Anti-Protein Wnt-2b Wnt2b Antibody

Boster Bio Anti-Protein Wnt-2b Wnt2b Antibody catalog # PA1985. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA1985
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Protein Wnt-2b Wnt2b Antibody

See all Wnt-2b products

SKU/Catalog Number

PA1985

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Protein Wnt-2b Wnt2b Antibody catalog # PA1985. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Protein Wnt-2b Wnt2b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1985)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Wnt2b (169-185aa DPYTRGRHHDQRGDFDW), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1985 is reactive to WNT2B in Human, Mouse, Rat

Applications

PA1985 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For WNT2B (Source: Uniprot.Org, NCBI)

Uniprot ID

Q93097

Gene ID

7482

Gene Name

WNT2B

Full Name

Protein Wnt-2b

Weight

43.77kDa

Superfamily

Wnt family

Alternative Names

Protein Wnt-13; protein Wnt-2b; wingless-type MMTV integration site family, member 2B; Wnt-13; WNT13XWNT2, Xenopus, homolog of; Wnt2b; Wnt-2b; XWNT2; XWNT2wingless-type MMTV integration site family, member 13 WNT2B WNT13 Wnt family member 2B protein Wnt-2b|XWNT2, Xenopus, homolog of|wingless-type MMTV integration site family, member 13|wingless-type MMTV integration site family, member 2B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on WNT2B, check out the WNT2B Infographic

WNT2B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for WNT2B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1985 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

An R,Wang X,Yang L,Zhang J,Wang N,Xu F,Hou Y,Zhang H,Zhang L. Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats.Toxicology.2020 Dec 24;449:152665. doi:10.1016/j.tox.2020.152665.Epub ahead of p
Species: Rat
PA1985 usage in article: APP:WB, SAMPLE:OVARY TISSUE, DILUTION:1:500

Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
PA1985 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTIO N:NA

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4 Customer Q&As for Anti-Protein Wnt-2b Wnt2b Antibody

Question

Would anti-Wnt2b antibody PA1985 work for WB with milk?

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

According to the expression profile of milk, WNT2B is highly expressed in milk. So, it is likely that anti-Wnt2b antibody PA1985 will work for WB with milk.

Boster Scientific Support

Answered: 2020-04-21

Question

Will PA1985 anti-Wnt2b antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

It shows on the product datasheet, PA1985 anti-Wnt2b antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-03

Question

Is this PA1985 anti-Wnt2b antibody reactive to the isotypes of WNT2B?

Verified Customer

Verified customer

Asked: 2017-11-24

Answer

The immunogen of PA1985 anti-Wnt2b antibody is A synthetic peptide corresponding to a sequence in the middle region of human Wnt2b(169-185aa DPYTRGRHHDQRGDFDW), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-24

Question

Please see the WB image, lot number and protocol we used for milk using anti-Wnt2b antibody PA1985. Please let me know if you require anything else.

D. Johnson

Verified customer

Asked: 2015-06-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-06-02

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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PA1985
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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