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Product Info Summary
| SKU: | FEK1313 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1313
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1313)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T40-R217
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1313 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1313 is reactive to Fcgr2b/Fcgr3 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1313 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Fcgr2b/Fcgr3
CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1313-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1313-ST | Mouse Fcgr2b/Fcgr3 Standard | 2 | 10 ng/tube |
| FEK1313-A | Mouse Fcgr2b/Fcgr3 Antibody A | 1 | 3ml |
| FEK1313-B | Mouse Fcgr2b/Fcgr3 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.073 | 0.167 | 0.225 | 0.369 | 0.639 | 1.107 | 1.793 | 2.400 |
Data Example Images
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Mouse Fcgr2b/Fcgr3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 88 | 208 | 904 | 96 | 234 | 841 |
| Standard deviation | 3.96 | 12.9 | 29.83 | 5.38 | 14.27 | 58.03 |
| CV (%) | 4.5% | 6.2% | 3.3% | 5.6% | 6.1% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Fcgr2b/Fcgr3 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 88 | 87 | 94 | 85 | 89 | 4.69 | 5.3% |
| Sample 2 | 208 | 235 | 216 | 244 | 226 | 11.06 | 4.9% |
| Sample 3 | 904 | 867 | 905 | 841 | 879 | 39.57 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For Fcgr2b/Fcgr3 (Source: Uniprot.Org, NCBI)
Gene Name
Fcgr2b/Fcgr3
Full Name
Weight
For more info on Fcgr2b/Fcgr3, check out the Fcgr2b/Fcgr3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fcgr2b/Fcgr3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit (FEK1313)
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