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Product Info Summary
| SKU: | M11449 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ACSF2 Mouse Monoclonal Antibody [Clone ID: OTI6E4]
SKU/Catalog Number
M11449
Size
100 µl
Description
Boster Bio ACSF2 mouse monoclonal antibody, clone OTI6E4 (formerly 6E4). Catalog# M11449. Tested in FC, IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ACSF2 Mouse Monoclonal Antibody [Clone ID: OTI6E4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11449)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI6E4
Isotype
IgG1
Immunogen
Full length human recombinant protein of human ACSF2 (NP_079425) produced in HEK293T cell.
Reactive Species
M11449 is reactive to ACSF2 in Human
Calculated molecular weight
68.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11449 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:150
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACSF2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ACSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either ACSF2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACSF2 antibody (M11449)
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Anti-ACSF2 mouse monoclonal antibody (M11449) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACSF2.
Specific Publications For Anti-ACSF2 Mouse Monoclonal Antibody [Clone ID: OTI6E4] (M11449)
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