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PDCD5 / Programmed cell death protein 5 · IHC design guide
Design Immunohistochemistry for PDCD5
Plan PDCD5 IHC in paraffin sections using the catalog antibody A02613-1 (datasheet A02613-1). Use HPA’s cytoplasmic tissue staining pattern to select controls, and interpret unexpected staining with its reported off-target binding in mind (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
PDCD5 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasm in tissue sections (HPA tissue IHC) | |
| Staining pattern | Ubiquitous cytoplasmic staining across cell types (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet A02613-1) | |
| Positive control | Placenta+4 more · see all | |
| Negative control | Caudate+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent across sections (standard IHC practice; not target-specific) | |
| Caveat | Presumed off-target staining may mimic PDCD5 (HPA tissue IHC) | |
| Regulation | Genotoxic stress: nuclear accumulation (UniProt) | |
| Isoform / epitope | 2 isoforms; epitope impact unspecified (UniProt) |
Section 1
Recommended PDCD5 IHC & IF Protocols
Compare the catalog antibody’s IHC-P protocol with four published PDCD5 paraffin-section IHC protocols (datasheet A02613-1; PMC4937001; PMC3939472; PMC13344223; PMC4832950).
| Sample | Paraffin-embedded human stomach tissue; fixative not specified (datasheet A02613-1) |
| Fixation | Image fixative and duration unreported (datasheet A02613-1); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet A02613-1); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet A02613-1) |
| Primary antibody | Rabbit anti-PDCD5, 1:50 recommended; image 2 μg/ml (datasheet A02613-1) |
| Primary incubation | Overnight at 4 °C (datasheet A02613-1) |
| Detection | HRP-conjugated secondary, DAB chromogen (datasheet A02613-1) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | PDCD5-positive staining in cytotrophoblasts of placenta (HPA tissue IHC: High). HPA tissue profile: Ubiquitous cytoplasmic expression. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA pH 8.0 retrieval for the catalog antibody (datasheet A02613-1); sodium citrate pH 6.0 is a published alternative (PMC3939472).
Section 2
What Is the Expected PDCD5 Staining Pattern?
PDCD5 is mainly cytosolic, with nuclear localisation also reported; UniProt notes nuclear accumulation after genotoxic stress (HPA subcellular; UniProt O14737). In tissue IHC, expect broad cytoplasmic staining, including high staining in placental cytotrophoblasts, skin endothelial cells and late spermatids (HPA tissue IHC). Interpret exceptions cautiously: HPA rates tissue evidence Enhanced but reports medium RNA–staining consistency and disregarded presumed off-target binding (HPA tissue IHC). PDCD5 has no transmembrane segment (UniProt O14737 topology).
| Cytoplasmic staining in the expected cells, especially strong staining in placental cytotrophoblasts, skin endothelial cells or late spermatids. | This fits the reported ubiquitous cytoplasmic tissue pattern and the high levels assigned to those cell populations (HPA tissue IHC). Score the identified cell population and its compartment, rather than treating every stained structure in the section as equivalent (general IHC practice). |
| Predominantly nuclear staining, or a sharp membrane rim, with little cytoplasmic signal. | A membrane-only pattern conflicts with the reported cytosolic localisation and lack of a transmembrane segment (HPA subcellular; UniProt O14737 topology). Nuclear signal can be plausible because nucleoplasmic localisation is approved and nuclear accumulation follows genotoxic stress; assess context and controls before calling it artefact (HPA subcellular; UniProt O14737). |
| Strong staining in a cell population reported as not detected, such as heart cardiomyocytes or prostate glandular cells. | Treat an isolated unexpected result as a specificity or detection warning, not proof of expression: HPA reports those populations as not detected and notes presumed off-target binding in its tissue assessment (HPA tissue IHC). Check morphology, controls and whether the stain follows a plausible cellular compartment (general IHC practice). |
| Uniform colour across cells and empty spaces, or granular deposits that obscure cellular boundaries. | This is difficult to score as PDCD5 because the reported tissue pattern is cellular and cytoplasmic (HPA tissue IHC). Broad haze or deposits can arise from background or chromogen handling; compare a no-primary control and inspect the section for discrete intracellular signal (general IHC practice). |
| No convincing staining in placental cytotrophoblasts, skin endothelial cells or late spermatids. | These are high-staining populations in the HPA tissue record, so absence warrants a technical check before interpreting the specimen as negative (HPA tissue IHC). Verify the expected cells are present, then review section quality, retrieval, antibody incubation and detection with a suitable positive control (general IHC practice). |
💡Expected PDCD5 appearanceCall a result consistent with PDCD5 when identifiable placental cytotrophoblasts, skin endothelial cells or late spermatids show high cytoplasmic staining (HPA tissue IHC); diffuse extracellular colour, a membrane-only rim or strong signal confined to reported negative cells is a warning pattern (HPA tissue IHC; HPA subcellular; UniProt O14737 topology; general IHC practice).
