Human MMP-9 ELISA Kit PicoKine™

Human MMP-9 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP9 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 5pg/ml. Cited in 37 publication(s).

Product Info Summary

SKU: EK0465
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Product Name

Human MMP-9 ELISA Kit PicoKine™

See all MMP-9 products

SKU/Catalog Number

EK0465

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MMP-9 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP9 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MMP-9 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0465)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-D707

Sensitivity

<5 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0465 is reactive to MMP9 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Application Guarantee

EK0465 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0465-CAP Anti-Human MMP9 Pre-coated 96-well strip microplate 1
EK0465-ST Human MMP9 Standard 2 vials, 10 ng/tube
EK0465-DA Human MMP9 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0510.1170.1940.2960.5250.9191.5231.982

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MMP-9 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2371530554725014295731
Standard deviation18.72108.63439.4123.5135.75504.32
CV (%)7.9%7.1%7.9%9.4%9.5%8.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-9 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 123720323321422113.886.2%
Sample 21530174516831555162888.945.4%
Sample 355475421524557585492186.963.4%
*number of samples for each test n=16.

Gene/Protein Information For MMP9 (Source: Uniprot.Org, NCBI)

Uniprot ID

P14780

Gene ID

4318

Gene Name

MMP9

Full Name

Matrix metalloproteinase-9

Weight

78.458kDa

Superfamily

peptidase M10A family

Alternative Names

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase MMP9 CLG4B, GELB, MANDP2, MMP-9 matrix metallopeptidase 9 matrix metalloproteinase-9|macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP9, check out the MMP9 Infographic

MMP9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0465 has been cited in 37 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang H,Yuan M,Yang E,Chen D,Su A,Wu Z.Enterovirus 71 infection induced Aquaporin-4 depolarization by increasing matrix metalloproteinase-9 activity.Neurosci Lett.2021 Jun 11:136049.doi:10.1016/j.neulet.2021.136049.Epub ahead of print.PMID:34126180.
Species: Human,Mouse
EK0465 usage in article: APP:ELISA, SAMPLE:CSF, DILUTION:NA

Cosentino G,Di Stefano V,Presti RL,Montana M,Todisco M,Gastaldi M,Cortese A,Alfonsi E,Tassorelli C,Fierro B,Caimi G,Brighina F.Expression pattern of matrix metalloproteinases-2 and -9 and their tissue inhibitors in patients with chronic inflammatory demyelinating polyneuropathy.Neurol Sci.2021 May 14.doi:10.1007/s10072-021-05314-y.Epub ahead of print.PMID:33988800.
Species: Human
EK0465 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Shuguo Yuan,Wan Li,Liangqiu Tang et al.MMP-9 in Myocardial Fibrosis and Structural Remodeling in Rheumatic Heart Disease Patients with Atrial Fibrillation: A Single-center Prospective Case-control Study, 18 January 2021, PREPRINT (Version 1) available at
Species: Human
EK0465 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Singh A,Banerjee T,Shukla SK.Factors Associated with High Rates of Recurrence of Amebic Liver Abscess (ALA) in North India.Am J Trop Med Hyg.2021 Jan 11.doi:10.4269/ajtmh.20-0074.Epub ahead of print.PMID:33432901.
Species: Human
EK0465 usage in article: APP:ELISA, SAMPLE:LIVER ASPIRATE, DILUTION:NA

Liu YN,Guan Y,Shen J,Jia YL,Zhou JC,Sun Y,Jiang JX,Shen HJ,Shu Q,Xie QM,Xie Y. Shp2 positively regulates cigarette smoke-induced epithelial mesenchymal transition by mediating MMP-9 production. Respir Res. 2020 Jun 26;21(1):161.doi:10.1186/s12931-020-0142
Species: Human,Mouse

Hu S, Zheng W, Jin L. Astragaloside IV inhibits cell proliferation and metastasis of breast cancer via promoting the long noncoding RNA TRHDE-AS1. J Nat Med. 2020 Nov 20. doi: 10.1007/s11418-020-01469-8. Epub ahead of print. PMID: 33219447.
Species: Human,Mouse
EK0465 usage in article: APP:ELISA, SAMPLE:MCF-10A CELLS,MCF-7 CELLS,MDA-MB-231 CELLS AND MDA-MB-468 CELLS, DILUTION:NA

Galectin-1 suppression delineates a new strategy to inhibit myeloma-induced angiogenesis and tumoral growth in vivo

Correlation analysis of levels of adiponectin and matrix metalloproteinase-9 with stability of coronary heart disease

Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis

Precursor N-cadherin mediates glial cell line-derived neurotrophic factor-promoted human malignant glioma

miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?

