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- Table of Contents
WNT2B antibody (PA1985) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Anti-Wnt2b antibody, PA1985, Western blotting
Recombinant Protein Detection Source :E.coli derived -recombinant Human WNT2B, 35.78KD (162aa tag+ R101-A254)
Lane 1: Recombinant Human WNT2B Protein 10ng
Lane 2: Recombinant Human WNT2B Protein 5ng
Lane 3: Recombinant Human WNT2B Protein 2.5ng
Recombinant Protein Detection Source :E.coli derived -recombinant Human WNT2B, 35.78KD (162aa tag+ R101-A254)
Lane 1: Recombinant Human WNT2B Protein 10ng
Lane 2: Recombinant Human WNT2B Protein 5ng
Lane 3: Recombinant Human WNT2B Protein 2.5ng
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Anti-Protein Wnt-2b Wnt2b Antibody Picoband®
WNT2B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
WNT2B antibody was positively detected by WB in:
recombinant human WNT2B protein
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Protein Wnt-2b Wnt2b Antibody Picoband®
View all WNT2B Antibodies
View all WNT2B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Wnt2b, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
450 kDa
Calculated Molecular Weight
43.8 kDa
Background of WNT2B
WNT2B (Wingless-Type MMTV Integration Site Family Member 2B), is a protein that in humans is encoded by the WNT2B gene. This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. Katoh et al. (1996) used fluorescence in situ hybridization to map the WNT13 gene to chromosome 1p13. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis.
Cite This Product
Anti-Protein Wnt-2b Wnt2b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1985)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
65%
Low homology
Drosophila
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for WNT2B antibody
Publications
Application:
Species:
An R,Wang X,Yang L,Zhang J,Wang N,Xu F,Hou Y,Zhang H,Zhang L. Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats.Toxicology.2020 Dec 24;449:152665. doi:10.1016/j.tox.2020.152665.Epub ahead of p
Species: Rat
PMID: 33359712
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
PMID: 32463570
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Product Promise
We promise Anti-Protein Wnt-2b Wnt2b Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PA1985
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026