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Source: Biocompare.com
| SKU | M00593 |
|---|---|
| Application | Western Blot |
| Sample | HEK293T |
| Detection | ECL |
"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."
Source: Biocompare.com
| SKU | EK0422 |
|---|---|
| Application | ELISA |
| Starting Material | Crispr MEK and STAT3 KO CAFs |
| Tips | This kit doesn't provide PC |
"We investigate how alterations in RAS and STAT3 signaling pathways affect the tumor stroma and immune microenvironment in pancreatic cancer. This kit was used to detect the IL-12 level in CM from Crispr MEK and STAT3 KO CAFs. The whole process was about 7 hours including sample preparation."
Source: Biocompare.com
| SKU | EK0434 |
|---|---|
| Application | Testing laminin concentration in hydrogel |
| Starting Material | Hydrogel |
| Tips | Supernatant used for ELISA |
"Positive:(1) Very straight forward and easy to use kits.(2) Accurate results(3) Removable well-strips so experiments can be staggered.Negative:(1) Price point - could be cheaper(2) Reagents have to be used within 1 month upon defrosting."
| SKU | A00695-1 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Mouse Brain |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37 °C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 2 ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
| SKU | A00248-1 |
|---|---|
| Application | Immunohistochemistry (paraffin embedded) |
| Blocking step | 5% BSA as blocking agent for 30 min at 37°C |
| Sample | Prostate cancer |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Incubation | 4°C overnight |
| Primary Incubation diluent | 5% BSA in TBS |
| Primary Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Dilution | The kit was ready to use, no dilution needed |
| Secondary Incubation | at 37°C for 30 min |
The antibody worked perfectly, Great sensitivity
| SKU | PB9561 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Mouse Liver |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 2ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | 4°C overnight |
| SKU | M00063-2 |
|---|---|
| Application | Immunohistochemistry (Paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Oesophagus |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 2ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
| SKU | A00480-2 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Tonsil |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 0.5 ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
| SKU | A09982-1 |
|---|---|
| Application | Immunocytochemistry |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Colorectal tissue |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
| SKU | A00248-1 |
|---|---|
| Application | Immunohistochemistry (paraffin embedded) |
| Blocking step | 5% BSA as blocking agent for 30 min at 37°C |
| Sample | Placenta |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |