Boster provides all the reagents you will need for performing your Western blotting SOP and more. See below for a detailed listing of our Western blot reagents and analysis kits sorted by the function/step they are used for in a typical western blot experiment protocol.

We provide a PDF for Western blotting protocols and troubleshooting tips. You can download it here

Solutions for western blotting workflow

All-In-One WB Kits Sample Preparation Total Protein Analysis
Electrophoresis/Gel Staining Protein Transfer Blocking Reagents
Chromogenic Development Chemiluminescent Development Ready-to-use Lysates

Check out the Boster technical newsletter archive which includes short blog articles for various experimental applications such as Western Blot, Immunohistochemistry (IHC), ELISA, and Flow Cytometry. These technical articles offer troubleshooting advice, protocol optimization tips, research pathway maps, and more! You can view archive here

3 Guidelines for Using Secondary Antibodies

Apr 16, 2017

Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies

The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.

SKU AR1163
Pack size 100 mL
Form Liquid
Applications Stacking gel buffer for casting polyacrylamide gels
*Our Boster Guarantee covers the use of this product in the above tested applications.

Availability: Ships in 5-7 business days

SKU AR1182
Pack size 1mLX2
Form Solution (liquid)
Applications Western blotting, protein purification, Co-IP; assays for protein expression, activity, modification, profiling and characterization, quantitative measurement; epitope tagging; reporter gene analysis
Availability: Ships next business day
SKU AR0153
Pack size 100 mL (for 10 assays for an 5 × 8.5cm blot)
Applications Reuse a nitrocellulose or PVDF blot to detect a different target with a different primary antibody
Reprobe a blot to correct or optimize antibody concentrations that were ineffective the first time
Availability: Ships next business day
SKU AR0132
Pack size 1kit (Enough for 10 assays)
FormLiquid
Description Boster’s WB Developing Fixing Kit is a processing kit, which can be used in Western, Northern and Southern Blotting after squashed. This kit is especially suitable for X-ray developing and fixing, as well as normal black & white negative film or photograph.
Availability: Ships next business day
SKU AR1155
Pack size 500mL/pack
Applications WB, ELISA and IHC
Availability: Ships next business day
SKU AR0144
Pack size 500mL/pack
Applications WB, ELISA and IHC
Availability: Ships next business day
SKU AR0143
Pack size 200mL/pack
Applications WB only
Availability: Ships next business day
SKU AR0142
Pack size 12 mL(10X)
FormLiquid
Applications onceau S solution has been used for membrane staining (Western blotting)

Availability: Ships in 5-7 business days

SKU AR0173
Pack size 100 sheets
Applications Protein transfer from SDS-PAGE gels to membrance in preparation for western blotting
Availability: Ships next business day
SKU AR0172
Pack size 12.5 cm×12.5 cm
Applications Protein transfer from SDS-PAGE gels to membrance in preparation for western blotting
Availability: Ships next business day
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