Because we are an antibody manufacturer with ELISA expertise
Since 1993, Boster has been manufacturing antibodies and ELISA kits for scientists around the world. Our founder developed Picokine®, a proprietary high sensitivity sandwich ELISA platform, in the early 2000s. Over past two decades we have developed 1000+ high sensitive Picokine® ELISA kits which have been cited in over 6000 publications to end of 2019 (source: Google scholar, Bioz and CiteAb). Hundreds of ELISA assays are run each week by the ELISA experts at Boster Bio. With such history and scale, Boster is able to provide you a unique combination of service quality, subject expertise and cost savings, when it comes to ELISA service.
On this page
Service Details: workflow, phases
Are you looking for custom ELISA development Service?
This page is for ELISA service, where we perform the experiment as a CRO service for you. If you want to develop custom ELISA please check out our custom monoclonal antibody service page. If you would like to validate existing antibody pairs and development them into ELISA (both multiplex and singleplex format available) please inquire at [email protected] for ELISA development service.
In a nutshell: you send us your samples, we test them for your analytes of interest and send you the results data.
Typical ELISA service workflow
Experienced ELISA development, tailored to achieve your goals.
Enzyme-linked immunosorbent assay (ELISA) is one of the most common methods for quantification of analytes in samples. Projects we run for our clients ranges from simple outsourced ELISAs to assay development for various applications (clinical, allergen etc.). Typically we go through the following workflow to engage each client to provide the best tailored service.
Assay Design Consultation
We discuss the goals of your assays and help you decide on analytes/biomarkers, format and configuration. We have worked with experts in many subject matters and can provide scientific consultation for our customers, in addition to simple outsourcing capacity.
We pick the right assay for you. Boster carries 1000+ high sensitivity sandwich ELISA kits, and a wide range of other format ELISA kits. If you want to work with ELISA kits from other companies, we can procure them for you, or you can send your own ELISA kits to us. If no kit is available, we can develop assay from antibody pairs. Certain antibody combinations are best for specific assay types. For example, in sandwich ELISAs, one antibody traps the analyte, and the other antibody is used to detect. These pairs are tested for compatibility.
Turn around time: typically 5-20 days
We test standard samples, matrices, conjugates, etc. in combination to create the prototype. We help you select standards and set aside key controls, etc.
Technical Transfer Or Scaling Up
We ramp up the testing scale for you, or we transfer the technology to your laboratory, ensuring that the assay works in your technicians' hands. We can also custom manufacture these kits for you if you want to develop them into a product.
Note: Due to the variety of methods and materials used in preparation, tissue homogenates and cell lysates have not been validated for this service. If you choose to send these samples types, please be aware we are unable to guarantee satisfactory results.
Sample Testing Requirements
For general reference only. Detailed instructions will be given for each project
Sample Types - The samples can be serum, plasma, cell
culture supernatant, cell lysate, cell culture media,
and/or tissue homogenates. Make sure to specify the type
of sample and buffer constituents used for the samples when you contact us. Clearly label each vial using permanent marking
and provide a list of the samples in the shipment. The
labeling should exactly match the digital manifest.
Note: Due to the variety of methods and
materials used in preparation, tissue homogenates and cell
lysates have not been validated for this service. If you
choose to send these samples types, please be aware we are
unable to guarantee satisfactory results.
Sample Volume - It is optimal for at least 200
μl of each sample to be sent per analyte. Based on
your request, an additional volume may be needed. This
volume allows for triplicate testing of
Minimum Number of Samples - 8 samples
minimum. Please contact Boster for
price quotes if requesting sample testing for less than
the minimums listed.
Sample should be shipped overnight, Monday through
Thursday, frozen with 3-5 lbs dry ice-
Protect the samples from damage by putting them in a box or
wrapping with bubble wrap, so the samples are not loose among the dry ice as
this will damage the vials and labeling.
For research use only. Not intended for diagnostic
Turnaround Time - A completed report of the samples will
be emailed to the customer. Typical turnaround times range
from 5 to 20 business days based on the assays requested. The
quote provided to the customer will give a firm estimate of
the time to receive data.
Reporting Results - All results will be reported in a
Microsoft Excel spreadsheet format. The spreadsheet will
contain raw data, charts, and calculated concentrations
derived from regression models from each of the samples
tested. A written report explaining the technology is also
Samples will be destroyed 3 months after the date of
receipt. If requested at the time the order is placed,
samples will be returned to the customer at the customer's
Ready to start?
To receive a consultation and quote from one of our scientists, submit your project details to [email protected] or through the live chat. We will respond within two business days. If you require immediate assistance, call us at (888) 466-3604.