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This antibody is highly specific and efficient, suitable for Western blot detection of LYN protein in HT22 cells, yielding clear and well-defined bands.

Excellent, submitted by on
SKU A01424-2
Application Western Blot
Sample Mouse HT-22 cells
Sample Processing Description After digestion, cells were collected by centrifugation and lysed in 1 mL of RIPA lysis buffer supplemented with a protease inhibitor cocktail on ice for 1 h. The lysates were then centrifuged, and the supernatants were collected. After protein quantification, 5× loading buffer was added, and the samples were denatured in a 100 °C water bath for 10 min before loading for electrophoresis.
Other Reagents Blocking buffer
Primary Antibody Lyn Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The control represents HT22 cells without drug treatment, while Low, Medium, and High correspond to experimental groups treated with three different drug concentrations for 24 h. The Western blot results obtained with this antibody are clear and well-defined, allowing distinct comparison of expression levels between the groups. It can be observed that the expression of the target protein slightly increases with increasing drug concentration.

This antibody is highly specific and efficient, suitable for Western blot detection of ABCD3 protein in MARC-145 cells, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU PA2091
Application Western Blot
Sample Monkey MARC-145 cells
Sample Processing Description RIPA lysis buffer supplemented with protease inhibitor PMSF (100:1) was used to lyse the cells for 10 min. The lysates were then centrifuged at 12,000 rpm for 15 min, and the supernatants were collected. 5× protein loading buffer was added, and samples were denatured at 100 °C for 10 min before loading onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody PMP70/ABCD3 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for ABCD3 and the internal control Actin in MARC-145 cells under normal and toxin-treated conditions. The target bands are clear and well-defined, and the experimental results are satisfactory.

This antibody exhibits high specificity and efficiency and is suitable for Western blot detection of IL6 protein in MCF-7 cells, yielding clean and well-defined target bands.

Excellent, submitted by on
SKU PA1352
Application Western Blot
Sample Human mcf-7 cells
Sample Processing Description RIPA lysis buffer supplemented with a protease inhibitor cocktail was added to the cell culture dish, and the cells were lysed on ice for 30 min. The lysates were then centrifuged, and the supernatants were collected. Protein concentration was determined, and samples were adjusted to equal concentrations. Subsequently, 5× protein loading buffer was added, and the samples were denatured at 100 °C for 10 min. A total of 15 μL of protein sample was loaded per lane for SDS-PAGE electrophoresis.
Other Reagents Blocking buffer
Primary Antibody CRCP Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary As shown in the figure, the expression levels of CRCP protein in MCF-7 cells at different drug treatment time points are presented. The Western blot results obtained with this antibody are clear; although there are a few faint nonspecific bands above, they do not affect result interpretation. It can be observed that the expression of the target protein in MCF-7 cells increases with longer drug treatment times.

This antibody is highly specific and efficient, suitable for Western blot detection of Catalase protein in MARC-145 cells, with only minor nonspecific bands.

Excellent, submitted by on
SKU PB9925
Application Western Blot
Sample MARC-145 cells
Sample Processing Description Cells or tissues were lysed in RIPA buffer supplemented with protease inhibitor PMSF (100:1) for 10 minutes. The lysate was centrifuged at 12,000 rpm for 15 minutes, and the supernatant was collected. Samples were mixed with 5× loading buffer and denatured at 100 °C for 10 minutes before loading onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Catalase Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-rabbit IgG secondary antibody conjugated with horseradish peroxidase (HRP)
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for the target protein Catalase and the loading control Actin in MARC-145 cells under normal and post-infection conditions. The target bands are clear and well-defined, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for Western blot detection of Anti-SYK Antibody protein in HT22 cells, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU A00318-1
Application Western Blot
Sample Mouse hippocampal tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing protease inhibitors at 4°C for 2 hours, followed by centrifugation to collect the supernatant. Protein concentration was then determined, and after adjusting to the desired concentration, samples were mixed with 5× protein loading buffer and denatured by heating for 10 minutes. Fifteen microliters of each sample were loaded per lane for electrophoresis.
Other Reagents Blocking buffer
Primary Antibody PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic of the WB results for PIK3R1 and the loading control β-actin in brain tissues from normal mice, model mouse, and mouse treated with high and low doses of AB drug. Although the expression differences between experimental groups are not obvious, the WB results with this antibody are still clear and well-defined.

