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Mouse MMP-9 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MMP-9. 96wells/kit, with removable strips. Cited in 26 publication(s).
Mouse MMP-9 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0466 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernates, serum and plasma( heparin). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <20pg/ml |
| Assay Range: | 156pg/ml-10,000pg/ml |
Overview
| Product Name | Mouse MMP-9 ELISA Kit PicoKine™
See all Mmp9 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0466 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Mouse MMP9 with <20pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. MMP9 tissue specificity: |
| Cite This Product | Mouse MMP-9 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0466) |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma( heparin). |
| Sensitivity | <20pg/ml |
| Assay Range | 156pg/ml-10,000pg/ml |
| Immunogen | Expression system for standard: CHO; Immunogen sequence: A20-P730 |
| Antibody Clonalities | Capture antibody|Detection antibody polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P41245 |
|---|---|
| Gene Name | Mmp9 |
| Protein Name | Matrix metalloproteinase-9 |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix . |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity). . |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Mouse MMP-9 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0466-CAP | 96-well plate precoated with anti-Mouse Mmp9 antibody | 1 |
| EK0466-ST | lyophilized recombinant Mouse Mmp9 standard | 10ng/tube |
| EK0466-DA | biotinylated anti-Mouse Mmp9 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0466 Mouse MMP-9 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.022 | 0.116 | 0.199 | 0.362 | 0.681 | 1.250 | 1.995 | 2.428 |
We measured random samples of Mouse MMP-9 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 205 | 1787 | 4289 | 222 | 1732 | 4214 |
| Standard deviation | 15.37 | 141.17 | 214.45 | 18.64 | 143.75 | 214.91 |
| CV(%) | 7.5% | 7.9% | 5% | 8.4% | 8.3% | 5.1% |
We measured the performance consistency of Mouse MMP-9 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 205 | 213 | 210 | 203 | 207 | 3.96 | 1.9% |
| Sample 2 | 1787 | 1777 | 1813 | 1705 | 1770 | 40.03 | 2.2% |
| Sample 3 | 4289 | 3856 | 3877 | 4024 | 4011 | 172.79 | 4.3% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 26
| Xu J, Liu H, Wu Y, Gong X, Zhou Q, Qiao F. J Obstet Gynaecol Res. 2011 Mar;37(3):187-94. Doi: 10.1111/J.1447-0756.2010.01334.X. Epub 2010 Dec 16. Proapoptotic Effect Of Metalloproteinase 9 Secreted By Trophoblasts On Endothelial Cells. | PubMed ID 21159039 |
| Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regula... | PubMed ID 26113783 |
| Kang X, Li Y, Wei J, Zhang Y, Bian C, Wang K, Wu X, Hu Y, Li J, Yang Y. Plos One. 2013 Nov 1;8(11):E77427. Doi: 10.1371/Journal.Pone.0077427. Ecollection 2013. Elevation Of Matrix Metalloproteinase-9 Level In Cerebrospinal Fluid Of Tick-Borne Ence... | PubMed ID 24223711 |
| Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Bear... | PubMed ID 23226801 |
| Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes. | PubMed ID 24798055 |
| Stio M, Martinesi M, Treves C, Borgioli F. J Mater Sci Mater Med. 2015 Feb;26(2):100. Doi: 10.1007/S10856-015-5446-Y. Epub 2015 Feb 6. In Vitro Response Of Human Peripheral Blood Mononuclear Cells To Aisi 316L Austenitic Stainless Steel Subjected ... | PubMed ID 25655502 |
| Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation. | PubMed ID 21132400 |
| Pan B, Ren H, Ma Y, Liu D, Yu B, Ji L, Pan L, Li J, Yang L, Lv X, Shen X, Chen B, Zhang Y, Willard B, He Y, Zheng L. Int J Cancer. 2012 Jul 1;131(1):70-82. Doi: 10.1002/Ijc.26341. Epub 2011 Aug 30. High-Density Lipoprotein Of Patients With Type 2 ... | PubMed ID 21805479 |
| Liu Q,, Zou R, Zhou R, Gong C, Wang Z, Cai T, Tan C, Fang J. J Cell Biochem. 2015 Jul;116(7):1213-21. Doi: 10.1002/Jcb.25073. Mir-155 Regulates Glioma Cells Invasion And Chemosensitivity By P38 Isforms In Vitro. | PubMed ID 25535908 |
