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SKUAR1068
Size500 mL
ApplicationPreserving Samples, Fixing Cells For Histology
Physical StateThe solution should be clear, colorless, with no precipitate.

Overview

Product Name 4% Paraformaldehyde (PFA) Solution in PBS
SKU/Catalog Number AR1068
FormLiquid
Physical State The solution should be clear, colorless, with no precipitate.
Pack Size 500mL
Application Preserving Samples, Fixing Cells For Histology
*Our Boster Guarantee covers the use of this product in the above tested applications.
StorageStore at -20°C.
Safety and Handling Causes serious eye damage.
Suspected of causing cancer.
May cause an allergic skin reaction.
Causes skin irritation.
Avoid breathing dust/fume/gas/mist/vapors/spray.
Wear protective gloves/protective clothing/eye protection/face protection.
Use personal protective equipment as required.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Store locked up.
Dispose of contents/container in accordance with local/regional/national/international regulations.
Description 4% Paraformaldehyde solution in PBS is a ready to use solution used for sample preparation for fixing cells for immunohistochemistry (IHC).
Cite This Product 4% Paraformaldehyde (PFA) Solution in PBS (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1068)
Equivalent N/A

Properties

CAS Number30525-89-4
MDL NumberMFCD00133991
Solubility Soluble in water
pH 7.0 - 7.6 at 25°C

Background

Paraformaldehyde (PFA) is the polymerization product of formaldehyde with a typical degree of polymerization of 8–100 units. Paraformaldehyde is not a fixative itself; it must be depolymerized to formaldehyde in solution. In cell culture, a typical formaldehyde fixing procedure would involve using a 4% formaldehyde solution in phosphate buffered saline (PBS) on ice for 10 minutes. Fixing ensures that sample cell structures stay intact and that antigens are immobilized, while ideally still permitting unfettered access of antibodies to target antigens.

General Notes

• Adjust pH to 7.2-7.6 with NaH2PO4 if pH <7 after long storage.

• Deposition is normal for this product.

4% Paraformaldehyde (PFA) Solution in PBS Images

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4% Paraformaldehyde (PFA) Solution in PBS
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Publications

Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis
Long Non-Coding RNA GAPLINC Promotes Tumor-Like Biologic Behaviors of Fibroblast-Like Synoviocytes as MicroRNA Sponging in Rheumatoid Arthritis Patients
d-limonene exhibits antitumor activity by inducing autophagy and apoptosis in lung cancer
Regulation by FSH of the dynamic expression of retinol-binding protein 4 in the mouse ovary
Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury
The efficacy, biodistribution and safety of an inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis
Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity
Mechanism of apoptosis involved in gastric mucosal lesions in Tibetans with high-altitude polycythemia
Neuroprotective effect of total flavonoids from Ilex pubescens against focal cerebral ischemia/reperfusion injury in rats
Zonisamide-loaded triblock copolymer nanomicelles as a novel drug delivery system for the treatment of acute spinal cord injury
Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats
Influence of high-altitude hypoxic environments on the survival of cochlear hair cells and spiral ganglion neurons in rats
The citrus flavonoid naringenin confers protection in a murine endotoxaemia model through AMPK-ATF3-dependent negative regulation of the TLR4 signalling pathway
Knockout of Ccr2 alleviates photoreceptor cell death in rodent retina exposed to chronic blue light
MicroRNA-130a alleviates human coronary artery endothelial cell injury and inflammatory responses by targeting PTEN via activating PI3K/Akt/eNOS signaling pathway
Jin Fu Kang Oral Liquid Inhibits Lymphatic Endothelial Cells Formation and Migration
Sanguisorba officinalis L synergistically enhanced 5-fluorouracil cytotoxicity in colorectal cancer cells by promoting a reactive oxygen species-mediated, mitochondria-caspase-dependent apoptotic pathway
Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1
In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material
EZH2 is overexpressed in laryngeal squamous cell carcinoma and enhances the stem-like properties of AMC-HN-8 cells
LmCYP4G102: An oenocyte-specific cytochrome P450 gene required for cuticular waterproofing in the migratory locust, Locusta migratoria
Effects of fluoride on proliferation and mineralization in periodontal ligament?cells?in vitro
Microvesicles derived from hypoxia/reoxygenation-treated human umbilical vein endothelial cells promote apoptosis and oxidative stress in H9c2 cardiomyocytes
Differential expression of androgen, estrogen, and progesterone receptors in benign prostatic hyperplasia
Right coronary artery fistula misdiagnosed as right atrial cardiac myxoma: A case report
Shox2 influences mesenchymal stem cell fate in a co-culture model in?vitro
Astragalus membranaceus?ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition?in vivo?and?in vitro
Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing
Electric pulse current stimulation increases electrophysiological properties of If?current reconstructed in mHCN4-transfected canine mesenchymal stem cells
Expression and clinical significance of Beclin-1 in gastric cancer tissues of various clinical stages
Improved prescription of taohechengqi-tang alleviates D-galactosamine acute liver failure in rats
Development of a Xeno-Free Feeder-Layer System from Human Umbilical Cord Mesenchymal Stem Cells for Prolonged Expansion of Human Induced Pluripotent Stem Cells in Culture
Impacts of Rho kinase inhibitor Fasudil on Rho/ROCK signaling pathway in rabbits with optic nerve injury
Chronic sciatic nerve compression induces fibrosis in dorsal root ganglia
The COX-2-Selective Antagonist (NS-398) Inhibits Choroidal Neovascularization and Subretinal Fibrosis
Cellular response of chondrocytes to magnesium alloys for orthopedic applications
Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells
Tissue-Engineered Regeneration of Completely Transected Spinal Cord Using Induced Neural Stem Cells and Gelatin-Electrospun Poly (Lactide-Co-Glycolide)/Polyethylene Glycol Scaffolds
Protective Effects of Astragaloside IV on db/db Mice with Diabetic Retinopathy
Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits
Sequential release of salidroside and paeonol from a nanosphere-hydrogel system inhibits ultraviolet B-induced melanogenesis in guinea pig skin
Effects of minimally invasive procedures for evacuation of intracerebral hematoma in early stages on MMP-9 and BBB permeability in rabbits
