Anti-Aconitase 2/ACO2 Antibody Picoband™

Aconitase 2 antibody

Boster Bio Anti-Aconitase 2/ACO2 Antibody Picoband™ catalog # PB9973. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9973
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Aconitase 2/ACO2 Antibody Picoband™

View all Aconitase 2 Antibodies

SKU/Catalog Number

PB9973

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aconitase 2/ACO2 Antibody Picoband™ catalog # PB9973. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aconitase 2/ACO2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9973)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Aconitase 2, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9973 is reactive to ACO2 in Human, Mouse, Rat

Applications

PB9973 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

85.425kDa

Background of Aconitase 2

Aconitase 2, mitochondrial is a protein that in humans is encoded by the ACO2 gene. The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15 (PRSS15), also known as Lon protease, after oxidative modification.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For ACO2 (Source: Uniprot.org, NCBI)

Gene Name

ACO2

Full Name

Aconitate hydratase, mitochondrial

Weight

85.425kDa

Superfamily

aconitase/IPM isomerase family

Alternative Names

aconitase 2, mitochondrial; Aconitase; aconitate hydratase, mitochondrial; ACONM; Citrate hydro-lyase; EC 4.2.1; EC 4.2.1.3; MGC20605; MGC33908 ACO2 ACONM, HEL-S-284, ICRD, OCA8, OPA9 aconitase 2 aconitate hydratase, mitochondrial|aconitase 2, mitochondrial|citrate hydro-lyase|epididymis secretory sperm binding protein Li 284|mitochondrial aconitase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACO2, check out the ACO2 Infographic

ACO2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Aconitase 2/ACO2 Antibody Picoband™

Question

We are currently using anti-Aconitase 2/ACO2 antibody PB9973 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

The anti-Aconitase 2/ACO2 antibody (PB9973) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-03

Question

Would PB9973 anti-Aconitase 2/ACO2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

You can see on the product datasheet, PB9973 anti-Aconitase 2/ACO2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-25

Question

Would anti-Aconitase 2/ACO2 antibody PB9973 work on dog WB with cajal-retzius cell fetal brain cortex?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

Our lab technicians have not tested anti-Aconitase 2/ACO2 antibody PB9973 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Aconitase 2/ACO2 antibody PB9973 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cajal-retzius cell fetal brain cortex in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-21

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Aconitase 2/ACO2 antibody PB9973. Let me know if you need anything else.

E. Parker

Verified customer

Asked: 2014-11-19

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-11-19

Question

See below the WB image, lot number and protocol we used for cervix carcinoma using anti-Aconitase 2/ACO2 antibody PB9973. Please let me know if you require anything else.

M. Johnson

Verified customer

Asked: 2014-10-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-27

Size

Conjugation

Total: $370

SKU:PB9973

In stock, 3+ left.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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