Rabbit Monoclonal Antibodies

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  1. Anti-Apolipoprotein A I Rabbit Monoclonal Antibody M00717
    $299.00
    • Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
    • 100μg/vial
    • Human, Mouse
    • IP, IF, IHC, ICC, WB
    Anti-Apolipoprotein A I Rabbit Monoclonal Antibody tested for IP, ICC/IF, IHC, WB in Human, Mouse Learn More
  2. Anti-Histone H3 (mono+di+tri methyl K14) Rabbit Monoclonal Antibody M12477-9
    $299.00
    • Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
    • 100ug/vial
    • Human, Mouse
    • IF, WB
    Anti-Histone H3 (mono+di+tri methyl K14) Rabbit Monoclonal Antibodytested for IF, ICC, WB in Human, Mouse Learn More
  3. Anti-Histone H3 (mono+di+tri methyl K79) Rabbit Monoclonal Antibody M12477-8
    $299.00
    • Polyclonal Antibodies, IHC ICC IF Antibodies
    • 100ug/vial
    • Human, Mouse, Rat
    • IHC, WB
    Anti-Histone H3 (mono+di+tri methyl K79) Rabbit Monoclonal Antibody tested for IHC, WB in Human, Mouse, Rat Learn More
  4. Anti-Androgen Receptor (AR-V7 specific) Rabbit Monoclonal Antibody M00542-3
    $299.00
    • Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
    • 100ug/vial
    • Human
    • IHC, WB
    Anti-Androgen Receptor (AR-V7 specific) Rabbit Monoclonal Antibody tested for IHC, WB in Human Learn More
  5. Anti-K63-linkage Specific Ubiquitin Rabbit Monoclonal Antibody M02848-2
    $299.00
    • Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
    • 100ug/vial
    • Human, Mouse, Rat
    • IF, WB
    Anti-K63-linkage Specific Ubiquitin Rabbit Monoclonal Antibodytested for Flow Cytometry, IF, IHC, ICC, WB in Human, Mouse, Rat Learn More
  6. Anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody M02848-1
    $299.00
    • Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
    • 100ug/vial
    • Human, Mouse, Rat
    • Flow Cytometry, IF, IHC, ICC, WB
    Anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody tested for Flow Cytometry, IF, ICC, IHC, WB in Human, Mouse, Rat Learn More
  7. Anti-Stromal interaction molecule 1 Rabbit Monoclonal Antibody M00312
    $299.00
    • Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
    • 100ug/vial
    • Human, Mouse, Rat
    • IP, WB
    Anti-Stromal interaction molecule 1 Rabbit Monoclonal Antibodytested for IP, IHC, WB in Human, Mouse, Rat Learn More
  8. Anti-Histone H2B (mono methyl R79) Rabbit Monoclonal Antibody M07286
    $299.00
    • Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies
    • 100ug/vial
    • Human, Mouse
    • WB
    Anti-Histone H2B (mono methyl R79) Rabbit Monoclonal Antibody tested for WB in Human, Mouse Learn More
  9. Anti-Histone H3 (mono methyl R128) Rabbit Monoclonal Antibody M12477-7
    $299.00
    • Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies
    • 100ug/vial
    • Human, Mouse
    • WB
    Anti-Histone H3 (mono methyl R128) Rabbit Monoclonal Antibody tested for WB in Human, Mouse Learn More
  10. Anti-Retinoic Acid Receptor alpha Rabbit Monoclonal Antibody M00392
    $299.00
    • Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies
    • 100ug/vial
    • Human, Mouse
    • WB
    Anti-Retinoic Acid Receptor alpha Rabbit Monoclonal Antibodytested for Flow Cytometry, WB in Human, Mouse Learn More

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What are rabbit monoclonal antibodies?

Monoclonal antibodies are used as primary antibodies in research because of their high specificity for a unique epitope of an antigen, which also leads to minimal lot-to-lot variation and low non-specific cross-reactivity. After the discovery of the hybridoma technique designed by Caesar Millstein and Georges Kohler to produce monoclonal antibodies in 1975, monoclonal antibodies have been conventionally developed in mice for various applications. Over the years, other new techniques have led to the emergence of monoclonal antibodies raised against rabbits, rats, and hamsters. Although mouse monoclonal antibodies have remained as the most commonly used type of monoclonal antibody, a 2015 CiteAb study observed accelerating growth in the number of researchers using rabbit monoclonal antibodies while mouse monoclonals have experienced a steady decrease in the percentage of citations.

