Protein Wnt-2b Antibodies

Protein Wnt-2b (WNT2B)

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta- catenin signaling pathway.

Plays a redundant role in embryonic lung growth. .

Gene Information

Human
Gene Name:	WNT2B
Uniprot:	Q93097
Entrez:	7482

Family

Belongs to:
Wnt family

Alternative Names

Protein Wnt-13; protein Wnt-2b; wingless-type MMTV integration site family, member 2B; Wnt-13; WNT13XWNT2, Xenopus, homolog of; Wnt2b; Wnt-2b; XWNT2; XWNT2wingless-type MMTV integration site family, member 13

Molecular Weight

Mass (kDA):
43.77 kDA

Genomic Context

Human
Location:	1p13.2
Sequence:	1; NC_000001.11 (112466541..112530165)

Tissue Specificity

Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.

Subcellular Localizations

Secreted, extracellular space, extracellular matrix. Secreted.

Diseases

• Malignant Neoplasms
• Neoplasms
• Malignant Neoplasm Of Stomach
• Carcinogenesis
• Carcinoma
• Malignant Neoplasm Of Breast
• Stomach Neoplasms
• Embryonal Carcinoma
• Malignant Neoplasm Of Pancreas
• Neoplasm Metastasis
• Nervousness
• Hemorrhage
• Congenital Absence

• Basal Cell Carcinoma
• Malignant Neoplasm Of Lung
• Colorectal Cancer
• Malignant Tumor Of Cervix
• Cell Transformation, Neoplastic
• Leukemia
• Esophageal Neoplasms

Pathways

• Cell Proliferation
• Cell Differentiation
• Eye Development
• Glycosylation
• Localization
• Cell Growth
• Regeneration
• Cell Cycle
• Gastrulation
• Limb Development
• Fermentation
• Cell Motility
• Organ Development

• Mesoderm Development
• Pathogenesis
• Regionalization
• Rna Splicing
• Tissue Regeneration
• Kidney Development
• Cell Migration

PTMs

• Glycosylation

• Phosphorylation

Research Areas

• Stem Cell Signaling Pathway
• Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/WNT2B

Best Uses Of The WNT2B Marker

You've found the right place if you're looking for a method to detect WNT2B. We have information about Anti-ProteinWnt2B, clinical uses, and the MiR-137 inhibitory effects of Wnt2B. Next, we'll go over luciferase reporter assay, which is used to measure WNT2B levels in cell lines.

Anti-Protein Wnt-2b

The WNT2B deficiency gene is a key player in regulating the intestinal microbiota. Deficient enteroids exhibit osmotic diarrhea and a reduced ability to thrive. The integrity of the intestinal microbiota is affected by a WNT2B defect. This is due to impaired generation and maintenance ISC. It is also implicated in the dysregulation of host-microbial pattern recognition signaling.

The Wnt pathway, a conserved signaling mechanism that regulates many physiological as well as pathological processes, is important. HCC patients are affected by an abnormal activation the Wnt/b/catenin pathway. This pathway has been implicated in the progression of the condition. Wnt signaling, in addition to mediating cell to cell interactions, has been shown to increase glycolysis of tumour cells. Wnt3a promotes the polarization of M2 and activates b–catenin in macrophages.

WNT/b–catenin signaling regulates cell function during embryonic growth and adulthood. Studies have shown that elevated levels of this protein can lead to abnormal development, as well as various disease states. Evidence is growing that WNT signals play a crucial role in tissue growth and repair. WNT signaling is context dependent, and WNT activation is associated to an increase in the risk of several conditions.

Wnt2b regulates the Wnt signals in the eye. Wnt2b activity can be detected in peripheral OC as well as RPE. It was also detected in differentiated retinal cells such as rPE and iris cells. These pathways are crucial in controlling eye development. This pathway also regulates other signaling paths in the eye.

A recent study examined the role played by WNT signaling on the kidney's perivascular cells. Wnt/betacatenin signals regulate the migration of hepatic Stellate cells. The CTNND1 gene promoted hepatocellular carcinoma cell migration and stimulated WNT secretion, according to the researchers. IWP2 blocks WNT production and activates the MAPK pathways within macrophages.

MiR-137 inhibitory effect on Wnt2b

Recent research has shown that miR137 can inhibit WNT2B activity through inhibiting DNMT3A. Overexpressions miR-137 suppress the PTEN/Akt pathway and inhibit HCC cell growth. WNT2B activity is not inhibited by miR137 alone. Inhibitory effects of miR-137 on WNT2B have been associated with a reduction in WNT2B-dependent apoptosis.

To determine whether miR137 has an inhibitory affect on WNT2B activities, we first determined its binding to the WNT2B Gene. MiR-137 has high affinity for WNT2B, which is expressed on the pancreatic cell line CCA. MiR-137 inhibits WNT2B protein expression and suppresses cell proliferation, migration, or invasion.

Cholangiocarcinoma is caused by miR-137 binding to WNT2B mRNA 3'-UTR and inhibiting luciferase activity. Interestingly, the mimic effect was lost when the tumor cells were overexpressed with miR-137. Additionally, the expression of miR-137 was inversely associated with WNT2B mRNA in cholangiocarcinoma tissue tissues.

