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A strong nuclear signal for Cyclin E was observed in proliferating cells by IF . Results were reproducible and matched expected patterns.

Excellent, submitted by on
SKU DZ41312
Application Immunofluorescence
Sample Human HaCaT cell
Sample Processing Description Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked in 1% BSA for 1 hour before incubation with the primary antibody.
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:100-1:200, overnight at 4 ℃
Secondary Antibody Goat anti-rabbit IgG conjugated to Alexa Fluor 488
Secondary Incubation 1:1000, 1-2 hours in room temperature
Other Reagents used PBS, 0.1% Triton X-100, 1% BSA, DAPI for nuclear counterstaining
Detection Fluorescence microscopy using Alexa Fluor 488 (excitation 488 nm, emission 519 nm) Using Confocal Microscope Zeiss LSM 900
Results Summary The CyclinE-FL antibody (DZ41312) worked in IF application using human cell lines. In IF, the nuclear localization of Cyclin E matched the known expression pattern with good specificity. A fluorescent secondary antibody (Alexa Fluor 488) was used for detection and gave clear signal. Overall, a reliable antibody for full length Cyclin E detection in human cell systems.

This antibody is highly efficient and specific, suitable for Western blot detection of PTEN protein in rat colon tissue, with only minor nonspecific bands.

Excellent, submitted by on
SKU M00006-2
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody PTEN Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic of Western blot results for the target protein PTEN and the internal control Actin in rat colon across different groups. Expression was increased in the model group. Among the low, medium, and high doses of the herbal treatment, the low-dose group showed the best effect. The target bands are clear, and the experimental results are satisfactory.

A strong nuclear signal for Cyclin E was observed in proliferating cells by IF. Results were reproducible and matched the expected patterns.

Excellent, submitted by on
SKU DZ41311
Application Immunofluorescence
Sample Human HaCaT cell
Sample Processing Description Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked in 1% BSA for 1 hour before incubation with the primary antibody.
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:100-1:200, overnight at 4 ℃
Secondary Antibody Goat anti-rabbit IgG conjugated to Alexa Fluor 488
Secondary Incubation 1:1000, 1-2 hours in room temperature
Detection Fluorescence microscopy using Alexa Fluor 488 (excitation 488 nm, emission 519 nm) Using Confocal Microscope Zeiss LSM 900
Results Summary The CyclinE-D15 antibody (DZ41311) worked well in IF application using human cell lines. In IF, the nuclear localization of Cyclin E matched the known expression pattern. The antibody showed good specificity with a minimal background. A fluorescent secondary antibody (Alexa Fluor 488) was used for detection and gave strong, clear signal. Overall, a reliable antibody for Cyclin E detection in human cell systems.

This antibody is highly efficient and specific, suitable for Western blot detection of MUC6 protein in rat colon tissue, with only minor nonspecific bands.

Excellent, submitted by on
SKU A02143
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Gastric Mucin/MUC6 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows the Western blot results of the target protein MUC6 and the internal control Actin in rat colon tissue across different groups. The target bands are clear and well defined, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of JAK2 protein in rat colon tissue, with only minor nonspecific bands.

Excellent, submitted by on
SKU M00027
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody JAK2 Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows the Western blot results of the target protein JAK2 and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and well defined, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of Claudin 2 protein in rat colon tissue, with only minor nonspecific bands.

Excellent, submitted by on
SKU RP1042
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Cyclin A2/CCNA2 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows the Western blot results of the target protein Claudin 2 and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. Expression of Claudin 2 is elevated in the model group, and among the traditional Chinese medicine groups, the middle-dose group shows the best efficacy. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of CD44 protein in rat colon tissue, with only minor nonspecific bands.

Excellent, submitted by on
SKU A00052
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Cyclin A2/CCNA2 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic of Western blot results for the target protein CCNA2 and the internal control Actin in rat colon across different groups. Expression was increased in the model group. Among the low, medium, and high doses of the herbal treatment, the low-dose group showed the best effect. The target bands are clear, and the experimental results are satisfactory.

This antibody exhibits high efficiency and specificity and is suitable for Western blot detection of CCND1 protein in rat colon tissue, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU M00149-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Cyclin D1 CCND1 Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows the Western blot results of the target protein CCND1 and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for detecting CCNA2 protein in rat colon by Western blot, with only minimal nonspecific bands.

Excellent, submitted by on
SKU PB9424
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Cyclin A2/CCNA2 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic of Western blot results for the target protein CCNA2 and the internal control Actin in rat colon across different groups. Expression was increased in the model group. Among the low, medium, and high doses of the herbal treatment, the low-dose group showed the best effect. The target bands are clear, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for detecting Bcl-XL protein in rat colon by Western blot, with only minimal nonspecific bands.

Excellent, submitted by on
SKU M00181-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Bcl-XL BCL2L1 Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for the target protein Bcl-XL and the internal control Actin in rat colon across the normal rat group, model group, traditional Chinese medicine group, and western medicine group. The target bands are clear and distinct, and the experimental results are satisfactory.