4% Paraformaldehyde (PFA) Solution in PBS

4% Paraformaldehyde solution in PBS is a ready-to-use solution for sample preparation and fixing cells for immunohistochemistry (IHC). Cited in 10 publication(s).

Product Info Summary

SKU: AR1068
Size: 500mL

General Description

Paraformaldehyde (PFA) is the polymerization product of formaldehyde with a typical degree of polymerization of 8–100 units. Paraformaldehyde is not a fixative itself; it must be depolymerized to formaldehyde in solution. In cell culture, typical formaldehyde fixing procedure would involve using a 4% formaldehyde solution in phosphate buffered saline (PBS) on ice for 10 minutes. Fixing ensures that sample cell structures stay intact and that antigens are immobilized, while ideally still permitting unfettered access of antibodies to target antigens.

Our 4% Paraformaldehyde is the most preferred fixative agent as it builds covalent cross-links between molecules. This glues them together hence effectively preserving cells and tissue components. Use of this 4% paraformaldehyde can guarantee consistency in the physical and chemical properties of the cell, hence no change in chemical and morphology characteristics of the cells and tissues.

Since the 4% paraformaldehyde is not fixative itself, it is required that formaldehyde is freshly prepared from the PFA stock.

Overview

Product Name 4% Paraformaldehyde (PFA) Solution in PBS
SKU/Catalog Number AR1068
FormLiquid
Physical State The solution should be clear, colorless, with no precipitate.
Pack Size 500mL
Application Preserving Samples, Fixing Cells For Histology
*Our Boster Guarantee covers the use of this product in the above tested applications.
StorageStore at -20˚C for one year.
Cite This Product 4% Paraformaldehyde (PFA) Solution in PBS (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1068)
Equivalent N/A

Properties

CAS Number30525-89-4
MDL NumberMFCD00133991
Solubility Soluble in water
pH 7.0 - 7.6 at 25°C

Safety and Handling

•Causes serious eye damage .

• Suspected of causing cancer.

• May cause an allergic skin reaction.

• Causes skin irritation.

• Avoid breathing dust/fume/gas/mist/vapors/spray.

• Wear protective gloves/protective clothing/eye protection/face protection.

•IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

• Store locked up.

•Dispose of contents/container in accordance with local/regional/national/international regulations.

General Notes

• Adjust pH to 7.2-7.6 with NaH2PO4 if pH <7 after long storage.

• Deposition is normal for this product.

4% Paraformaldehyde Recipe

To prepare a solution of 4% paraformaldehyde you need to use this protocol. The following ingredients are required:

•1M Sodium Hydroxide

•10X phosphate-buffered saline (PBS)

•1M Hydrochloric acid

Note: This should be done in a fume chamber with gloves, safety glasses, and a face mask.

1. To prepare a 4% of paraformaldehyde (PFA) solution in PBS, add 4 grams of granular or prill paraformaldehyde to water (H2O)

2. Use stirring hot plate to heat the solution to 60-degree celsius. Add 1mL of 1M NaOH until the paraformaldehyde is fully dissolved.

3. Add 10 mL of 10x phosphate-buffered saline (PBS) and allow the mixture to cool.

4. Adjust the solution pH with 1M HCL then top up the volume to 100 mL with H2O.

5. Filter the solution to remove any deposit.

Validation Images & Assay Conditions

Hello CJ!

AR1068 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

miR-335 targets CRIM1 to promote the proliferation and inhibit the apoptosis of placental trophoblast cells in preeclamptic rats

LIPUS promotes synthesis and secretion of extracellular matrix and reduces cell apoptosis in human osteoarthritis through upregulation of SOX9 expression

NUDCD1 promotes metastasis through inducing EMT and inhibiting apoptosis in colorectal cancer

Effects of epoxyeicosatrienoic acids (EETs) on retinal macular degeneration in rat models.

High expression of SLC26A6 in the kidney may contribute to renal calcification via an SLC26A6-dependent mechanism

LY294002 Induces G0/G1 Cell Cycle Arrest and Apoptosis of Cancer Stem-like Cells from Human Osteosarcoma Via Down-regulation of PI3K Activity

Propranolol induced apoptosis and autophagy via the ROS/JNK signaling pathway in Human Ovarian Cancer

Jiedu Sangen Decoction Reverses Epithelial-to-mesenchymal Transition and Inhibits Invasion and Metastasis of Colon Cancer via AKT/GSK-3β Signaling Pathway

Metformin Inhibits Tumorigenesis and Tumor Growth of Breast Cancer Cells by Upregulating miR-200c but Downregulating AKT2 Expression

Minimally Invasive Surgery for Intracerebral Hematoma Evacuation followed by Rosiglitazone Infusion Therapy Increased Perihematomal Occludin, Zonula Occludens-1 Expression and Decreased the Blood Brain Barrier Permeability in Rabbits

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15 Customer Q&As for 4% Paraformaldehyde (PFA) Solution in PBS

Question

Does AR1068 contain any methanol?

