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Product Info Summary
| SKU: | A04879-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Wnt2b Antibody Picoband®
SKU/Catalog Number
A04879-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Wnt2b Antibody Picoband® catalog # A04879-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wnt2b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04879-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Wnt2b recombinant protein (Position: A7-T391).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04879-1 is reactive to WNT2B in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
43.8 kDa
Background of WNT2B
WNT2B (Wingless-Type MMTV Integration Site Family Member 2B), is a protein that in humans is encoded by the WNT2B gene. This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. Katoh et al. (1996) used fluorescence in situ hybridization to map the WNT13 gene to chromosome 1p13. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04879-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HEK293 whole cell, human HEPG2 whole cell, human Jurkat whole cell, human A549 whole cell, human Raji whole cell, human SW620 whole cell, human SKOV3 whole cell, rat PC-12 whole cell, mouse Neuro-2a whole cell, mouse ANA-1 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Wnt2b using anti-Wnt2b antibody (A04879-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human HEPG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: human SW620 whole cell lysates,
Lane 7: human SKOV3 whole cell lysates,
Lane 8: rat PC-12 whole cell lysates,
Lane 9: mouse Neuro-2a whole cell lysates,
Lane 10: mouse ANA-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt2b antigen affinity purified polyclonal antibody (Catalog # A04879-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt2b at approximately 60KD. The expected band size for Wnt2b is at 60KD.
Specific Publications For Anti-Wnt2b Antibody Picoband® (A04879-1)
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