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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human Endothelin 1/EDN1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Endothelin 1/EDN1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human EDN1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Endothelin 1/EDN1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0945)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: human peptide; Immunogen sequence: C53-W73
3.9 pg/ml - 250 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0945 is reactive to EDN1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0945 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Endothelin-1
The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.
|EK0945-CAP||Anti-Human EDN1 Pre-coated 96-well strip microplate||1|
|EK0945-ST||Human EDN1 Standard||2 vials, 1 ng/tube|
|EK0945-DA||Human EDN1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Endothelin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Endothelin 1/EDN1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Endothelin-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For EDN1 (Source: Uniprot.Org, NCBI)
ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME EDN1 ARCND3, ET1, HDLCQ7, PPET1, QME endothelin 1 endothelin-1|preproendothelin-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on EDN1, check out the EDN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EDN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0945 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Peng Hj, Dai Dz, Ji H, Dai Y. Eur J Pharmacol. 2010 May 25;634(1-3):101-6. Doi: 10.1016/J.Ejphar.2010.02.001. Epub 2010 Feb 16. The Separate Roles Of Endothelin Receptors Participate In Remodeling Of Matrix Metalloproteinase And Connexin 43 Of Car...
Liu B, Zhu Y, Zhou J, Wei Y, Long C, Chen M, Ling Y, Ge J, Zhuo Y. Cell Stress Chaperones. 2014 Nov;19(6):827-35. Doi: 10.1007/S12192-014-0506-7. Epub 2014 Mar 19. Endoplasmic Reticulum Stress Promotes Amyloid-Beta Peptides Production In Rgc-5 Cells.
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