Boster provides all the reagents you will need for performing your Western blotting SOP and more. See below for a detailed listing of our Western blot reagents and analysis kits sorted by the function/step they are used for in a typical western blot experiment protocol.

We provide a PDF for Western blotting protocols and troubleshooting tips. You can download it here

Solutions for western blotting workflow

All-In-One WB Kits Sample Preparation Total Protein Analysis
Electrophoresis/Gel Staining Protein Transfer Blocking Reagents
Chromogenic Development Chemiluminescent Development Ready-to-use Lysates

Check out the Boster technical newsletter archive which includes short blog articles for various experimental applications such as Western Blot, Immunohistochemistry (IHC), ELISA, and Flow Cytometry. These technical articles offer troubleshooting advice, protocol optimization tips, research pathway maps, and more! You can view archive here

3 Guidelines for Using Secondary Antibodies

Apr 16, 2017

Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies

The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.

SKU AR1196
Pack size 50mL
Applications To detect antigen at low-picogram to mid-femtogram level by reacting with horseradish peroxidase

Availability: Ships in 5-7 business days

Pack Size:1 pack (It is sufficient to make 500 ml of TBS wash buffer)
Application:ELISA, IHC, WB
Availability: Ships next business day
Pack Size:1 pack (It is sufficient to make 200 ml of TBS blocking buffer)
Application:WB
Availability: Ships next business day
Pack Size:12ml

Availability: Ships in 5-7 business days

SKU AR0173
Pack size 9 cm×7.5 cm
Applications Protein transfer from SDS-PAGE gels to membrance in preparation for western blotting
Availability: Ships next business day
SKU AR0172
Pack size 12.5 cm×12.5 cm
Applications Protein transfer from SDS-PAGE gels to membrance in preparation for western blotting

Availability: Ships in 5-7 business days

SKU AR1149
Pack size 1L/pack
Applications Transfer buffer for Tris-glycine-sds gel
Availability: Ships next business day
Pack Size:0.45um, 9cm × 10 cm; 20 sheets
Availability: Ships next business day
Pack Size:0.22um, 9cm × 10 cm; 20 sheets
Availability: Ships next business day
Pack Size:1kit
Availability: Ships next business day
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