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Product Info Summary
| SKU: | M07383 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-AK3L1 (AK4) Mouse Monoclonal Antibody [Clone ID: OTI3B1]
SKU/Catalog Number
M07383
Size
100 µl
Description
Boster Bio AK4 mouse monoclonal antibody, clone OTI3B1 (formerly 3B1). Catalog# M07383. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-AK3L1 (AK4) Mouse Monoclonal Antibody [Clone ID: OTI3B1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07383)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3B1
Isotype
IgG1
Immunogen
Full length human recombinant protein of human AK4 (NP_037542) produced in HEK293 cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07383 is reactive to AK4 in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
25.268kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07383 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK4.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-AK4 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK4 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either AK4 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK4 antibody (M07383)
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Anti-AK4 mouse monoclonal antibody (M07383) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK4.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and AK4-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-AK4 monoclonal antibody M07383 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Protein Target Info & Infographic
Gene/Protein Information For AK4 (Source: Uniprot.org, NCBI)
Gene Name
AK4
Full Name
Adenylate kinase 4, mitochondrial
Weight
25.268kDa
Superfamily
adenylate kinase family
Alternative Names
adenylate kinase 3; adenylate kinase 3-like 1; Adenylate kinase 3-like; adenylate kinase 4; AK3adenylate kinase isoenzyme 4, mitochondrial; AK3L1; AK3L2; ATP-AMP transphosphorylase; EC 2.7.4; EC 2.7.4.3; GTP:AMP phosphotransferase; MGC166959; mitochondrial adenylate kinase-3; nucleoside-triphosphate-adenylate kinase AK4 AK 4, AK3, AK3L1, AK3L2 adenylate kinase 4 adenylate kinase 4, mitochondrial|ATP-AMP transphosphorylase|GTP:AMP phosphotransferase AK4, mitochondrial|adenylate kinase 3-like 1|adenylate kinase isoenzyme 4, mitochondrial|epididymis secretory sperm binding protein|mitochondrial adenylate kinase-3|nucleoside-triphosphate-adenylate kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AK4, check out the AK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AK3L1 (AK4) Mouse Monoclonal Antibody [Clone ID: OTI3B1] (M07383)
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