Characterization of the Mechanism of Inhibin ?-Subunit Gene in Mouse Anterior Pituitary Cells by RNA Interference
Perihematomal glutamate level is associated with the blood?brain barrier disruption in a rabbit model of intracerebral hemorrhage
Effect of advanced glycation end products on the expression of hypoxia-inducible factor-1? and vascular endothelial growth factor proteins in RF/6A cells
Gene Expression Analyses of Subchondral Bone in Early Experimental Osteoarthritis by Microarray
Lin L, Jia Z, Zhao Y, Wu Y, Zhao X, Li Y, Guo Z, Chen J, Cheng S, Wang D, Ruan D. Biomed Res Int. 2017;2017:9765843. doi: 10.1155/2017/9765843. Epub 2017 Oct 8. Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity
Yu X, Lin H, Wang Y, Lv W, Zhang S, Qian Y, Deng X, Feng N, Yu H, Qian B. Onco Targets Ther. 2018 Apr 4;11:1833-1847. doi: 10.2147/OTT.S155716. eCollection 2018. d-limonene exhibits antitumor activity by inducing autophagy and apoptosis in lung cancer
Mo BY, Guo XH, Yang MR, Liu F, Bi X, Liu Y, Fang LK, Luo XQ, Wang J, Bellanti JA, Pan YF, Zheng SG. Front Immunol. 2018 Apr 10;9:702. doi: 10.3389/fimmu.2018.00702. eCollection 2018. Long Non-Coding RNA GAPLINC Promotes Tumor-Like Biologic Behaviors of Fibroblast-Like Synoviocytes as MicroRNA Sponging in Rheumatoid Arthritis Patients
Liu Q, Sun B, Zhao J, Wang Q, An F, Hu X, Yang Z, Xu J, Tan M, Li L. Exp Mol Med. 2018 May 7;50(5):60. doi: 10.1038/s12276-018-0088-z. Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis
Chen WZ, Li YM, Yu X, Li Y, Li WK, Wang QL, Liang AX, Li X, Yang LG, Han L. Microb Biotechnol. 2018 Jan;11(1):248-256. doi: 10.1111/1751-7915.13029. Epub 2017 Dec 4. The efficacy, biodistribution and safety of an inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis
Li K, Gesang L, He C. Exp Ther Med. 2017 Oct;14(4):3780-3787. doi: 10.3892/etm.2017.4996. Epub 2017 Aug 22. Mechanism of apoptosis involved in gastric mucosal lesions in Tibetans with high-altitude polycythemia
Li J, Deng J, Yuan J, Fu J, Li X, Tong A, Wang Y, Chen Y, Guo G. Int J Nanomedicine. 2017 Mar 29;12:2443-2456. doi: 10.2147/IJN.S128705. eCollection 2017. Zonisamide-loaded triblock copolymer nanomicelles as a novel drug delivery system for the treatment of acute spinal cord injury
Fang X, Li Y, Qiao J, Guo Y, Miao M. Mol Med Rep. 2017 Nov;16(5):7439-7449. doi: 10.3892/mmr.2017.7540. Epub 2017 Sep 20. Neuroprotective effect of total flavonoids from Ilex pubescens against focal cerebral ischemia/reperfusion injury in rats
Jiang Y, Zhao Y, Chen S, Chen L, Li C, Zhou X. Reprod Biol Endocrinol. 2018 Mar 20;16(1):25. doi: 10.1186/s12958-018-0348-8. Regulation by FSH of the dynamic expression of retinol-binding protein 4 in the mouse ovary
Liu Q, Wu C, Huang S, Wu Q, Zhou T, Liu X, Liu X, Hu X, Li L. Int J Mol Med. 2018 May;41(5):2609-2618. doi: 10.3892/ijmm.2018.3489. Epub 2018 Feb 13. Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury
Tian X, Liu C, Wang X, Wang F, Wang L, Xu L, Ma J, Gao Y, Bao Y, Wang F, Sun L, Wei J, Lin C, Zhang H, Zhu G, Guan X, Fu S, Zhang C. Cell Death Dis. 2018 Mar 1;9(3):341. doi: 10.1038/s41419-018-0362-y. Sei-1 promotes double minute chromosomes formation through activation of the PI3K/Akt/BRCA1-Abraxas pathway and induces double-strand breaks in NIH-3T3 fibroblasts
Cai XJ, Wang L, Hu CM. Biosci Rep. 2018 Jan 19;38(1). pii: BSR20171184. doi: 10.1042/BSR20171184. Print 2018 Feb 28. Effects of GABAB receptor activation on spatial cognitive function and hippocampal neurones in rat models of type 2 diabetes mellitus.
Ou, Z., Kong, X., Sun, X., He, X., Zhang, L., Gong, Z.,..., & Xuan, A. Metformin treatment prevents amyloid plaque deposition and memory impairment in APP/PS1 mice. Brain, Behavior, and Immunity. Advance online publication. doi: 10.1016/j.bbi.2017.12.009
Cheng, B., Zheng, H., Wu, F., Wu, J., Liu, X., Tang, C.,..., & Su, Z. (2017). Metabolomics analysis of Danggui Sini decoction on treatment of collagen-induced arthritis in rats. Journal of Chromatography B, 1061-1062, 282-291. doi: 10.1016/j.jchromb.2017.07.043
Mei, F., Wang, J.G., Chen, Z.J., & Yuan, Z.L. (2017). Effects of epoxyeicosatrienoic acids (EETs) on retinal macular degeneration in rat models. European Review for Medical and Pharmacological Sciences, 21(12), 2970-2979. Retrieved from http://www.europeanreview.org/article/12960
