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| SKU | A02285-2 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Mouse skin |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
Source: Biocompare.com
| SKU | DZ33938-1 |
|---|---|
| Application | Western Blot |
| Sample | Insect, D. melanogaster |
| Detection | ECL |
"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."
Source: Biocompare.com
| SKU | DZ33980 |
|---|---|
| Application | Western Blot |
| Sample | Recombinant protein expressed in and purified from E. coli |
| Detection | Chemiluminescence |
"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."
Source: Biocompare.com
| SKU | PB9318 |
|---|---|
| Application | Western Blot |
| Sample | Dorsal root ganglia (neuronal cells) |
| Detection | CyDye |
"We used Anti-PKC Alpha Picoband™ Antibody for immunoprecipitation experiment from neuronal cells in order to characterize PKC Alpha expression in different conditions. Strong positive band with right mass. Works great without optimization! Good quality and ready for publish Ab."
Source: Biocompare.com
| SKU | DZ07988 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos, 5 days old |
| Detection | Confocal Microscopy |
"The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!"
Source: Biocompare.com
| SKU | DZ02095 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 5dpf |
| Detection | Confocal Microscopy |
"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"
Source: Biocompare.com
| SKU | DZ12892 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 24hpf |
| Detection | Confocal Microscopy |
"Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise."
| SKU | A02632-1 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Liver |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C Overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 2ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
| SKU | PB9435 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Rat Brain Tissue |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | 4°C overnight |