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- Table of Contents
NOS1 antibody (PA1329) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 16 Publications
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Anti-nNOS(neuronal) antibody, PA1329, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
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Anti-nNOS (neuronal)/NOS1 Antibody Picoband®
NOS1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
NOS1 antibody was positively detected by WB in:
Rat Brain Tissue, MCF-7 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NOS1 antibody was positively detected by IHC in:
Rat Brain Tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Rat, Human, Mouse
Western blot
0.1-0.5μg/ml
Human, Rat, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Human
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-nNOS (neuronal)/NOS1 Antibody Picoband®
View all NOS1 Antibodies
View all NOS1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS(neuronal), identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
160 kDa
Calculated Molecular Weight
161.0 kDa
Background of NOS1
Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.
Cite This Product
Anti-nNOS (neuronal)/NOS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1329)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Rat
94%
High Homology
Zebrafish
82%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NOS1 antibody
Immunohistochemistry (IHC) protocol for NOS1 antibody
Publications
Application:
Species:
Singh S,Kumar V,Kumar N,Sharma P,Waheed SM.Protective and Modulatory Effects of Trapa bispinosa and Trigonella foenum-graecum on Neuroblastoma Cells Through Neuronal Nitric Oxide Synthase.Assay Drug Dev Technol.2020 Jan;18(1):64-74.doi:10.1089/adt.2018.91
Species: Mouse
PMID: 31742429
Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation
PMID: 29207043
Distribution of constitutive nitric oxide synthase in the jejunum of adult rat
PMID: 12046087
Ding J, Tang Y, Tang Z, Zhang X, Wang G. Med Sci Monit. 2017 Feb 20;23:929-937. A Variant in the Precursor of MicroRNA-146a is Responsible for Development of Erectile Dysfunction in Patients with Chronic Prostatitis via Targeting NOS1
PMID: 28218896
Kong L, Wei Q, Fedail Js, Shi F, Nagaoka K, Watanabe G. J Reprod Dev. 2015;61(3):219-27. Doi: 10.1262/Jrd.2014-129. Epub 2015 Mar 20. Effects Of Thyroid Hormones On The Antioxidative Status In The Uterus Of Young Adult Rats.
PMID: 25797533
Liu R, Li Yp, Zhang Wg, Fu Qq, Liu N, Zhou Gh. Meat Sci. 2015 Jan;99:25-31. Doi: 10.1016/J.Meatsci.2014.08.010. Epub 2014 Aug 27. Activity And Expression Of Nitric Oxide Synthase In Pork Skeletal Muscles.
PMID: 25280359
Xu M, Wei Q, Zheng K, Mao D, Zheng Y, Li Y, Shi F. Acta Histochem. 2014 Oct;116(8):1323-30. Doi: 10.1016/J.Acthis.2014.08.003. Epub 2014 Sep 1. Protective Effects Of Big-Leaf Mulberry And Physiological Roles Of Nitric Oxide Synthases In The Testis...
PMID: 25183664
Zhang W, Wei Qw, Wang Zc, Ding W, Wang W, Shi Fx. J Zhejiang Univ Sci B. 2011 Jan;12(1):55-64. Doi: 10.1631/Jzus.B1000174. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And The Related Nitric Oxide/Cyclic Gmp Si...
PMID: 21194187
Li Y, Zhou X, Wei Qw, Huang Rh, Shi Fx. Acta Histochem. 2014 Apr;116(3):466-73. Doi: 10.1016/J.Acthis.2013.10.003. Epub 2013 Nov 13. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And Soluble Guanylyl Cyclase ?? ...
PMID: 24238988
Wu R, Yin J, Yao K, Haefliger I. Mol Vis. 2007 Apr 2;13:493-6. Inhibition By Brimonidine Of Forskolin-Induced Nitric Oxide Synthase Expression In Human Ciliary Bodies In Vitro.
PMID: 17417610
Ding W, Zhang W, Hui Fm, Zhang Yh, Zhang Ff, Li Xm, Shi Fx. Anim Reprod Sci. 2012 Apr;131(3-4):172-80. Doi: 10.1016/J.Anireprosci.2012.02.013. Epub 2012 Mar 7. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And S...
PMID: 22498451
Zheng K, Sulieman Fj, Li J, Wei Q, Xu M, Shi F. Reprod Biol. 2015 Mar;15(1):27-33. Doi: 10.1016/J.Repbio.2014.11.002. Epub 2014 Nov 20. Nitric Oxide And Thyroid Hormone Receptor Alpha 1 Contribute To Ovarian Follicular Development In Immature Hype...
PMID: 25726374
Fedail Js, Zheng K, Wei Q, Kong L, Shi F. Endocrine. 2014 Aug;46(3):594-604. Doi: 10.1007/S12020-013-0092-Y. Epub 2013 Nov 20. Roles Of Thyroid Hormones In Follicular Development In The Ovary Of Neonatal And Immature Rats.
PMID: 24254997
Yuan Zr, Liu B, Zhang Y, Yuan L, Muteliefu G, Lu J. Brain Res. 2004 May 15;1008(1):1-10. Upregulated Expression Of Neuronal Nitric Oxide Synthase By Insulin In Both Neurons And Astrocytes.
PMID: 15081376
Wang J, Yan L, Zhao X, Wu W, Zhou Lh. Nitric Oxide. 2010 Jan 1;22(1):37-42. Doi: 10.1016/J.Niox.2009.11.005. Epub 2009 Dec 3. The Diversity Of Nnos Gene Expression In Avulsion-Injured Spinal Motoneurons Among Laboratory Rodents.
PMID: 19931630
Chang Mj, Xiao Jh, Wang Y, Yan Yl, Yang J, Wang Jl. Plos One. 2012;7(12):E50291. Doi: 10.1371/Journal.Pone.0050291. Epub 2012 Dec 3. 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-Beta-D-Glucoside Improves Gastrointestinal Motility Disorders In Stz-Induced ...
PMID: 23226517
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Product Promise
We promise Anti-nNOS (neuronal)/NOS1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1329
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026