Many researchers have been choosing rabbit monoclonal antibodies over mouse monoclonal antibodies for their high specificity, affinity, and sensitivity. BosterBio offers over 500 high quality rabbit monoclonal antibodies that have been validated for flow cytometry, IP, IF, IHC, ICC, and WB.

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Why Choose
Rabbit Monoclonal
Antibodies?

Rabbit monoclonal antibodies have been widely acknowledged for its high specificity and affinity. What enables rabbit monoclonal antibodies to be superior antibodies are the unique features of the rabbit immune system.

The rabbit immune system can produce greater antibody diversity due to the factors below.

Somatic Gene Conversion
& Somatic Hypermutation

Somatic Gene Conversion
& Somatic Hypermutation

Somatic gene conversion and hypermutation influence the variable regions of immunoglobulin (Ig) genes, which happen more often in rabbits than rodents. This ultimately contributes to enhancing the rabbit monoclonal antibody repertoire.

IgG Vs IgM

IgG Vs IgM

Rabbits have more IgG as an isotype compared to mice which have a higher ratio of IgM. Because IgG has better specificity than IgM, rabbits have more potential to develop specific antibodies.

CDRs (Complementarity-determining regions)

CDRs (Complementarity-determining regions)

Since rabbit CDR3 sequences are longer and more heterogeneous, more sequence variation can occur.

Lower immunodominance

Lower immunodominance

Immunodominance refers to particular epitopes being more immunogenic than other epitopes of the same antigen, which causes the immune system to mainly generate antibodies against the dominantly immunogenic epitope. The rabbit’s immune system has lower immunodominance than mouse, which allows for more antibody diversity.

Larger number
of lymphocytes

Larger number
of lymphocytes

Rabbits have larger spleens and other immune organs than mice, which indicates that more lymphocytes (B cells) are available for producing antibodies.

Extended period
of affinity maturation

Extended period
of affinity maturation

After immunization, affinity maturation can persist for a longer period of time for rabbits than mice, resulting in higher affinity and specificity for rabbit monoclonal antibodies.

Rabbit Monoclonal
Antibody Production

The general stages of rabbit monoclonal antibody production consist of rabbit immunization, plasma cell isolation, cloning and expression, and screening and selection. The rabbit monoclonal antibodies produced from a single clone are characterized for specific applications (e.g., ELISA, WB, IHC, etc.) and the best clone is chosen for scaling up production and performing purification. Because of the expensive and time-consuming process (usually 4-6 months) of monoclonal antibody production, they are typically more expensive than polyclonal antibodies.

Immunize rabbits

Isolate rabbit spleen cells

Fuse with myeloma partner cells

Recover hybridoma cells

Screen for monospecific clones

Produce best antibodies on small scales

Characterize antibodies by application

Scale up production and purification

Protocol Overview

Immunohistochemistry (IHC-P)

  • Tissue Preparation
  • Deparaffinization
  • Inactivation
  • Antigen Retrieval
  • Blocking
  • Primary Antibody Incubation
  • Secondary Antibody Incubation
  • Staining
View IHC-P & IHC-F Protocols

Western Blot

  • Protein Extraction
  • Protein Quantitation Assay
  • Gel Electrophoresis
  • Protein Transfer
  • Blocking
  • Primary Antibody Incubation
  • Secondary Antibody Incubation
  • Chromogenic or Chemiluminescent Detection
View WB Protocol

Flow Cytometry

  • Direct immunostaining of surface antigens
  • Indirect immunostaining of surface antigens
  • General immuno-staining procedure for intracellular antigens
  • Intracellular cytokine/phospho-immunostaining
  • In Vitro Cell Stimulation Reference Table
  • Dye efflux staining
  • DNA content or Cell cycle analysis
View Flow Cytometry Protocol

Q: Does BosterBio manufacture antibodies in-house?

