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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
Boster offers a full range of lab services for immunohistochemistry (IHC) and immunofluorescence (IF). Get a free consultation today.
Want to have all technical references by your finger tips? Download our FREE eBooks for WB, IHC, ELISA, Flow cytometry and Molecular biology here. These ebooks contain detailed information regarding the principles, protocols troubleshooting tips and optimization tips for their respective assays. Handy for newbies and veterans alike.
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Weak signal is typically caused by problems in the blocking or washing steps, but can also be caused by a number of other issues. Read our tips to identify and troubleshoot your weak signal results.
Keywords: Weak Signal, Low Signal, Western Blot Troubleshooting
Western blotting separates proteins based on size; large proteins migrate through a polyacrylamide gel matrix slower than small proteins do. However, other factors can influence the migration rate of proteins, resulting in band sizes different than predicted based on protein size alone.
Keywords: Band Size, Wrong Band Size, Western Blot Troubleshooting
Many Western blot problems arise due to issues with the background. Splotches, streaks, or background staining can ruin results. Use our troubleshooting tips to identify and resolve the cause of your background troubles.
Keywords: Flecked Background, Speckled Background, Blotched Background, Western Blot Troubleshooting
High background on a western blot occurs when the background signal of the membrane reduces the signal-to-noise ratio to unreadable levels. Learn our troubleshooting tips to identify and resolve the cause of your high background:
Keywords: High Background, Saturated Background, Background Staining, Nonspecific Staining, Western Blot Troubleshooting
Distorted bands can make it very hard to interpret your results. Common distortions include smile shaped bands with the edges trailing upward, diffuse bands that are broad or blurry, and streaked bands that trail off in several directions. Make sure your next blot has even, crisp bands by reading our tips.
Keywords: Smile Bands, Distorted Bands, Uneven Bands, Western Blot Troubleshooting
High background in IHC occurs when the contrast between the antibody stain and the background staining is low, resulting in poor data. Use our tips to identify and troubleshoot the cause of your high background:
Keywords: High Background, IHC Troubleshooting
Many conditions affect the outcome of IHC staining. In order to maximize your results, proper identification and troubleshooting of issues is key. Read some tips to help you improve your IHC staining.
Keywords: Low Signal, Weak Signal, Low Staining, IHC Troubleshooting
Overstaining can result in washed-out or oversaturated results, reducing the sharpness and clarity of a sample. Follow our suggestions for troubleshooting your overstained IHC experiment:
Keywords: Overstaining, Oversaturated Staining, IHC Troubleshooting
A variety of issues can arise during the staining step of IHC, such as non-specific staining. Non-specific staining occurs when the primary antibodies bind to proteins other than the target protein. Learn some tips to reduce non-specific binding when using IHC.
Keywords: Non-Specific Staining, High Background, IHC Troubleshooting
A number of problems commonly arise during ELSIA that can result in low signal and poor color development. Use are some troubleshooting tips to help you improve your experiment and get better data.
Keywords: Low Signal, Faint Color, ELISA Troubleshooting
Matrix Effect occurs when the target antigen interacts with matrix components in plasma or serum samples. These matrix components can be endogenous biological components such as phospholipids, carbohydrates, and metabolites. Matrix components can reduce the binding of the antibody to the target protein, or non-specifically bind the antibody, generating weak or noisy results. Read some tips to reduce matrix effect.
Keywords: Matrix Effect, ELISA Troubleshooting
On each 96 well plate, we recommend running an 8-point standard curve with duplicate wells. With the remaining 80 wells, 40 samples can be tested in duplicate. Learn more about the number of samples tested per micotiter plate.
Keywords: Number of Samples, Microtiter Plate, Maximum Usage, Setting Up Plate
ChIP sequencing (ChIP-Seq) is a powerful method for identifying genome-wide DNA binding sites for transcription factors and other proteins. Use this guide to help troubleshoot common issues that arise during ChiP procedures.
Keywords: ChIp, Seq, immunoprecipitation, Troubleshooting, Tips