Changes in Blood Components in Aphtha Patients with Excess Heat

Effects of resveratrol and genistein on nuclear factor??B, tumor necrosis factor?? and matrix metalloproteinase?9 in patients with chronic obstructive pulmonary disease

The Angiogenic Effect of microRNA-21 Targeting TIMP3 through the Regulation of MMP2 and MMP9

Increased expression of matrix metalloproteinase-9 associated with gastric ulcer recurrence

Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts

Sun Y, Chen Y, Li S, Lei Y, Xu D, Jiang N, Zhang Y, Cao J, Ke Z. Int J Nanomedicine. 2017 Aug 31;12:6399-6412. doi: 10.2147/IJN.S144033. eCollection 2017. NanoVelcro-captured CTC number concomitant with enhanced serum levels of MMP7 and MMP9 enabl...

Matin S, Nemati A, Ghobadi H, Alipanah-Moghadam R, Rezagholizadeh L. Int J Chron Obstruct Pulmon Dis. 2018 May 3;13:1449-1454. doi: 10.2147/COPD.S155985. eCollection 2018. The effect of conjugated linoleic acid on oxidative stress and matrix metal...

Sun Y, Yang D, Xi L, Chen Y, Fu L, Sun K, Yin J, Li X, Liu S, Qin Y, Liu M, Hou Y. Cell Death Dis. 2018 Jan 26;9(2):122. doi: 10.1038/s41419-017-0133-1. Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity cha...

Lin H, Qu Y, Geng Z, Li C, Wu H, Dong N, Liu Z, Li W. Plos One. 2014 Jan 31;9(1):E87368. Doi: 10.1371/Journal.Pone.0087368. Ecollection 2014. Air Exposure Induced Characteristics Of Dry Eye In Conjunctival Tissue Culture.

Chen Sz, Yao Hq, Zhu Sz, Li Qy, Guo Gh, Yu J. Oncol Lett. 2015 Feb;9(2):915-919. Epub 2014 Dec 4. Expression Levels Of Matrix Metalloproteinase-9 In Human Gastric Carcinoma.

Tan Sy, Wang Jy, Shen L, Luo Hs, Shen Zx. World J Gastroenterol. 2007 Apr 14;13(14):2108-12. Relationship Between Preoperative Staging By Endoscopic Ultrasonography And Mmp-9 Expression In Gastric Carcinoma.

Yuan M, Zhan Q, Duan X, Song B, Zeng S, Chen X, Yang Q, Xia J. Atherosclerosis. 2013 Feb;226(2):447-52. Doi: 10.1016/J.Atherosclerosis.2012.11.026. Epub 2012 Dec 8. A Functional Polymorphism At Mir-491-5P Binding Site In The 3'-Utr Of Mmp-9 Gene C...

Zawilla N, Taha F, Ibrahim Y. Int J Occup Environ Health. 2014 Apr-Jun;20(2):146-56. Doi: 10.1179/2049396714Y.0000000061. Epub 2014 Mar 24. Liver Functions In Silica-Exposed Workers In Egypt: Possible Role Of Matrix Remodeling And Immunological Fa...

Tang Xp, Tang Gd, Fang Cy, Liang Zh, Zhang Ly. World J Gastroenterol. 2013 Mar 14;19(10):1582-92. Doi: 10.3748/Wjg.V19.I10.1582. Effects Of Ginsenoside Rh2 On Growth And Migration Of Pancreatic Cancer Cells.

Yuan H, Yang P, Zhou D, Gao W, Qiu Z, Fang F, Ding S, Xiao W. Mol Biol Rep. 2014 Aug;41(8):5157-65. Doi: 10.1007/S11033-014-3382-4. Epub 2014 May 10. Knockdown Of Sphingosine Kinase 1 Inhibits The Migration And Invasion Of Human Rheumatoid Arthrit...

Mazani M, Fard As, Baghi An, Nemati A, Mogadam Ra. J Pak Med Assoc. 2014 Jul;64(7):785-90. Effect Of Pomegranate Juice Supplementation On Matrix Metalloproteinases 2 And 9 Following Exhaustive Exercise In Young Healthy Males.

