ELISA: Troubleshooting Low Signal
A number of problems commonly arise during ELSIA that can result in low signal and poor color development. Use are some troubleshooting tips to help you improve your experiment and get better data.
Keywords: Low Signal, Faint Color, ELISA TroubleshootingLearn More About Low Signal
ELISA: Troubleshooting Matrix Effect
Matrix Effect occurs when the target antigen interacts with matrix components in plasma or serum samples. These matrix components can be endogenous biological components such as phospholipids, carbohydrates, and metabolites. Matrix components can reduce the binding of the antibody to the target protein, or non-specifically bind the antibody, generating weak or noisy results. Read some tips to reduce matrix effect.
Keywords: Matrix Effect, ELISA TroubleshootingLearn More About Matrix Effect
ELISA: How many samples can I run on a plate?
On each 96 well plate, we recommend running an 8-point standard curve with duplicate wells. With the remaining 80 wells, 40 samples can be tested in duplicate. Learn more about the number of samples tested per micotiter plate.
Keywords: Number of Samples, Microtiter Plate, Maximum Usage, Setting Up PlateLearn More About Number of Samples Per Plate
Western Blotting FAQs
Western Blot: Troubleshooting Weak Signal
Weak signal is typically caused by problems in the blocking or washing steps, but can also be caused by a number of other issues. Read our tips to identify and troubleshoot your weak signal results.
Keywords: Weak Signal, Low Signal, Western Blot TroubleshootingLearn More About Weak Signal
Western Blot: Troubleshooting Bands Wrong Molecular Weight
Western blotting separates proteins based on size; large proteins migrate through a polyacrylamide gel matrix slower than small proteins do. However, other factors can influence the migration rate of proteins, resulting in band sizes different than predicted based on protein size alone.
Keywords: Band Size, Wrong Band Size, Western Blot TroubleshootingLearn More About Band Size
Western Blot: Troubleshooting Background Background Blotchy, Flecked, Or Dirty
Many Western blot problems arise due to issues with the background. Splotches, streaks, or background staining can ruin results. Use our troubleshooting tips to identify and resolve the cause of your background troubles.
Keywords: Flecked Background, Speckled Background, Blotched Background, Western Blot TroubleshootingLearn More About Background
Western Blot: Troubleshooting High Background
High background on a western blot occurs when the background signal of the membrane reduces the signal-to-noise ratio to unreadable levels. Learn our troubleshooting tips to identify and resolve the cause of your high background:
Keywords: High Background, Saturated Background, Background Staining, Nonspecific Staining, Western Blot TroubleshootingLearn More About High Background
Western Blot: Troubleshooting Distorted Bands
Distorted bands can make it very hard to interpret your results. Common distortions include smile shaped bands with the edges trailing upward, diffuse bands that are broad or blurry, and streaked bands that trail off in several directions. Make sure your next blot has even, crisp bands by reading our tips.
Keywords: Smile Bands, Distorted Bands, Uneven Bands, Western Blot TroubleshootingLearn More About Band Distortion
IHC: Troubleshooting Weak Staining
Many conditions affect the outcome of IHC staining. In order to maximize your results, proper identification and troubleshooting of issues is key. Read some tips to help you improve your IHC staining.
Keywords: Low Signal, Weak Signal, Low Staining, IHC TroubleshootingLearn More About Weak Staining
IHC: Troubleshooting High Background
High background in IHC occurs when the contrast between the antibody stain and the background staining is low, resulting in poor data. Use our tips to identify and troubleshoot the cause of your high background:
Keywords: High Background, IHC TroubleshootingLearn More About High Background
IHC: Troubleshooting Overstaining
Overstaining can result in washed-out or oversaturated results, reducing the sharpness and clarity of a sample. Follow our suggestions for troubleshooting your overstained IHC experiment:
Keywords: Overstaining, Oversaturated Staining, IHC TroubleshootingLearn More About Overstaining
IHC: Non-specific Staining
A variety of issues can arise during the staining step of IHC, such as non-specific staining. Non-specific staining occurs when the primary antibodies bind to proteins other than the target protein. Learn some tips to reduce non-specific binding when using IHC.
Keywords: Non-Specific Staining, High Background, IHC TroubleshootingLearn More About Nonspecific Staining
ChIP Troubleshooting Guide
ChIP sequencing (ChIP-Seq) is a powerful method for identifying genome-wide DNA binding sites for transcription factors and other proteins. Use this guide to help troubleshoot common issues that arise during ChiP procedures.
Keywords: ChIp, Seq, immunoprecipitation, Troubleshooting, TipsLearn More About ChIp