Human MMP-9 ELISA Kit PicoKine®

Product Name

Human MMP-9 ELISA Kit PicoKine®

SKU/Catalog Number

EK0465

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

MMP9 (gelatinase B) is a secreted zinc metalloprotease; the page notes MMPs degrade extracellular matrix, enabling angiogenesis and tumor invasion (ECM remodeling axis). Assay context: quantitative ELISA with validated matrices including cell culture supernatants, serum, plasma (heparin), saliva, and urine; sensitivity <5 pg/mL and assay range 156–10,000 pg/mL, supporting soluble protease biomarker quantitation (research use). Often profiled alongside other MMPs such as MMP3 and pro-angiogenic/tumor microenvironment factors such as ANGPTL4 to separate ECM remodeling from hypoxia/anoikis programs (interpretive, putative); confirmatory orthogonal measurements may include WB or multiplex IHC depending on sample type.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human MMP-9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0465)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: A20-D707

Sensitivity

<5 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0465 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0465 is reactive to MMP9 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Application Guarantee

EK0465 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of MMP9

The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.

Kit Components

Catalog Number	Description	Quantity
EK0465-CAP	Anti-Human MMP9 Pre-coated 96-well strip microplate	1
EK0465-ST	Human MMP9 Standard	2 vials, 10 ng/tube
EK0465-DA	Human MMP9 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.031	0.094	0.177	0.315	0.587	1.032	1.701	2.469

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MMP9, For serum, dilution ratio is 1:200 and concentration is 135.2-846 ng/ml.
For plasma, dilution ratio is 1:20 and concentration is 10.56-126 ng/ml..

Some articles we found to cite concentrations of MMP9 in samples: 25469360 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MMP-9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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