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Product Info Summary
| SKU: | PA1883 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband®
SKU/Catalog Number
PA1883
BA3850 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Flavin containing monooxygenase 4/FMO4 Antibody catalog # PA1883. Tested in ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1883)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Flavin containing monooxygenase 4, different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1883 is reactive to FMO4 in Human, Mouse, Rat
Observed Molecular Weight
63 kDa
Calculated molecular weight
63.3 kDa
Background of FMO4
FMO4 (Flavin-containing monooxygenase 4) also known as FMO2, FORMERLY or FMO, ADULT LIVER FORM, is an enzyme that in humans is encoded by the FMO4 gene. By PCR analysis, Dolphin et al. (1992) mapped the FMO4 gene to chromosome 1. Southern blot hybridization with single exon probes demonstrated that human FMO4 and FMO1 are, in each case, the product of a single gene. Lawton et al. (1994) proposed a reclassification of mammalian FMOs. Under this system, FMO2 described by Dolphin et al. (1992) is now designated FMO4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1883 is guaranteed for IF, ICC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat |
| Immunocytochemistry/Immunofluorescence | 5 μg/ml | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of FMO4 using anti-FMO4 antibody (PA1883).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO4 antigen affinity purified polyclonal antibody (PA1883) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FMO4 at approximately 63 kDa. The expected band size for FMO4 is at 63 kDa.
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IF analysis of FMO4 using anti-FMO4 antibody (PA1883).
FMO4 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FMO4 Antibody (PA1883) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband® (PA1883)
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4 Customer Q&As for Anti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband®
Question
We want to test anti-Flavin containing monooxygenase 4/FMO4 antibody PA1883 on mouse liver for research purposes, then I may be interested in using anti-Flavin containing monooxygenase 4/FMO4 antibody PA1883 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The products we sell, including anti-Flavin containing monooxygenase 4/FMO4 antibody PA1883, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-27
Question
We are currently using anti-Flavin containing monooxygenase 4/FMO4 antibody PA1883 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
The anti-Flavin containing monooxygenase 4/FMO4 antibody (PA1883) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-20
Question
Is this PA1883 anti-Flavin containing monooxygenase 4/FMO4 antibody reactive to the isotypes of FMO4?
R. Mangal
Verified customer
Asked: 2017-10-26
Answer
The immunogen of PA1883 anti-Flavin containing monooxygenase 4/FMO4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Flavin containing monooxygenase 4(75-92aa HEDYPNFMNHEKFWDYLQ), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-26
Question
Is a blocking peptide available for product anti-Flavin containing monooxygenase 4/FMO4 antibody (PA1883)?
L. Edwards
Verified customer
Asked: 2015-04-20
Answer
We do provide the blocking peptide for product anti-Flavin containing monooxygenase 4/FMO4 antibody (PA1883). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-04-20
