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Product Info Summary
| SKU: | PA1687 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NLRP4G Antibody Picoband®
SKU/Catalog Number
PA1687
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NLRP4G Antibody catalog # PA1687. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NLRP4G Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1687)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse NLRP4G.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1687 is reactive to Nlrp4g in Mouse
Observed Molecular Weight
55 kDa
Background of Nlrp4g
Stra8, stimulated by retinoic acid 8, is expressed in embryonic ovaries just before meiotic initiation, whereas in testis it is expressed after birth. The STRA8 gene contains 9 exons. And this gene is mapped to chromosome 7q31. Retinoic acid (RA) signaling was required for Stra8 expression, and therefore initiation of meiosis, in embryonic mouse ovaries. RA was sufficient to induce Stra8 expression in embryonic testis. Cyp26-mediated RA metabolism was responsible for delaying Stra8 expression in embryonic testis. Since RA signaling regulated Stra8 expression in both embryonic ovaries and adult testis, it was said this portion of the meiotic initiation pathway may be identical in both sexes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1687 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Immunohistochemistry (Paraffin-embedded Section) | 0.5-1μg/ml | Mouse |
| Western blot | 0.1-0.5μg/ml | Mouse |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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Anti-NLRP4G antibody, PA1687, Western blotting
WB: Mouse Ovary Tissue Lysate
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IHC analysis of NLRP4G using anti-NLRP4G antibody (PA1687).
NLRP4G was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NLRP4G Antibody (PA1687) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-NLRP4G Antibody Picoband® (PA1687)
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Customer Q&As
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1 Customer Q&As for Anti-NLRP4G Antibody Picoband®
Question
We are currently using anti-NLRP4G antibody PA1687 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The anti-NLRP4G antibody (PA1687) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-24