| Cell population and tissue context | HPA reports high staining in placental cytotrophoblasts, skin endothelial cells and late spermatids, but no detection in heart cardiomyocytes (HPA tissue IHC). UniProt describes broad expression with high heart levels; that tissue-level statement does not override the cell-specific IHC observation (UniProt O14737; HPA tissue IHC). |
| Subcellular interpretation | Cytosol is the approved main IF location, with nucleoplasm approved additionally; UniProt reports nuclear accumulation after genotoxic stress (HPA subcellular; UniProt O14737). Record cytoplasmic and nuclear staining separately when scoring, and interpret nuclear predominance in its experimental context (general IHC practice). |
| Antibody evidence and limits | The listed antibody HPA018471 has Enhanced IHC validation, while the tissue summary reports medium consistency with RNA and disregarded presumed off-target binding (HPA antibodies; HPA tissue IHC). Validation supports the reference pattern but does not make every stained cell or compartment specific (HPA tissue IHC). |
| Molecular features | PDCD5 has no annotated transmembrane segment or signal peptide and has two listed isoforms (UniProt O14737). These annotations support caution about a membrane-only interpretation, but the supplied record gives no isoform-specific staining pattern or epitope location (UniProt O14737); do not infer one. |
| IF/ICC Q: What localisation should be expected? | A: Mainly cytosolic signal with additional nucleoplasmic signal; HPA approves both locations and lists A-431, U-251MG and U2OS among cell lines with ICC-IF images (HPA subcellular). This is a localisation reference for the separate IF/ICC guide, not a prediction of relative intensity in a new sample (HPA subcellular). |
| Situation | Likely cause | Next action |
|---|---|---|
| Expected high-staining cells are present, but the IHC slide is blank. | The result conflicts with HPA's high staining in placental cytotrophoblasts, skin endothelial cells or late spermatids; the supplied sources do not identify a PDCD5-specific failure mechanism (HPA tissue IHC). | Run a known-positive section alongside the sample and check antibody incubation, retrieval, detector and chromogen steps one at a time (general IHC practice). Interpret a negative specimen only after the control gives interpretable cellular staining (general IHC practice). |
| The whole section has brown haze or staining beyond cell boundaries. | Widespread colour is inconsistent with the reported cellular cytoplasmic pattern (HPA tissue IHC). Background may reflect incomplete blocking, residual detection activity or excess reagent in a chromogenic workflow (general IHC practice). | Compare a no-primary control; review blocking, washes and chromogen development, then rescore only discrete staining in identifiable cells (general IHC practice). Do not use background colour to assign PDCD5-positive cell populations (HPA tissue IHC; general IHC practice). |
| A sharp membrane outline dominates the signal. | That localisation conflicts with cytosolic predominance and the absence of a transmembrane segment (HPA subcellular; UniProt O14737 topology). | Inspect an expected high-staining control and a no-primary control, then check whether intracellular cytoplasmic staining appears under controlled detection conditions (HPA tissue IHC; general IHC practice). Treat a persistent membrane-only pattern as unverified (HPA subcellular; UniProt O14737 topology). |
| Nuclear staining exceeds cytoplasmic staining. | Nucleoplasmic localisation is supported, and UniProt reports nuclear accumulation after genotoxic stress; nuclear predominance alone does not establish a technical failure (HPA subcellular; UniProt O14737). | Score nuclear and cytoplasmic compartments separately, compare matched controls and document any genotoxic treatment (general IHC practice; UniProt O14737). Investigate if the pattern is confined to implausible cells or accompanies diffuse background (HPA tissue IHC; general IHC practice). |
| Strong staining appears in HPA-listed negative cells. | HPA reports no detection in examples including cardiomyocytes and prostate glandular cells, and its tissue assessment acknowledges presumed off-target binding (HPA tissue IHC). | Confirm cell identity on the counterstained section and compare positive and no-primary controls (general IHC practice). If the unexpected signal persists, describe it as discordant with the HPA reference instead of assigning PDCD5 expression from colour alone (HPA tissue IHC). |
| An apparently negative heart section conflicts with UniProt's high heart expression. | UniProt gives a tissue-level expression summary, whereas HPA specifically reports cardiomyocytes as not detected by tissue IHC (UniProt O14737; HPA tissue IHC). | Identify which heart cell population is being scored and use a HPA high-staining cell population as the positive reference (HPA tissue IHC; general IHC practice). Report the cell-specific observation without converting the UniProt tissue summary into an expected cardiomyocyte stain (UniProt O14737; HPA tissue IHC). |
Experiment evidence
IHC & IF images showing PDCD5 staining
A02613-1 · IHCIHC analysis of PDCD5 using anti-PDCD5 antibody (A02613-1). PDCD5 was detected in a paraffin-embedded section of human stomach tissue. Heat
A02516-2 · IFImmunocytochemistry of PDCD5 in Jurkat cells with PDCD5 antibody at 5 μg/mL.
