronal)/NOS1 antibody for positive regulation of sodium ion transmembrane transport studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

Thank you for your inquiry. This PA1329 anti-nNOS (neuronal)/NOS1 antibody is validated on rat brain tissue, tissue lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-07

Question

We have observed staining in rat cerebellum. What should we do? Is anti-nNOS (neuronal)/NOS1 antibody supposed to stain cerebellum positively?

B. Wu

Verified customer

Asked: 2019-05-03

Answer

From what I have seen in literature cerebellum does express NOS1. From what I have seen in Uniprot.org, NOS1 is expressed in vastus lateralis, cerebellum, brain, retina, testis, skeletal muscle, among other tissues. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048

Boster Scientific Support

Answered: 2019-05-03

Question

We are currently using anti-nNOS (neuronal)/NOS1 antibody PA1329 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

C. Johnson

Verified customer

Asked: 2016-06-16

Answer

The anti-nNOS (neuronal)/NOS1 antibody (PA1329) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-06-16

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