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Product Info Summary
| SKU: | A06270-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Intestinal Cell Kinase ICK Antibody
SKU/Catalog Number
A06270-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Intestinal Cell Kinase ICK Antibody catalog # A06270-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Intestinal Cell Kinase ICK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06270-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ICK. AA range:125-174
Reactive Species
A06270-1 is reactive to ICK in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
71.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06270-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200 (4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200
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Western Blot analysis of 3T3 cells using Intestinal Cell Kinase Polyclonal Antibody
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Western blot analysis of lysates from NIH/3T3 cells, treated with PBS 10uM 60', using ICK Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Intestinal Cell Kinase ICK Antibody (A06270-1)
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Customer Q&As
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5 Customer Q&As for Anti-Intestinal Cell Kinase ICK Antibody
Question
We are currently using anti-Intestinal Cell Kinase antibody A06270-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The anti-Intestinal Cell Kinase antibody (A06270-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-04
Question
Is there a BSA free version of anti-Intestinal Cell Kinase antibody A06270-1 available?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Intestinal Cell Kinase antibody A06270-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-19
Question
I was wanting to use your anti-Intestinal Cell Kinase antibody for WB for rat embl:aah35807.1} on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat embl:aah35807.1} identification?
T. Mitchell
Verified customer
Asked: 2019-11-12
Answer
It shows on the product datasheet, A06270-1 anti-Intestinal Cell Kinase antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat embl:aah35807.1} in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-12
Question
Is a blocking peptide available for product anti-Intestinal Cell Kinase antibody (A06270-1)?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We do provide the blocking peptide for product anti-Intestinal Cell Kinase antibody (A06270-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-02
Question
Can you help my question with product A06270-1, anti-Intestinal Cell Kinase antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06270-1 anti-Intestinal Cell Kinase antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-26