This antibody demonstrates good specificity, with minimal nonspecific bands. The target band is clear and at the correct position, and expression differences between different groups are observable.

Excellent, submitted by on
SKU M00297
Application Western Blot
Sample Mouse hippocampal tissue
Sample Processing Description An Alzheimer’s disease (AD) model was established by injecting streptozotocin (STZ) into the lateral ventricles of mice. Hippocampal tissues were collected after treatment with four different doses of QianCengTa compound, and total protein was extracted.
Other Reagents RIPA lysis buffer,Protease inhibitor,Electrophoresis buffer,Transfer buffer,Blocking buffer
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary c-FOS is a marker of neuronal activation under pathological conditions. Its expression is low in normal brain tissue but elevated in the AD model, and decreases following drug treatment. Lane 1 represents normal hippocampus, lane 2 the AD model hippocampus, lanes 3, 4, and 5 correspond to increasing doses of QianCengTa compound, and lane 6 is the positive control drug. The Western blot results indicate that QianCengTa compound exhibits a therapeutic effect on AD.

This antibody is highly specific and efficient, suitable for Western blot detection of Anti-PI3K p85 alpha Antibody protein in mouse hippocampus, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU A00318-1
Application Western Blot
Sample Mouse brain tissue
Sample Processing Description Mouse brain tissue was lysed in RIPA buffer supplemented with protease inhibitors at 4°C for 2 hours. The lysate was centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer and heated at 95–100°C for 10 minutes to denature. Then, 15 μL of each protein sample was loaded per lane for electrophoresis.
Other Reagents Blocking buffer
Primary Antibody PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for PIK3R1 and the loading control β-actin in mouse brain tissues from normal mice, model group, and mice treated with high and low doses of drug AB. Although the expression differences between experimental groups are not pronounced, the Western blot results obtained with this antibody are still clear and well-defined.

The antibody has good specificity, with a clear target band at the correct position and minimal non-specific bands.

Excellent, submitted by on
SKU M00566
Application Western Blot
Sample Human A549 cells, HCC1833 cells, LU65 cells, PC-9 cells
Sample Processing Description After normal cell culture, total proteins were extracted using RIPA lysis buffer with protease inhibitors. Protein concentration was determined by BCA assay, and then the four samples were adjusted to a uniform concentration of 3 mg/mL using loading buffer.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The purpose of the experiment is to examine the differences in ADAM10 protein expression among different types of human lung cancer cells, in order to select suitable cells for subsequent experiments.

The antibody shows a clear target band at the correct position, and the results are good.

Excellent, submitted by on
SKU M00297
Application Western Blot
Sample Mouse hippocampal tissue
Sample Processing Description An Alzheimer’s disease (AD) model was induced by intracerebroventricular injection of streptozotocin (STZ) in mice. The mice were subsequently treated with four different doses of Qianlenta Heji, and total protein was extracted from the hippocampal tissues.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary c-FOS serves as a marker of neuronal activation under pathological conditions. Its expression is low in normal brain tissue but elevated in AD model mice, and is reduced after treatment. In the WB assay, lane 1 corresponds to hippocampal tissue from normal mice, lane 2 to AD model mice, lanes 3–5 to hippocampi treated with increasing doses of Qianlenta Heji, and lane 6 to a positive control drug. The results demonstrate that Qianlenta Heji exhibits therapeutic effects in the AD model.

The antibody has high specificity and strong potency, producing excellent results.

Excellent, submitted by on
SKU A00220-4
Application Immunofluorescence
Sample Normal and preterm human placenta
Sample Processing Description Clinically collected term and preterm placentas were processed for paraffin embedding and sectioning in the longitudinal orientation, allowing visualization of both the amnion and chorion.
Other Reagents Goat serum, DAPI (AR1176,Boster Bio),Anti-fade mounting medium
Primary Antibody Cd86 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:500, 1 hour in room temperature
Detection Imaging system:Leica DM2500
Results Summary CD86 is a marker of M1 macrophages. In normal placenta, macrophages are generally absent or very few, and M1 macrophages are even less. In preterm placenta, due to inflammation, the number of macrophages increases and they tend to polarize toward the M1 type. Our experimental results strongly support this theory and are consistent with the expected data.