A02613 · IFImmunocytochemistry of PDCD5 in Jurkat cells with PDCD5 antibody at 2 μg/mL.
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000105185-PDCD5/tissue/cerebral+cortex#imid_5133380
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000105185-PDCD5/tissue/colon#imid_5133314
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000105185-PDCD5/tissue/liver#imid_5133307
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000105185-PDCD5/tissue/kidney#imid_5133300
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA018471; green = PDCD5, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA018471; green = PDCD5, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for PDCD5 IHC & IF
🧪Run placenta first and score cytotrophoblasts, which show High PDCD5 staining (HPA: placenta, cytotrophoblasts High). Use cervix glandular cells as a tissue negative (HPA: cervix, glandular cells Not detected); on the placenta slide, cells scored as internal negatives should show counterstain without brown DAB signal.
Positive control tissue: Placenta (Cytotrophoblasts, HPA High)
Negative control tissue: Caudate (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show PDCD5 in A-431, U-251MG, U2OS, with annotated localisation: Cytosol (approved) (HPA subcellular).
Technical controls: Include a no-primary (secondary-only) control and an isotype control using rabbit IgG matched to the primary antibody’s concentration (IHC caption: rabbit anti-PDCD5, 2 μg/ml). Use a PDCD5 knockout specimen processed alongside the test tissue as a biological negative; quench endogenous peroxidase and assess background in blood-rich placental areas before interpreting DAB staining (HPA: placenta; IHC caption: HRP/DAB detection).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected A02613-1 tissue-IHC caption does not state the fixative (IHC caption: fixative not stated). Heat retrieval in EDTA at pH 8.0 produced staining in a paraffin stomach section, but the evidence does not establish that retrieval is required or that frozen sections or IF/ICC are easier (IHC caption: stomach section and EDTA retrieval; HPA: ICC-IF images in A-431, U-251MG and U2OS). In placenta, blood-associated endogenous peroxidase can complicate interpretation of chromogenic signal, so assess the no-primary slide for background (HPA: placenta; IHC caption: HRP/DAB detection).
HPA tissue IHC evidence for PDCD5
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — Medium consistency between antibody staining and RNA expression data. Presumed off target binding observed and disregarded.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Placenta | Cytotrophoblasts | High | Protein (IHC) | HPA → |
| Skin | Endothelial cells | High | Protein (IHC) | HPA → |
| Testis | Elongated or late spermatids | High | Protein (IHC) | HPA → |
| Adrenal gland | Glandular cells | Medium | Protein (IHC) | HPA → |
| Appendix | Glandular cells | Medium | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Caudate | Glial cells | Not detected | Protein (IHC) | HPA → |
| Cerebellum | Cells in granular layer | Not detected | Protein (IHC) | HPA → |
| Cervix | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Epididymis | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Heart muscle | Cardiomyocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced PDCD5 IHC Tips
Troubleshoot PDCD5 staining in paraffin sections by checking retrieval, staining controls, cellular location, and scoring before interpreting biological differences.
What retrieval conditions should I try first for weak PDCD5 staining?
Start with heat-mediated retrieval in EDTA at pH 8.0 for paraffin sections (datasheet A02613-1). The selected stomach-section image used this retrieval before 10% goat-serum blocking, 2 μg/ml primary antibody overnight at 4°C, and DAB development (datasheet A02613-1). If staining is weak, check deparaffinisation, section adherence, and a positive control, then vary heating time incrementally while keeping EDTA at pH 8.0 (standard IHC practice; datasheet A02613-1). Develop adjacent sections for the same DAB time, assess tissue damage and background, and compare staining with the expected predominantly cytosolic location (standard IHC practice; HPA subcellular).
