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Product Info Summary
| SKU: | PB9973 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Aconitase 2/ACO2 Antibody Picoband®
SKU/Catalog Number
PB9973
PB1026 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aconitase 2/ACO2 Antibody Picoband® catalog # PB9973. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aconitase 2/ACO2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9973)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Aconitase 2, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9973 is reactive to ACO2 in Human, Mouse, Rat
Observed Molecular Weight
85 kDa
Calculated molecular weight
85.4 kDa
Background of ACO2
Aconitase 2, mitochondrial is a protein that in humans is encoded by the ACO2 gene. The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15 (PRSS15), also known as Lon protease, after oxidative modification.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9973 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human U87 whole cell, human Jurkat whole cell, human HepG2 whole cell, rat brain tissue, rat skeletal muscle tissue, mouse brain tissue, mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Western blot analysis of ACO2 using anti-ACO2 antibody (PB9973).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat skeletal muscle tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACO2 antigen affinity purified polyclonal antibody (Catalog # PB9973) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACO2 at approximately 85 kDa. The expected band size for ACO2 is at 85 kDa.
Specific Publications For Anti-Aconitase 2/ACO2 Antibody Picoband® (PB9973)
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5 Customer Q&As for Anti-Aconitase 2/ACO2 Antibody Picoband®
Question
We are currently using anti-Aconitase 2/ACO2 antibody PB9973 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The anti-Aconitase 2/ACO2 antibody (PB9973) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-03
Question
Would PB9973 anti-Aconitase 2/ACO2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
You can see on the product datasheet, PB9973 anti-Aconitase 2/ACO2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-25
Question
Would anti-Aconitase 2/ACO2 antibody PB9973 work on dog WB with cajal-retzius cell fetal brain cortex?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Our lab technicians have not tested anti-Aconitase 2/ACO2 antibody PB9973 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Aconitase 2/ACO2 antibody PB9973 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cajal-retzius cell fetal brain cortex in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Aconitase 2/ACO2 antibody PB9973. Let me know if you need anything else.
E. Parker
Verified customer
Asked: 2014-11-19
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-19
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-Aconitase 2/ACO2 antibody PB9973. Please let me know if you require anything else.
M. Johnson
Verified customer
Asked: 2014-10-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-27
