- Table of Contents
We validate the specificity of these antibodies to 40S ribosomal protein S6 by testing them on tissues known to express RPS6 positively and negatively. Browse below to find the RPS6 antibody that suites your experiment. We have 8 of these antibodies and many publications and validation images.
If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.
Facts about 40S ribosomal protein S6.
|eukaryotic ribosomal protein eS6 family|
Phosphoprotein NP33, 40S ribosomal protein S6; pS6; Ribosomal Protein S6; RPS6; S6
|Sequence:||9; NC_000009.12 (19375715..19380236, complement)|
This Boster Bio product guide features a stepwise IHC workflow with illustrations. The Boster Bio IHC protocol also includes troubleshooting guides and optimization tips. Read on for more. Listed below are some of the best Boster Bio uses. These tips are applicable to all scientists around the world. Read on to discover what these tips are and how to apply them in your lab.
The Boster Bio guide focuses on IHC, a process that is both straightforward and highly effective, and it has the additional benefit of clearly illustrating the steps to achieve the best results. The guide includes a step-by-step overview of the whole procedure, including recommended reagents and sample preparation techniques. Preparation of tissues for IHC is one of the most critical steps in this process, as it determines the quality of the final staining.
The main challenge with IHC is achieving strong staining of the target antigens. Good staining requires the right quantity of primary and secondary antibodies, which should be matched for optimal results. Additionally, the primary antibody must bind to the secondary antibody properly to give the best results. Occasionally, weak signals occur and must be troubleshooted to avoid repeat experiments. To prevent weak signals, check the antibody datasheet and ensure that the sample is appropriate.
In this method, the tumor cells are cultured in a dish containing 5% FBS and 1% PMSF. Next, minced tumor tissue was lysed in Cell lysis buffer containing 1% PMSF. Next, proteins were denatured by heating at 100 degC for 10 minutes. Afterwards, the samples were transferred to a PVDF membrane coated with FFP28 or Beyotime. The membrane was then blocked with 5% skim milk and incubated for 2 h with primary antibodies of hPD-L1 or mPD-L1. The samples were then stained with secondary antibodies of hPD-L1 or GAPDH.
The authors acknowledge the support of the German Ministry of Education and the German Research Foundation. The authors report personal fees from various pharmaceutical companies. BK, LA, and KS are cofounders of Nocturne and have received speaker honoraria and travel reimbursement from Novartis and Biogen. RM has served on the scientific advisory boards of Viela Bio/Horizon Therapeutics. Both FP and NA have received research support from Biogen, Roche, and Diamed.
Among the authors, Dr. Nadeau has served as an FDA consultant and a member of NIH Study Sections. He has also served on the environmental health policy committee of the American Thoracic Society and the Data and Safety Monitoring Board of the National Heart, Lung, and Blood Institute. His other achievements include being a fellow of the American Academy of Allergy, Asthma, and Immunology. He is also the founder of the Gordon Research Conference on Food Allergy and was the first to chair the inaugural conference. He also serves on the Board of Scientific Counselors for the NIH Clinical Center.
The Boster Bio RPS6 marker immunohistochemistry troubleshooting guide includes a comprehensive list of useful IHC troubleshooting tips. The guide is broken down into sections covering common problems, such as weak staining, high background, nonspecific staining, and sample preparation. It also provides an overview of available immunohistochemistry resources. The guide is applicable to scientists worldwide, and includes helpful tips for every stage of the immunohistochemistry workflow.