Could fixation explain inconsistent PDCD5 staining between paraffin samples?
Target-specific fixation sensitivity for PDCD5 is unknown because the selected paraffin-section caption does not report a fixative (datasheet A02613-1). Record fixative, time to fixation, fixation duration, and section age, then compare matched specimens using EDTA retrieval at pH 8.0 (standard IHC practice; datasheet A02613-1). Keep primary incubation consistent with the reported 2 μg/ml overnight at 4°C while evaluating morphology and staining (datasheet A02613-1). Treat a processing-related explanation as provisional until matched sections reproduce the difference; tissue expression, topology, and modification annotations do not establish a PDCD5-specific fixation effect (standard IHC practice; UniProt O14737).
How should I assess nuclear PDCD5 staining in chromogenic sections?
Assess cytoplasmic and nuclear staining separately: tissue IHC shows a ubiquitous cytoplasmic profile, while subcellular imaging places PDCD5 mainly in the cytosol with additional nucleoplasmic localisation (HPA tissue IHC; HPA subcellular). Nuclear accumulation can occur after genotoxic stress, so a nuclear pattern needs biological context and a control for processing artefacts (UniProt O14737; standard IHC practice). Compare matched sections retrieved in EDTA at pH 8.0 and developed for equal DAB times, recording staining intensity in each compartment (datasheet A02613-1; standard IHC practice). Exclude poorly preserved cells and section edges before interpreting a compartment shift, and avoid treating nuclear signal alone as proof of DNA damage (standard IHC practice; UniProt O14737).
Can epitope or isoform differences explain variable PDCD5 staining?
PDCD5 has 2 annotated isoforms, but the supplied antibody caption does not identify its epitope or establish isoform recognition (UniProt O14737; datasheet A02613-1). The protein has no annotated transmembrane segment, and reported modifications include phosphorylation at Ser-51 and Ser-119 and acetylation at Lys-63 (UniProt O14737). These annotations do not show whether fixation, retrieval, or modification changes binding by this antibody, so treat any proposed epitope effect as a hypothesis (UniProt O14737; standard IHC practice). Compare serial sections under the reported EDTA pH 8.0 retrieval and a consistent primary concentration of 2 μg/ml before attributing differences to isoforms (datasheet A02613-1; standard IHC practice).
How can IF help resolve ambiguous PDCD5 localisation seen by IHC?
Use IF/ICC as a complementary localisation check: imaging places PDCD5 mainly in the cytosol and additionally in the nucleoplasm (HPA subcellular). For multiplexing, pair PDCD5 with an independently validated marker of the expected cell type, such as a cytotrophoblast marker when examining placenta, where cytotrophoblast staining is high (HPA tissue IHC; standard IF practice). Choose a spectrally separated, preferably far-red fluorophore when tissue autofluorescence overlaps shorter wavelengths, and include single-label controls (standard IF practice). Because the target is intracellular and has no transmembrane segment, assess permeabilisation for access to cytosolic and nuclear epitopes; the IHC caption supplies no IF fixation conditions (UniProt O14737; standard IF practice; datasheet A02613-1).
What should I check when PDCD5 DAB staining is diffuse or patchy?
Compare staining with a no-primary control and inspect whether deposit follows cells, tissue edges, damaged areas, or endogenous pigment (standard IHC practice). The reported stomach-section workflow used 10% goat-serum blocking, 2 μg/ml primary antibody overnight at 4°C, and DAB detection (datasheet A02613-1). Apply a peroxidase block and standardise DAB development when troubleshooting chromogenic background; these are general workflow controls, not evidence of PDCD5 specificity (standard IHC practice). Evaluate candidate signal against the predominantly cytosolic pattern and remember that presumed off-target tissue staining was observed and disregarded in the HPA assessment (HPA subcellular; HPA tissue IHC).
How should I quantify PDCD5 staining across differently composed sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and compartment before scoring, because PDCD5 is mainly cytosolic but can also appear in the nucleoplasm (HPA subcellular). For a defined cell population, report percent positive cells and an H-score from 0–300 using staining intensity; score nuclear and cytoplasmic signal separately when relevant (standard IHC practice; HPA subcellular). For sparse populations, report positive-cell density per mm² of viable tissue and normalise comparisons to the same cell type, tissue area, and staining run (standard IHC practice). Use matched retrieval at EDTA pH 8.0, fixed DAB development, and consistent exclusion of necrosis and edges before comparing specimens (datasheet A02613-1; standard IHC practice).
