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Product Info Summary
| SKU: | EK0466 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin). |
Product info
Product Name
Mouse MMP-9 ELISA Kit PicoKine®
SKU/Catalog Number
EK0466
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MMP-9 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp9 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse MMP-9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0466)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: A20-P730
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0466 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0466 is reactive to MMP9 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin).
Application Guarantee
EK0466 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-9
The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0466-CAP | Anti-Mouse MMP9 Pre-coated 96-well strip microplate | 1 |
| EK0466-ST | Mouse MMP9 Standard | 2 vials, 10 ng/tube |
| EK0466-DA | Mouse MMP9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.022 | 0.056 | 0.116 | 0.199 | 0.362 | 0.681 | 1.250 | 1.995 |
Data Example Images
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Mouse MMP-9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-9, Dilution ratio of 1:200, concentration in serum and plasma is 150-400 ng/ml..
Some articles we found to cite concentrations of MMP-9 in samples: 12507913 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MMP-9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 205 | 1787 | 4289 | 222 | 1732 | 4214 |
| Standard deviation | 15.37 | 141.17 | 214.45 | 18.64 | 143.75 | 214.91 |
| CV (%) | 7.5% | 7.9% | 5% | 8.4% | 8.3% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 205 | 213 | 210 | 203 | 207 | 3.96 | 1.9% |
| Sample 2 | 1787 | 1777 | 1813 | 1705 | 1770 | 40.03 | 2.2% |
| Sample 3 | 4289 | 3856 | 3877 | 4024 | 4011 | 172.79 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For MMP9 (Source: Uniprot.Org, NCBI)
Gene Name
MMP9
Full Name
Matrix metalloproteinase-9
Weight
78.458kDa
Superfamily
peptidase M10A family
Alternative Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase MMP9 CLG4B, GELB, MANDP2, MMP-9 matrix metallopeptidase 9 matrix metalloproteinase-9|macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MMP9, check out the MMP9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse MMP-9 ELISA Kit PicoKine® (EK0466)
Hello CJ!
EK0466 has been cited in 52 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Suppression of murine B-cell lymphoma growth by trichosanthin through anti-angiogenesis
Marsdenia tenacissima extract suppresses tumor growth and angiogenesis in A20 mouse lymphoma
Urotensin II contributes to the formation of lung adenocarcinoma inflammatory microenvironment through the NF-κB pathway in tumor-bearing nude mice
Protective effects of rutin on lipopolysaccharide-induced heart injury in mice
The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression
Immune‐mediated ECM depletion improves tumour perfusion and payload delivery
IL-22-producing Th22 cells play a protective role in CVB3-induced chronic myocarditis and dilated cardiomyopathy by inhibiting myocardial fibrosis
Comparative mouse lung injury by nickel nanoparticles with differential surface modification
Shp2 positively regulates cigarette smoke-induced epithelial mesenchymal transition by mediating MMP-9 production
Evaluation on biological compatibility of carboxymethyl chitosan as biomaterials for antitumor drug delivery:
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
12 Customer Q&As for Mouse MMP-9 ELISA Kit PicoKine®
Question
Q: Can MMP-9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-12-02
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-12-02
Question
Are protease inhibitors needed when using AR0103 for EK0466 on mouse skin and liver samples? Keywords: Mammal Cell Protein Extraction Reagent
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
Liver tissue is not recommended for EK0466 due to the non-specific binding which may result from the high concentration of endogenous biotin. If the samples are freshly prepared and will be used shortly after, there is no need for protease inhibitors. Please include protease inhibitors if the samples will be stored for a long time before use.
Boster Scientific Support
Answered: 2020-03-04
Question
What is the immunized animal of the EK0466 kit and how is it purified?
Verified customer
Asked: 2019-08-13
Answer
Both capture and detection antibody of the Mouse MMP-9 ELISA Kit PicoKine EK0466 is goat and affinity purified.
Boster Scientific Support
Answered: 2019-08-15
Question
What is the protein source of the Avidin-Biotin-Peroxidase Complex(ABC) in the EK0466 kit and what are its other components that contain protein?
Verified customer
Asked: 2019-08-13
Answer
The Avidin and Peroxidase in the Mouse MMP-9 ELISA Kit PicoKine EK0466 were sourced from natural protein. The coated plate, detection antibody, sample diluent buffer, antibody diluent buffer and ABC diluent buffer contain BSA.
Boster Scientific Support
Answered: 2019-08-15
Question
Does the EK0466 kit contain human materials?
Verified customer
Asked: 2019-08-13
Answer
The Mouse MMP-9 ELISA Kit PicoKine EK0466 does not contain any human materials.
Boster Scientific Support
Answered: 2019-08-15
Question
Q: is it okay to use EDTA plasma as samples in Mouse MMP-9 Picokine® ELISA Kit (Catalog # EK0466)?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of MMP-9 causing disruption of its protein structure. MMP-9 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MMP-9 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-07-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-11-28
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of MMP-9 in Mouse plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-08-19
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MMP-9 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-08-19
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
W. Carter
Verified customer
Asked: 2018-07-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-07-16
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-12-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-12-27
Question
Q: how to analyze ELISA data? I have obtained MMP-9 level in plasma.
B. Turner
Verified customer
Asked: 2016-06-28
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-06-28
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
K. Surve
Verified customer
Asked: 2014-09-06
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-09-06