Verified customer

Asked: 2022-04-19

Answer

The 4% Paraformaldehyde (PFA) Solution In PBS (AR1068) is methanol free.

Boster Scientific Support

Answered: 2022-04-19

Question

Is this PFA solution is ready to be used for fixing. Or does it require any more preparations to be used for immunostaining?

Verified customer

Asked: 2022-02-28

Answer

This PFA solution is ready to be used for fixing.

Boster Scientific Support

Answered: 2022-02-28

Question

Can AR1068 be used for electron microscopy biological sample preparation?

Verified customer

Asked: 2020-10-19

Answer

The 4% Paraformaldehyde (PFA) Solution In PBS (AR1068) can not be used directly for electron microscopy. It could mix with other reagents such as glutaraldehyde to be used for electron microscopy.

Boster Scientific Support

Answered: 2020-10-20

Question

Do you think AR1068 is suitable to fix cells prior to analysis by Flow Cytometry?

Verified customer

Asked: 2020-06-29

Answer

The 4% Paraformaldehyde (PFA) Solution In PBS (AR1068) is suitable to fix cells prior to analysis by Flow Cytometry.

Boster Scientific Support

Answered: 2020-07-01

Question

How long can this product be stored after being opened at -20°C?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

Please use this product within the shelf life of one year at -20°C after the solution is opened

Boster Scientific Support

Answered: 2020-04-29

Question

is AR1068 0.2 um filtered?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

A

Boster Scientific Support

Answered: 2020-01-16

Question

Is the 4% PFA solution in PBS methanol free?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

Yes, it is methanol free.

Boster Scientific Support

Answered: 2019-11-14

Question

Can I dilute AR1068 from 4% to 1% Paraformaldehyde (PFA) solution in PBS? What is the protocol? Keywords: dilution, concentration

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

Yes, the 4% Paraformaldehyde can be diluted to 1% in PBS. The dilution protocol is as follows: (1) Prepare the PBS by adding 14.2g Na2HPO4 and 9g NaCl into 1000ml H2O. (2) Mix 500mL 4% Paraformaldehyde with 1500mL PBS thoroughly.

Boster Scientific Support

Answered: 2019-11-08

Question

How pure (%) is the PFA in your pre-made solution?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

Purity of the solid formaldehyde is ≥95%.

Boster Scientific Support

Answered: 2019-09-24

Question

Is AR1068 PFA solution DepC RNAse free?

Verified customer

Asked: 2019-07-12

Answer

Our 4% Paraformaldehyde (PFA) Solution In PBS (AR1068) contains DepC RNAse.

Boster Scientific Support

Answered: 2019-07-12

Question

Since we will be using this on a daily basis, should it be stored at room temperature or at 4°C? What is the shelf life for these conditions?

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

Please store the product at 4°C if it is frequently used. The shelf life is 3 months at 4°C. For long storage, please store at -20°C.

Boster Scientific Support

Answered: 2018-12-14

Question

What is the PBS concentration?

Verified Customer

Verified customer

Asked: 2017-10-06

Answer

PBS concentration is 0.01g/mL.

Boster Scientific Support

Answered: 2017-10-06

Question

What is the detailed composition of AR1068?

Verified Customer

Verified customer

Asked: 2017-05-08

Answer

AR1068 contains paraformaldehyde, Na2HPO4, NaCl, and DEPC.

Boster Scientific Support

Answered: 2017-05-08

Question

Is this product DNA/RNA free?

Verified Customer

Verified customer

Asked: 2015-11-09

Answer

Yes, AR1068 is DNA/RNA free.

Boster Scientific Support

Answered: 2015-11-09

Question

We will use this to fix bacterial cells from faecal samples. Do you have any reference for this use?

Verified Customer

Verified customer

Asked: 2014-12-26

Answer

We haven't use AR1068 to fix bacterial cells from faecal samples. We could not provide any reference for this use. We're sorry for the inconvenience.

Boster Scientific Support

Answered: 2014-12-26

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