How can I distinguish convincing PDCD5 positivity from an artefact?
Seek reproducible cellular staining with an appropriate compartment pattern: tissue IHC is broadly cytoplasmic, with high staining reported in placental cytotrophoblasts (HPA tissue IHC). A diffuse extracellular deposit, isolated edge staining, or signal confined to necrotic regions warrants review against adjacent sections and no-primary controls (standard IHC practice). Check endogenous peroxidase activity before accepting DAB signal, and question a solely nuclear pattern without supporting context, although PDCD5 can accumulate in nuclei after genotoxic stress (standard IHC practice; UniProt O14737). Interpret cell-type comparisons cautiously: HPA reports no detection in caudate glial cells and notes presumed off-target staining in its tissue assessment (HPA tissue IHC).
Boster reagents
Best PDCD5 / Programmed cell death protein 5 IHC Antibodies
The catalog shows PDCD5 IHC in human paraffin stomach sections and ICC/IF in Jurkat cells (catalog image captions); mouse and rat reactivity is listed for the cell-imaging antibodies (catalog reactivity).
Real IHC data
Anti-PDCD5 Antibody
Cat # A02613-1
Real IF data
Anti-PDCD5 Antibody
Cat # A02516-2
Real IF data
Anti-PDCD5 Antibody
Cat # A02613
A02613-1 has an IHC image from a human paraffin stomach section (A02613-1 image caption). A02516-2 has a Jurkat ICC image at 5 μg/mL, while A02613 has Jurkat ICC and IF images at 2 μg/mL and 10 μg/mL, respectively (catalog image captions).
Which to pick: Choose A02613-1 for human tissue IHC: it is listed as polyclonal and IHC-reactive, and its image documents a paraffin stomach section with EDTA retrieval; the fixative is unreported (A02613-1 catalog entry and image caption). Choose A02516-2 for ICC or A02613 when IF is needed, based on their application lists and Jurkat images (catalog entries and image captions). For mouse or rat samples, A02516-2 and A02613 list those species as reactive, but the supplied imaging examples are in Jurkat cells, so confirm staining in the intended species (catalog reactivity and image captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry O14737 (PDCD5_HUMAN, Programmed cell death protein 5).
- Human Protein Atlas. PDCD5 tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. PDCD5 subcellular location (ICC-IF): Mainly localized to the cytosol. In addition localized to the nucleoplasm..
- Human Protein Atlas. PDCD5 antibody validation summary (1 antibodies).
- The reduced PDCD5 protein is correlated with the degree of tumor differentiation in endometrioid endometrial carcinoma. SpringerPlus 2016 — PMC4937001.
- Pretreatment of mice with oligonucleotide prop5 protects them from influenza virus infections. Viruses 2014 — PMC3939472.
- PDCD5 Contributes to Airway Epithelial Cell Damage via Mitochondrial Pathway and Participates in COPD Pathogenesis. The Kaohsiung journal of medical sciences 2026 — PMC13344223.
- Low programmed cell death 5 expression is a prognostic factor in ovarian cancer. Chinese medical journal 2015 — PMC4832950.
- PubMed PMID:9920759 — UniProt-cited evidence.
- PubMed PMID:14702039 — UniProt-cited evidence.
- PubMed PMID:15057824 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for PDCD5 (Programmed cell death protein 5). Expected localisation Cytoplasm in tissue sections (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet A02613-1). Validated IHC antibody: A02613-1 at 1:50 recommended; image 2 μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Placenta (Cytotrophoblasts); negative Caudate. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human stomach tissue; fixative not specified (datasheet A02613-1); 2. Fixation: Image fixative and duration unreported (datasheet A02613-1); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet A02613-1); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet A02613-1); 8. Primary antibody: Rabbit anti-PDCD5, 1:50 recommended; image 2 μg/ml (datasheet A02613-1); 9. Primary incubation: Overnight at 4 °C (datasheet A02613-1). Watch for: Fixation: Keep fixation consistent across sections (standard IHC practice; not target-specific); Caveat: Presumed off-target staining may mimic PDCD5 (HPA tissue IHC); Isoform / epitope: 2 isoforms; epitope impact unspecified (UniProt); Regulation: Genotoxic stress: nuclear accumulation (UniProt). Evidence: UniProt O14737, HPA tissue IHC · Enhanced, HPA ICC-IF · Mainly localized to the cytosol. In addition localized to the…, Datasheet A02613-1, PMC4937001, PMC3939472. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/PDCD5/immunohistochemistry-ihc-guide.