Liu, X., Li, Y., He, J., Zhao, T., Chen, C., Gu, H., & Wang, X. (2017). Paclitaxel-loaded pluronic F127/P123 silica nanocapsules with surface conjugated rhTRAIL for targeted cancer therapy. RSC Advances, 7(48), 30250–30261. Advance online publication. doi: 10.1039/c7ra04503d
Ren, J., Lin, L., Wang, X., Cai, Z., Li, C., Li, D., & Chang, Q. (2017). Role of Chloride Channel-3 (ClC-3) in SIN-1-induced apoptosis in rat hippocampal neurons. Biomedical Research, 28(9), 3991-3995. Retrieved from http://www.alliedacademies.org/articles/role-of-chloride-channel3-clc3-in-sin1induced-apoptosis-in-rat-hippocampal-neurons.pdf
Liu Q, Long Z, Dong X, Zhang T, Zhao J, Sun B, Zhu J, Li J, Wang Q, Yang Z, Hu X, Li L. Exp Mol Med. 2017 Apr 21;49(4):e319. doi: 10.1038/emm.2017.31. Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.
Wang J, Yang Z, Wen J, Ma F, Wang F, Yu K, Tang M, Wu W, Dong Y, Cheng X, Nie C, Chen L. J Pharmacol Sci. 2014;126(3):198-207. Epub 2014 Oct 21. Sklb-M8 Induces Apoptosis Through The Akt/Mtor Signaling Pathway In Melanoma Models And Inhibits Angio...
Wang Q, Zhu J, Zou L, Yang Y. J Huazhong Univ Sci Technolog Med Sci. 2011 Apr;31(2):246-50. Doi: 10.1007/S11596-011-0261-2. Epub 2011 Apr 20. Role Of Axonal Guidance Factor Netrin-1 In Human Placental Vascular Growth.
Zhao Jw, Gao Zl, Mei H, Li Yl, Wang Y. Am J Med Sci. 2011 Jun;341(6):460-8. Doi: 10.1097/Maj.0B013E31820865D5. Differentiation Of Human Mesenchymal Stem Cells: The Potential Mechanism For Estrogen-Induced Preferential Osteoblast Versus Adipocyte D...
Zhang P, Wu X, Hu C, Wang P, Li X. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):30-6. Doi: 10.1007/S11626-011-9471-Y. Epub 2011 Dec 17. Rho Kinase Inhibitor Y-27632 And Accutase Dramatically Increase Mouse Embryonic Stem Cell Derivation.
Sun Y, Chen S, Song G, Ren L, Wei L, Liu N, Zhang D, Lv X. Int J Mol Med. 2012 Sep;30(3):622-8. Doi: 10.3892/Ijmm.2012.1032. Epub 2012 Jun 14. Effect Of Visfatin On The Function Of Endothelial Progenitor Cells In High-Fat-Fed Obese Rat...
Wang Y, Li Jr, Sun Mx, Ni B, Huan C, Huang L, Li C, Fan Hj, Ren Xf, Mao X. Antiviral Res. 2014 Jun;106:33-41. Doi: 10.1016/J.Antiviral.2014.03.007. Epub 2014 Mar 25. Triggering Unfolded Protein Response By 2-Deoxy-D-Glucose Inhibits Porcine Epidem...
Liu C, Xia X, Miao W, Luan X, Sun L, Jin Y, Liu L. Int J Pharm. 2013 Oct 15;455(1-2):312-9. Doi: 10.1016/J.Ijpharm.2013.07.004. Epub 2013 Jul 15. An Ovarian Cell Microcapsule System Simulating Follicle Structure For Providing Endogenous Female Hor...
Jin F, Li L, Shi M, Li Z, Zhou J, Chen L. Behav Brain Res. 2013 Jun 1;246:116-24. Doi: 10.1016/J.Bbr.2013.02.029. Epub 2013 Mar 15. The Longitudinal Study Of Rat Hippocampus Influenced By Stress: Early Adverse Experience Enhances Hippocampal Vulne...
Chen F, Wang H, Xiang X, Yuan J, Chu W, Xue X, Zhu H, Ge H, Zou M, Feng H, Lin J. J Surg Res. 2014 Dec;192(2):298-304. Doi: 10.1016/J.Jss.2014.06.026. Epub 2014 Jun 19. Curcumin Increased The Differentiation Rate Of Neurons In Neural Stem Cells Vi...
Wang J, Ren Y, Guo X, Cheng H, Ye Y, Qi J, Yang C, You H. Mol Med Rep. 2015 May;11(5):3585-92. Doi: 10.3892/Mmr.2015.3164. Epub 2015 Jan 8. Alterations In Enhancer Of Zeste Homolog 2, Matrix Metalloproteinase-2 And Tissue Inhibitor Of Metalloprote...
Zhang R, Fang H, Chen Y, Shen J, Lu H, Zeng C, Ren J, Zeng H, Li Z, Chen S, Cai D, Zhao Q. Plos One. 2012;7(2):E32356. Doi: 10.1371/Journal.Pone.0032356. Epub 2012 Feb 27. Gene Expression Analyses Of Subchondral Bone In Early Experimental Osteoart...
Pang W, Zhang Y, Zhao N, Darwiche Ss, Fu X, Xiang W. Placenta. 2013 Jul;34(7):613-8. Doi: 10.1016/J.Placenta.2013.03.013. Epub 2013 Apr 17. Low Expression Of Mfn2 Is Associated With Mitochondrial Damage And Apoptosis In The Placental Villi Of Earl...
Peterson Am, Pilz-Allen C, Kolesnikova T, M??hwald H, Shchukin D. Acs Appl Mater Interfaces. 2014 Feb 12;6(3):1866-71. Doi: 10.1021/Am404849Y. Epub 2013 Dec 16. Growth Factor Release From Polyelectrolyte-Coated Titanium For Implant Applications.
Xing J, Jin H, Hou T, Chang Z, Luo F, Wang P, Li Z, Xie Z, Xu J. J Surg Res. 2014 Dec;192(2):454-63. Doi: 10.1016/J.Jss.2014.05.037. Epub 2014 May 27. Establishment Of A Bilateral Femoral Large Segmental Bone Defect Mouse Model Potentially Applica...
Ding Y, Yuan S, Liu X, Mao P, Zhao C, Huang Q, Zhang R, Fang Y, Song Q, Yuan D, Xie P, Liu Y, Liu Q. Plos One. 2014 Nov 20;9(11):E112207. Doi: 10.1371/Journal.Pone.0112207. Ecollection 2014. Protective Effects Of Astragaloside Iv On Db/Db Mice Wit...
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Customer Q&As

