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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA)|
|Assay Time:||90 Minutes|
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Human CES1 PicoKine® Quick ELISA Kit
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CES1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CES1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1 ng/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CES1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1813)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: G18-L567
0.78 ng/ml - 50 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
FEK1813 is reactive to CES1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
FEK1813 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Carboxylesterase 1/CES1
Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. This gene is mapped to 16q12.2. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.
|FEK1813-CAP||Anti-tag Pre-coated 96-well Strip Microplate||1||12 strips of 8 wells|
|FEK1813-ST||Human Carboxylesterase 1/CES1 Standard||2||50 ng/tube|
|FEK1813-C||Human CES1 Antibody Cocktail||1||6ml|
|AR1106-7||TBS-T Wash Buffer (25x)||1||12ml|
|AR1104||Color Developing Reagent (TMB)||1||10ml|
|PLA-SEA||Adhesive Plate Sealers||2||Piece|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CES1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Carboxylesterase 1/CES1 in ELISA kits from four different production batches/lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CES1 (Source: Uniprot.Org, NCBI)
Liver carboxylesterase 1
type-B carboxylesterase/lipase family
ACAT; acyl coenzyme A:cholesterol acyltransferase; Acyl-coenzyme A:cholesterol acyltransferase; Brain carboxylesterase hBr1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); Carboxylesterase 1; carboxylesterase 2 (liver); CEH; CES1; Ces-1; CES1A2; CES2EC 188.8.131.52; cholesteryl ester hydrolase; Cocaine carboxylesterase; EC 3.1.1; Egasyn; HMSE1; HMSECES1A1; human monocyte/macrophage serine esterase 1; liver carboxylesterase 1; MGC117365; Monocyte/macrophage serine esterase; PCE-1; REH; Retinyl ester hydrolase; Serine esterase 1; SES1; Ses-1; SES1TGH; TGH; Triacylglycerol hydrolase CES1 ACAT, CE-1, CEH, CES2, HMSE, HMSE1, PCE-1, REH, SES1, TGH, hCE-1 carboxylesterase 1 liver carboxylesterase 1|acyl coenzyme A:cholesterol acyltransferase|brain carboxylesterase hBr1|carboxylesterase 1 (monocyte/macrophage serine esterase 1)|carboxylesterase 2 (liver)|cholesteryl ester hydrolase|cocaine carboxylesterase|egasyn|human monocyte/macrophage serine esterase 1|methylumbelliferyl-acetate deacetylase 1|monocyte/macrophage serine esterase|retinyl ester hydrolase|serine esterase 1|triacylglycerol hydrolase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CES1, check out the CES1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CES1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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