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This antibody is highly specific and efficient, suitable for detecting ATP4B protein in rat colon by Western blot, with only minimal nonspecific bands.

Excellent, submitted by on
SKU A08719-2
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody ATP4B Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for the target protein ATP4B and the loading control Actin in rat colon across the normal group, model group, low-, medium-, and high-dose traditional Chinese medicine groups, and the western medicine group. Expression was increased in the model group, and among the traditional Chinese medicine groups, the high-dose group showed the best efficacy. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for detecting AQP5 protein in rat colon by Western blot, with only minor nonspecific bands.

Excellent, submitted by on
SKU A03085
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Aquaporin 5/AQP5 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, suitable for detecting AQP5 protein in rat colon by Western blot, with only minimal nonspecific bands.

This antibody is highly efficient and specific, suitable for detecting AQP4 protein in rat colon by Western blot, with only minor nonspecific bands.

Excellent, submitted by on
SKU A01049
Application Western Blot
Sample rat colon tissue
Sample Processing Description Samples were lysed in RIPA buffer containing protease inhibitor PMSF (100:1) for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Aquaporin 4/AQP4 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for the target protein AQP4 and the loading control Actin in rat colon across the normal, model, traditional Chinese medicine, and western medicine groups. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for detecting AQP4 protein in rat colon by Western blot, with only minimal nonspecific bands.

Excellent, submitted by on
SKU PB9475
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Aquaporin 4/AQP4 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for the target protein AQP4 and the loading control Actin in rat colon across the normal, model, traditional Chinese medicine, and western medicine groups. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for Western blot detection of AQP3 protein in rat colon, with only minor nonspecific bands.

Excellent, submitted by on
SKU PA1488
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer: cells/tissues were lysed with PMSF protease inhibitor (100:1) for 10 min, followed by centrifugation at 12,000 rpm for 15 min. The supernatant was collected, mixed with 5× loading buffer, denatured at 100 °C for 10 min, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Aquaporin 3/AQP3 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of the target protein AQP3 and the internal control Actin in rat colon across different groups. AQP3 expression was decreased in the model group and increased in all treatment groups, with the Western medicine group （PC）showing higher levels than the traditional Chinese medicine group (TCM). The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for detecting AKT1 protein in rat colon by Western blot, with only minor nonspecific bands.

Excellent, submitted by on
SKU M00024-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer: Add protease inhibitor PMSF (100:1), lyse for 10 min, centrifuge at 12,000 rpm for 15 min, transfer the supernatant and mix with 5× loading buffer, denature at 100 °C for 10 min, and load onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody AKT1 Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:1000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic of Western blot results for the target protein AKT1 and the internal control Actin in rat colon across different groups. No significant differences were observed between groups; the target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for Western blot detection of AKT1 (Phospho-T450) protein in rat colon, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU P00024-2
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer supplemented with PMSF (100:1) was used to lyse the samples for 10 min. The lysates were centrifuged at 12,000 rpm for 15 min, and the supernatants were collected. Fivefold loading buffer was added, and the samples were denatured at 100 °C for 10 min before loading onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Phospho-AKT1 (T450) Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of AKT1 (Phospho-T450) and the internal control Actin in rat colon tissues from different groups. Among the low, medium, and high doses of the traditional Chinese medicine, the high-dose group exhibits the best therapeutic effect. The target bands are clear and well defined, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for Western blot detection of CRCP protein in MCF-7 cells, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU M04247
Application Western Blot
Sample Human mcf-7 cells
Sample Processing Description RIPA lysis buffer supplemented with a protease inhibitor cocktail was added to the cell culture dish, and the cells were lysed on ice for 30 min. The lysates were then centrifuged, and the supernatants were collected. Protein concentration was determined, and samples were adjusted to equal concentrations. Subsequently, 5× protein loading buffer was added, and the samples were denatured at 100 °C for 10 min. A total of 15 μL of protein sample was loaded per lane for SDS-PAGE electrophoresis.
Other Reagents Blocking buffer
Primary Antibody CRCP Rabbit Monoclonal Antibody
Primary Incubation 1:500, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary As shown in the figure, the expression levels of CRCP protein in MCF-7 cells at different drug treatment time points are presented. The Western blot results obtained with this antibody are clear; although there are a few faint nonspecific bands above, they do not affect result interpretation. It can be observed that the expression of the target protein in MCF-7 cells increases with longer drug treatment times.

This antibody is highly specific and efficient, suitable for Western blot detection of STAT3 protein in MCF-7 cells, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU M00007
Application Western Blot
Sample Human mcf-7 cells
Sample Processing Description RIPA lysis buffer supplemented with a protease inhibitor cocktail was added to the cell culture dish and incubated on ice for 30 min. The lysates were then centrifuged, and the supernatants were collected. Protein concentration was determined, and samples were adjusted accordingly. Five times protein loading buffer was added, and the samples were denatured at 100 °C for 10 min. A total of 15 μL of protein sample was loaded per lane for SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody STAT3 Rabbit Monoclonal Antibody
Primary Incubation 1:500, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, suitable for Western blot detection of STAT3 protein in MCF-7 cells, with only minor nonspecific bands observed.

This antibody is highly specific and efficient, suitable for Western blot detection of P2RX7 protein in HT22 cells, producing clear and single bands.

Excellent, submitted by on
SKU A01208-3
Application Western Blot
Sample Mouse HT-22 cells
Sample Processing Description After digestion, cells were collected by centrifugation and lysed in 1 mL of RIPA buffer containing protease inhibitors on ice for 1 h. The lysates were centrifuged, and the supernatants were collected. After protein quantification, 5× loading buffer was added, and the samples were denatured at 100 °C for 10 min before loading onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody P2X7/P2RX7 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The control represents HT22 cells without drug treatment, while Low, Medium, and High correspond to experimental groups treated with three different drug concentrations for 24 h. The Western blot results obtained with this antibody are clear and well-defined, allowing comparison of expression differences between groups. The expression of the target protein slightly increases with increasing drug concentration.