In another study, we examined the role of miR137 in WNT2B regulation. Co-transfection in 293T cells of wild-type or mutant plasmids reversed the inhibitory action of miR137. Similar results were observed in the miR137-transfected cells. They had lower levels of LRP6 and b-catenin.

Additionally, miR137 inhibits GBM cell growth and invasion. Inhibition of miR-137 by LRP6 partially reverses the inhibitory effect of miR-137 on WNT2B. These studies show that miR137 inhibits WNT2B activity for a variety of tumors including GBM. The presence of LRP6 at the cell surface is responsible for miR-137's inhibitory effect on WNT2B.

Clinical applications

There are many uses for WNT2B in clinical settings. This protein was shown to increase the rate at which fibroblast activation occurs in TMEs. The protein is found in exosomes produced by CC cells. These exosomes have a role in tumor initiation and progression. The WNT2B gene marker has potential to be used in cancer research and treatment.

The HCC-TCM induces polarization through the WNT2B gene. Expression of WNT2B in the bloodstream increases E-cadherin/N-cadherin ratios. This study suggests that WNT2B might be involved in HCC/TAM-induced H2 polarization. This could have therapeutic implications in cancer research.

WNT2B expression was found to be high in human sporadic carcinoma cells. Wnt2B expression was detected cytoplasmatically and nuclearly. Translocations into the nucleus of b-catenin showed activation in Wnt signaling. In tumors, palmitoylation increased. This marker may be useful in diagnosing colorectal cancer.

The TSK molecule can be used to inhibit WNT2b activitiy. TSK binds to the cell's Fzd4 signal and inhibits WNT2b activity. TSK is a promising therapeutic option for WNT2B inhibitors. CpG–ODN is a TSK inhibitor that can inhibit Wnt2b and may be useful in clinical cancer therapy. This inhibitor could be used in HCC–TAMs to treat the WNT2b receptor.

The WNT2B genome was transfected into T cells 293 times. The lentiviruses were cotransfected with the pLKO.1–sh-Wnt2b knockdown expression and pMD2G. Cells were treated after transfection with DMSO or GW4869. The cells expressed Wnt2B. They also displayed Wnt2b signals.

luciferase reporter assay

A luciferase researcher assay is a reagentbased approach to understanding how a protein regulates transcript. The reporter protein is used to produce the luciferase enzyme. This enzyme is released by cells when the target proteins activate transcription. This activity is measured by the luminometer using a special reagent. There are several luciferase reporter-assay reagents that you can choose from, including QuantiLum r (recombinantluciferase) by Boster Bio.

T cells with luciferase gene expression are used in reporter assays. The fluorescent protein is introduced into the cells via retroviral or transient methods. Luciferase-transgenic mouse strains are developed using similar methods. They require longer development times, but they don't require any further modifications to the original mouse strain. Furthermore, they can be used to study cells in their natural state.

Dual-luciferase assay was performed using human 293T cells. It was discovered that co-transfection with Wnt3a's 3'UTR mutation of luciferase reporter significantly increased activity. This experiment was conducted using human 293T cell lines to test the role played by Wnt3a on controlling cell growth. This assay also revealed that luciferase activity was directly associated with Wnt3a expression in rat BMSCs.

Dual reporters are available for the reporter assay for Luciferase: Fluc and Nluc. The Nluc reporter has the highest signal and can be used for short-term non-lytic analyses. The latter is better suited for long-term kinetic analyses, and has the greatest stability. This two-part series also covers lysate preparation, delivery and storage methods. Part two discusses data analysis, experimental design, and other topics.

Boster Bio provided the Human High Sensitivity ELISA Kit. The assay detects inflammatory cytokines (IL-6 and IL-1b) in different cell line types. The HUVEC cells were sown into 96-well plates to perform this assay. The cell mediums then were analyzed with qRT-PCR.

High-affinity primary antibodies

A WNT2B mark is a very efficient way to make high-affinity prima antibodies. The marker can be found in cells of the immune systems. The WNT2B protein molecule recognizes a specific protein and binds to it, such as a human receptor for T cells. The WNT2B protein was used in this study to identify high-affinity IgM antibodies and IgG antibodies.

An affinity-maturation test was performed to measure IgM affinities. We used either the InsA(4) plates or InsA(1) plates. The IgM affinity of each day was determined by dividing the two by their respective numbers. We then used streptavidins with tetravalent or monovalent binding capacities and the results are expressed as relative units. A high-affinity, primary antibody using WNT2B markers is a promising candidate to develop drugs in cancer immunology.

The WNT2B is a ligand that the cytokine IgD receptor. It regulates the release of antigen-specific IgG antibody and controls the activation in B cells. This activity is blocked by a deficient WNT2B gene in the cell membrane. This is a promising marker for the development of therapeutic antibodies in autoimmune diseases.

Aves Labs offers antibodies at two levels. The company is located in Davis, CA and develops novel antibody reagents against epitope tags and neural-specific antigens. Aves Labs produces high-affinity antibodies to help advance neurobiology research. Its antibodies are produced at two levels, allowing researchers to analyze both the WNT2B gene marker and the WNT2B-protein in their own labs.