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Peer Reviews from Real Labs

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Anti-Smoothened/SMO Antibody for Flow Cytometry

--Yair Fisher, GliXogen Therapeutics, CSO

Excellent, submitted by on

Source: Customer Feedback Submission

SKU A00451-1
Application Flow Cytometry
Sample C3H10T1/2 mouse cell line

"Overlay histogram showing C3H10T1/2 cells stained with A00451-1 (red line). The cells were blocked with 5% normal goat serum, then incubated with rabbit anti-Smoothened/SMO Antibody (A00451-1, 1:50, 2 μg/0.5x10^6 cells) for 30 min at RT. Alexa Fluor® 647 AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) (1:250) was used as a secondary antibody for 30 minutes at RT. Isotype control antibody (blue line) used under the same conditions. Unlabelled sample (yellow line) was also used as a control."

Western blot for Polyclonal Anti-Drosophila melanogaster (Fruit fly) emp Antibody

--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine

Excellent, submitted by on
SKU DZ41152
Application Western blot
Sample Drosophila melanogaster (head and thorax)
Detection Immobilon Forte Western HRP substrate

"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."

Good Zebrafish Mef2 Antibody from Boster Bio

--Jingli Cao, Assistant Professor, Weill Cornell Medical College

Excellent, submitted by on
SKU DZ01398-1
Application Immunofluorescence
Sample Zebrafish heart
Detection Confocal Microscopy

"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."

Good GATA3 Antibody from BosterBio

--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

SKU M00593
Application Western Blot
Sample HEK293T
Detection ECL

"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."

Immunohistochemistry for Anti-TPR Antibody

Excellent, submitted by on
SKU A00695-1
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Mouse Brain
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37 °C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2 ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry review for CD147 Emmprin BSG antibody

Excellent, submitted by on
SKU A00248-1
Application Immunohistochemistry (paraffin embedded)
Blocking step 5% BSA as blocking agent for 30 min at 37°C
Sample Prostate cancer
Fixative Fixed with 4% paraformaldehyde
Primary Incubation 4°C overnight
Primary Incubation diluent 5% BSA in TBS
Primary Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Dilution The kit was ready to use, no dilution needed
Secondary Incubation at 37°C for 30 min

Immunocytochemistry for Anti-E Cadherin 1/CDH1 Antibody

Excellent, submitted by on
PB9561 Immunohistochemistry

The antibody worked perfectly, Great sensitivity

SKU PB9561
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
SampleMouse Liver
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Immunohistochemistry for Anti-CD5 Antibody

Excellent, submitted by on
SKU A00480-2
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Tonsil
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 0.5 ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunocytochemistry for Anti-HnRNP D/AUF1/HNRNPD Antibody

Excellent, submitted by on
SKU A09982-1
Application Immunocytochemistry
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Colorectal tissue
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry Review For Anti-CD147 Emmprin BSG Antibody

Excellent, submitted by on
SKU A00248-1
Application Immunohistochemistry (paraffin embedded)
Blocking step 5% BSA as blocking agent for 30 min at 37°C
Sample Placenta
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min