| Mazani M, Fard As, Baghi An, Nemati A, Mogadam Ra. J Pak Med Assoc. 2014 Jul;64(7):785-90. Effect Of Pomegranate Juice Supplementation On Matrix Metalloproteinases 2 And 9 Following Exhaustive Exercise In Young Healthy Males. | PubMed ID 25255587 |
| Yuan H, Yang P, Zhou D, Gao W, Qiu Z, Fang F, Ding S, Xiao W. Mol Biol Rep. 2014 Aug;41(8):5157-65. Doi: 10.1007/S11033-014-3382-4. Epub 2014 May 10. Knockdown Of Sphingosine Kinase 1 Inhibits The Migration And Invasion Of Human Rheumatoid Arthrit... | PubMed ID 24816639 |
| Tang Xp, Tang Gd, Fang Cy, Liang Zh, Zhang Ly. World J Gastroenterol. 2013 Mar 14;19(10):1582-92. Doi: 10.3748/Wjg.V19.I10.1582. Effects Of Ginsenoside Rh2 On Growth And Migration Of Pancreatic Cancer Cells. | PubMed ID 23538603 |
| Yuan M, Zhan Q, Duan X, Song B, Zeng S, Chen X, Yang Q, Xia J. Atherosclerosis. 2013 Feb;226(2):447-52. Doi: 10.1016/J.Atherosclerosis.2012.11.026. Epub 2012 Dec 8. A Functional Polymorphism At Mir-491-5P Binding Site In The 3'-Utr Of Mmp-9 Gene C... | PubMed ID 23257658 |
| Tan Sy, Wang Jy, Shen L, Luo Hs, Shen Zx. World J Gastroenterol. 2007 Apr 14;13(14):2108-12. Relationship Between Preoperative Staging By Endoscopic Ultrasonography And Mmp-9 Expression In Gastric Carcinoma. | PubMed ID 17465457 |
| Chen Sz, Yao Hq, Zhu Sz, Li Qy, Guo Gh, Yu J. Oncol Lett. 2015 Feb;9(2):915-919. Epub 2014 Dec 4. Expression Levels Of Matrix Metalloproteinase-9 In Human Gastric Carcinoma. | PubMed ID 25621068 |
| Jiang Z, Han B, Li H, Li X, Yang Y, Liu W. Carbohydr Polym. 2015 Jul 10;125:53-60. Doi: 10.1016/J.Carbpol.2015.02.039. Epub 2015 Feb 26. Preparation And Anti-Tumor Metastasis Of Carboxymethyl Chitosan. | PubMed ID 25857959 |
| You C, Zu J, Liu X, Kong P, Song C, Wei R, Zhou C, Wang Y, Yan J. J Cell Mol Med. 2018 Apr;22(4):2449-2457. doi: 10.1111/jcmm.13549. Epub 2018 Jan 30. Synovial fibroblast‐targeting liposomes encapsulating an NF‐κB‐blocking peptide ameliorates zymo... | PubMed ID 29383874 |
| Chen Y, Zeng C, Zhan Y, Wang H, Jiang X, Li W. Oncogene. 2017 Aug 17;36(33):4692-4705. doi: 10.1038/onc.2017.100. Epub 2017 Apr 10. Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-media... | PubMed ID 28394344 |
| Zou J, Wang N, Liu M, Bai Y, Wang H, Liu K, Zhang H, Xiao X, Wang K. J Cell Mol Med. 2018 May;22(5):2692-2705. doi: 10.1111/jcmm.13552. Epub 2018 Mar 7. Nucleolin mediated pro | PubMed ID 29512890 |
| Role of Sphingosine Kinase/S1P Axis in ECM Remodeling of Cardiac Cells Elicited by Relaxin | PubMed ID 25415609 |
| IL-22-producing Th22 cells play a protective role in CVB3-induced chronic myocarditis and dilated cardiomyopathy by inhibiting myocardial fibrosis | PubMed ID 25547181 |
| Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection | PubMed ID 26126526 |
| MMP-9-Dependent Serum-Borne Bioactivity Caused by Multiwalled Carbon Nanotube Exposure Induces Vascular Dysfunction via the CD36 Scavenger Receptor | PubMed ID 26801584 |
| Marsdenia tenacissima extract suppresses tumor growth and angiogenesis in A20 mouse lymphoma | PubMed ID 28521395 |
| Association of NF-%u03BAB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes | PubMed ID 30087244 |
| Involvement of CD74 in head and neck squamous cell carcinomas | PubMed ID 24663824 |
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Questions and answers from customers related to EK0466 Mouse MMP-9 ELISA Kit PicoKine™
2 Related Questions
Question
Q: Are protease inhibitors needed when using AR0103 for EK0466 on mouse skin and liver samples? Keywords: Mammal Cell Protein Extraction Reagent
Verified Customer
Asked: 0000-00-00
Answer
A: Liver tissue is not recommended for EK0466 due to the non-specific binding which may result from the high concentration of endogenous biotin. If the samples are freshly prepared and will be used shortly after, there is no need for protease inhibitors. Please include protease inhibitors if the samples will be stored for a long time before use.
Boster Scientific Support
Answered: 0000-00-00
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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