Q: is AR1068 0.2 um filtered?
A; AR1068 isn not 0.2 um filtered.
Q: How long can this product be stored after being opened at -20°C?
A: Please use this product within the shelf life of one year at -20°C after the solution is opened
Q: Can I dilute AR1068 from 4% to 1% Paraformaldehyde (PFA) solution in PBS? What is the protocol? Keywords: dilution, concentration
A: Yes, the 4% Paraformaldehyde can be diluted to 1% in PBS. The dilution protocol is as follows: (1) Prepare the PBS by adding 14.2g Na2HPO4 and 9g NaCl into 1000ml H2O. (2) Mix 500mL 4% Paraformaldehyde with 1500mL PBS thoroughly.
Q: How pure (%) is the PFA in your pre-made solution?
A: Purity of the solid formaldehyde is ≥95%.
Q: We will use this to fix bacterial cells from faecal samples. Do you have any reference for this use?
A: We haven't use AR1068 to fix bacterial cells from faecal samples. We could not provide any reference for this use. We're sorry for the inconvenience.
Q: Since we will be using this on a daily basis, should it be stored at room temperature or at 4°C? What is the shelf life for these conditions?
A: Please store the product at 4°C if it is frequently used. The shelf life is 3 months at 4°C. For long storage, please store at -20°C.
Q: Is this product DNA/RNA free?
A: Yes, AR1068 is DNA/RNA free.
Q: What is the PBS concentration?
A: PBS concentration is 0.01g/mL.
Q: What is the detailed composition of AR1068?
A: AR1068 contains paraformaldehyde, Na2HPO4, NaCl, and DEPC.
Q: Is the 4% PFA solution in PBS methanol free?
A: Yes, it is methanol free.