Liu Q,, Zou R, Zhou R, Gong C, Wang Z, Cai T, Tan C, Fang J. J Cell Biochem. 2015 Jul;116(7):1213-21. Doi: 10.1002/Jcb.25073. Mir-155 Regulates Glioma Cells Invasion And Chemosensitivity By P38 Isforms In Vitro.

Pan B, Ren H, Ma Y, Liu D, Yu B, Ji L, Pan L, Li J, Yang L, Lv X, Shen X, Chen B, Zhang Y, Willard B, He Y, Zheng L. Int J Cancer. 2012 Jul 1;131(1):70-82. Doi: 10.1002/Ijc.26341. Epub 2011 Aug 30. High-Density Lipoprotein Of Patients With Type 2 ...

Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation.

Stio M, Martinesi M, Treves C, Borgioli F. J Mater Sci Mater Med. 2015 Feb;26(2):100. Doi: 10.1007/S10856-015-5446-Y. Epub 2015 Feb 6. In Vitro Response Of Human Peripheral Blood Mononuclear Cells To Aisi 316L Austenitic Stainless Steel Subjected ...

Hopps E, Lo Presti R, Montana M, Noto D, Averna Mr, Caimi G. J Investig Med. 2013 Aug;61(6):978-83. Doi: 10.231/Jim.0B013E318294E9Da. Gelatinases And Their Tissue Inhibitors In A Group Of Subjects With Metabolic Syndrome.

Hopps E, Lo Presti R, Montana M, Canino B, Averna Mr, Caimi G. Mediators Inflamm. 2014;2014:510619. Doi: 10.1155/2014/510619. Epub 2014 Jul 10. Study Of The Correlations Among Some Parameters Of The Oxidative Status, Gelatinases, And Their Inhibit...

Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes.

Kang X, Li Y, Wei J, Zhang Y, Bian C, Wang K, Wu X, Hu Y, Li J, Yang Y. Plos One. 2013 Nov 1;8(11):E77427. Doi: 10.1371/Journal.Pone.0077427. Ecollection 2013. Elevation Of Matrix Metalloproteinase-9 Level In Cerebrospinal Fluid Of Tick-Borne Ence...

Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regula...

Xu J, Liu H, Wu Y, Gong X, Zhou Q, Qiao F. J Obstet Gynaecol Res. 2011 Mar;37(3):187-94. Doi: 10.1111/J.1447-0756.2010.01334.X. Epub 2010 Dec 16. Proapoptotic Effect Of Metalloproteinase 9 Secreted By Trophoblasts On Endothelial Cells.

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8 Customer Q&As for Human MMP-9 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

H. Mitchell

Verified customer

Asked: 2020-10-03

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-10-03

Question

Q: Can MMP-9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-08-08

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-08-08

Question

Q: is it okay to use EDTA plasma as samples in Human MMP-9 Picokine® ELISA Kit (Catalog # EK0465)?

T. Carter

Verified customer

Asked: 2020-07-30

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of MMP-9 causing degradation of its protein structure. MMP-9 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the MMP-9 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-07-30

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-09

Question

Can EK0465 be used on human tears sample?

Verified customer

Asked: 2019-07-02

Answer

Our lab technicians haven't worked on human tear sample using the Human MMP-9 ELISA Kit PicoKine™ (EK0465). Please run a pilot test.

Boster Scientific Support

Answered: 2019-07-03

Question

Q: if the enzyme conjugated MMP-9 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-9 antigen?

T. Hill

Verified customer

Asked: 2019-01-06

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-01-06

Question

For my reference, what is the normal value of MMP-9? Keyword: data, results

Verified Customer

Verified customer

Asked: 2018-05-22

Answer

The normal value of MMP-9 in human serum is about 173~698 ng/ml, that in human plasma is about 13.9~104 ng/ml.

Boster Scientific Support

Answered: 2018-05-22

Question

Does EK0465 detect both the secreted and active form of MMP9? Keywords: secreted form, active form, MMP9, detection

Verified Customer

Verified customer

Asked: 2013-02-25

Answer

Yes, Boster’s EK0465 is able to detect both the secreted and active form of MMP9.

Boster Scientific Support

Answered: 2013-02-25

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