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Product Info Summary
| SKU: | EK0465 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin) , saliva and urine. |
Product info
Product Name
Human MMP-9 ELISA Kit PicoKine®
SKU/Catalog Number
EK0465
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MMP-9 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP9 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MMP-9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0465)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A20-D707
Sensitivity
<5 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0465 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0465 is reactive to MMP9 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) , saliva and urine.
Application Guarantee
EK0465 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-9
The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0465-CAP | Anti-Human MMP9 Pre-coated 96-well strip microplate | 1 |
| EK0465-ST | Human MMP9 Standard | 2 vials, 10 ng/tube |
| EK0465-DA | Human MMP9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.051 | 0.117 | 0.194 | 0.296 | 0.525 | 0.919 | 1.523 | 1.982 |
Data Example Images
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Human MMP-9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-9, For serum, dilution ratio is 1:200 and concentration is 135.2-846 ng/ml.
For plasma, dilution ratio is 1:20 and concentration is 10.56-126 ng/ml..
Some articles we found to cite concentrations of MMP-9 in samples: 25469360 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 237 | 1530 | 5547 | 250 | 1429 | 5731 |
| Standard deviation | 18.72 | 108.63 | 439.41 | 23.5 | 135.75 | 504.32 |
| CV (%) | 7.9% | 7.1% | 7.9% | 9.4% | 9.5% | 8.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 237 | 203 | 233 | 214 | 221 | 13.88 | 6.2% |
| Sample 2 | 1530 | 1745 | 1683 | 1555 | 1628 | 88.94 | 5.4% |
| Sample 3 | 5547 | 5421 | 5245 | 5758 | 5492 | 186.96 | 3.4% |
Protein Target Info & Infographic
Gene/Protein Information For MMP9 (Source: Uniprot.Org, NCBI)
Gene Name
MMP9
Full Name
Matrix metalloproteinase-9
Weight
78.458kDa
Superfamily
peptidase M10A family
Alternative Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase MMP9 CLG4B, GELB, MANDP2, MMP-9 matrix metallopeptidase 9 matrix metalloproteinase-9|macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MMP9, check out the MMP9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MMP-9 ELISA Kit PicoKine® (EK0465)
Hello CJ!
EK0465 has been cited in 75 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts
Expression levels of matrix metalloproteinase-9 in human gastric carcinoma
Calcium‑sensing receptor promotes high glucose‑induced myocardial fibrosis via upregulation of the TGF‑β1/Smads pathway in cardiac fibroblasts
LPS‑induced proinflammatory cytokine expression in human airway epithelial cells and macrophages via NF‑κB, STAT3 or AP‑1 activation
Sinomenine prevents metastasis of human osteosarcoma cells via S phase arrest and suppression of tumor-related neovascularization and osteolysis through the CXCR4-STAT3 pathway
FOXP3 facilitates the invasion and metastasis of non‑small cell lung cancer cells through regulating VEGF, EMT and the Notch1/Hes1 pathway
Cyclin G2 Inhibits Oral Squamous Cell Carcinoma Growth and Metastasis by Binding to IGFBP3 and Regulating the FAK-SRC-STAT Signaling Pathway
Correlation analysis of levels of adiponectin and matrix metalloproteinase-9 with stability of coronary heart disease
Gelatinases and their tissue inhibitors in a group of subjects with obstructive sleep apnea syndrome
Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
8 Customer Q&As for Human MMP-9 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Mitchell
Verified customer
Asked: 2020-10-03
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-10-03
Question
Q: Can MMP-9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-08-08
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-08-08
Question
Q: is it okay to use EDTA plasma as samples in Human MMP-9 Picokine® ELISA Kit (Catalog # EK0465)?
T. Carter
Verified customer
Asked: 2020-07-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of MMP-9 causing degradation of its protein structure. MMP-9 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the MMP-9 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-07-30
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-08-09
Question
Can EK0465 be used on human tears sample?
Verified customer
Asked: 2019-07-02
Answer
Our lab technicians haven't worked on human tear sample using the Human MMP-9 ELISA Kit PicoKine™ (EK0465). Please run a pilot test.
Boster Scientific Support
Answered: 2019-07-03
Question
Q: if the enzyme conjugated MMP-9 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-9 antigen?
T. Hill
Verified customer
Asked: 2019-01-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-01-06
Question
For my reference, what is the normal value of MMP-9? Keyword: data, results
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
The normal value of MMP-9 in human serum is about 173~698 ng/ml, that in human plasma is about 13.9~104 ng/ml.
Boster Scientific Support
Answered: 2018-05-22
Question
Does EK0465 detect both the secreted and active form of MMP9? Keywords: secreted form, active form, MMP9, detection
Verified Customer
Verified customer
Asked: 2013-02-25
Answer
Yes, Boster’s EK0465 is able to detect both the secreted and active form of MMP9.
Boster Scientific Support
Answered: 2013-02-25