A: Yes. We have over 26 years of experience in developing antibodies and ELISA kits with our proprietary platform. Our antibodies and ELISA kits are manufactured in-house and rigorously tested to ensure high quality so that our products will work as expected for your experiments.

Q: Will BosterBio update the antibody datasheet and/or COA if there are any changes?

A: Our online datasheets and COAs are not lot-specific. We provide the specific datasheets and COAs when we ship out antibodies. If you would like to request a lot-specific datasheet and/or COA, please contact [email protected] and provide the lot number.

Q: Can I get a trial sample of the rabbit monoclonal antibody?

A: If available, an option for a 30ug trial sample size will be shown on the product page.

Q: Can I get a discount for BosterBio antibodies?

A: We generally provide discounts for bulk orders. We also offer a “Buy Primary Antibody, Get Free HRP or Biotin Secondary for Free” promotion.

Q: Which types of antibodies does BosterBio provide? How do I choose the right antibody for my experiment?

A: We provide primary antibodies and secondary antibodies. Our primary antibodies include rabbit polyclonal antibodies, rabbit monoclonal antibodies, and mouse monoclonal antibodies. We also offer several types of unconjugated and conjugated secondary antibodies. Selecting the right antibody is dependent on the application, specificity, cross-reactivity, species, and immunogen required for your experiment. If you have special conjugation requests or need any technical assistance, please contact [email protected].

Q: Can BosterBio antibodies be used for multiple applications?

A: Our antibodies can be used for the validated applications listed on the product page. If the antibody does not work for a validated application shown on the product page, please contact [email protected]. Our Boster Guarantee states that if our technicians are unable to make your experiment work, we will offer a refund or replacement.

Q: Will the antibody work for an application that is not listed on the product page?

A: Although an antibody may work for an application not shown on the product page, it is not covered by our Boster Guarantee. You can contact [email protected] to check if our lab has any data for the requested application.

Q: Is the immunogen sequence provided?

A: We provide the immunogen sequence for most of our antibodies. If the immunogen sequence is not listed, please contact the [email protected] to check if the information can be shared.

Q: What is the antibody clone ID? What is the difference between the clone ID and the lot number?

A: The antibody clone ID is a unique identity number assigned to each monoclonal antibody clone. The lot number is the identifier for each batch of antibodies produced.

Q: Can BosterBio develop a custom rabbit monoclonal antibody for me if I do not find a suitable rabbit monoclonal antibody from the catalog?

A: If you cannot find a suitable rabbit monoclonal antibody from our catalog, please feel free to view details of our custom rabbit monoclonal antibody production service and contact us for a free consultation.

Q: If the antibody does not work, can I request a replacement or refund?

A: If the antibody does not work for an application we have listed on the product page, it is covered by our Boster Guarantee. You can contact [email protected] for technical troubleshooting. If our technicians are unable to help make your experiment work, we will provide a replacement or refund.

Q: What is the recommended concentration I should use for the primary antibody? How do I decide the suitable antibody dilution?

A: Recommended dilutions are listed on our product datasheets. However, the end user will need to determine the optimal dilution ratios because of different detection devices, methods, samples, and other factors.

Q: Why is the actual band size different from the predicted band size for my western blot?

A: Please see this blog for possible reasons. If you have any questions, please contact [email protected].

Q: When can I receive the products after I place an order?

A: For US customers, the lead time is typically 7-10 business days after you have placed your order. For international customers, please contact your distributor for time estimates. Custom rabbit monoclonal antibodies follow a different timeline. Please visit our custom rabbit monoclonal antibody service page and contact us for more details about the service and production timeline.

Q: How do you ship antibodies?

A: We ship antibodies through FedEx Priority and provide the tracking number to you. The package will include wet ice.

Q: How should I aliquot the antibodies?

A: Aliquot volumes are dependent on the amount needed for your experiment. However, we recommend aliquots should 10μL or more. If an aliquot has too little volume, antibody concentration may be affected by liquid evaporation and/or container adsorption.

Q: How should I store the antibodies? What is the shelf life of BosterBio's antibodies?

A: Our rabbit monoclonal antibodies can be stored at -20°C for one year. After reconstitution, the rabbit monoclonal antibody should be stored at 4°C for one month. If aliquotted, the antibody can be stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.