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Product Info Summary
| SKU: | M06819-8 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ChIP, ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Acetyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM154
SKU/Catalog Number
M06819-8
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Acetyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM154 (Catalog # M06819-8). Tested in ChIP, WB, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acetyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM154 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-8)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM154
Isotype
Rabbit IgG
Immunogen
An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys36).
Cross-reactivity
This antibody reacts to Histone H3 acetylated at Lysine 36 (K36ac), which is not affected by the phosphorylation of neighboring Ser31. No cross-reactivity with other acetylated Lysines in histone H3.
Reactive Species
M06819-8 is reactive to H3F3A in Human, Vertebrates
Calculated molecular weight
15.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06819-8 is guaranteed for ChIP, ELISA, ICC, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ChIP: 1µg-5 µg
Western Blot: 0.5 ug/mL - 2 ug/mL
ICC: 0.5 ug/mL - 2 ug/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.1 ug/mL – 0.5 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM154 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 36 in treated HeLa.
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Specificity Test result
RM154 specifically reacts to Histone H3 acetylated at Lysine 36 (K36ac), and is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
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ICC result
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys36) Rabbit mAb RM154 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Specific Publications For Anti-Acetyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM154 (M06819-8)
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