Buy Primary Get Secondary FREE
For a limited time, Boster will match your primary antibodies with a FREE HRP conjugated secondary antibody for WB and IHC. This promotion is applicable to all countries. The secondary antibody has to be ordered with the primary antibody to qualify. Browse qualified secondary antibodies now and take advantage of this promotion.
For every two primary antibodies ordered you can trade the secondary antibodies for a Super Vision kit-Boster's proprietary All-In-One two-step polymer-based IHC kit, that saves you the step of having to add SABC to amplify the signal.
For every two primary antibodies ordered you can trade the secondary antibodies for an All-In-One style Enhanced Chemiluminescent Kit (against Mouse IgG or Rabbit IgG) for Western Blotting.
*Free secondary antibodies must be ordered together with primary antibodies to qualify. Contact us for any questions.
Secondary Antibody Overview
Boster’s secondary antibodies are affinity-purified antibodies with well-characterized specificity for the primary antibodies' Fc regions. These secondary antibodies are used to detect their specified targets. Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies’ FC regions provide further binding locations for biotins, enabling the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.
Boster’s conjugated secondary antibodies are designed for applications in ELISA, colorimetric western blot (WB), chemileuminescent western blot (WB), dot blot, immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry (FC), immunofluorescence (IF) and electron microscopy (EM). We offer secondary antibodies conjugated to a variety of conjugates including Biotin, HRP, metal (colloidal gold) and fluorescent dyes (FITC, TRITC and Cy3).
The following table helps you quickly find the secondary antibody you need.
| Target || Host: Rabbit || Host: Goat || Host: Mouse |
| Avidin || HRP || || |
| goat IgG || Unconjugated, TRITC, HRP, FITC, Cy3, 10 nm Colloidal Gold, 5 nm Colloidal Gold, Biotin || || |
| human IgA || || FITC, HRP || |
| human IgG || Unconjugated, TRITC, HRP, FITC, Cy3, Biotin || || FITC, Biotin |
| human IgM || || Biotin, FITC, HRP || |
| mouse IgG || Unconjugated || DyLight®488, Biotin, 5 nm Colloidal Gold, 10 nm Colloidal Gold, Cy3, FITC, HRP, TRITC, Unconjugated || |
| mouse IgM || || Biotin, HRP || |
| rabbit IgG || || DyLight®488, Biotin, 5 nm Colloidal Gold, 10 nm Colloidal Gold, Cy3, FITC, HRP, TRITC, Unconjugated || Biotin |
| rat IgG || Unconjugated, HRP, FITC, Biotin || || |