Anti-AKT AKT1 Antibody

Boster Bio Anti-AKT AKT1 Antibody catalog # A00024-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 17 publication(s).

Product Info Summary

SKU: A00024-1
Size: 100ug
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, WB

Product Name

Anti-AKT AKT1 Antibody

See all Akt1 products

SKU/Catalog Number

A00024-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-AKT AKT1 Antibody catalog # A00024-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Cite This Product

Anti-AKT AKT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00024-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Clonality

Polyclonal

Immunogen

Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00024-1 is reactive to AKT1 in Human, Mouse, Rat

Applications

A00024-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

IF Microscopy: 1:500 - 1:2,000
Flow Cytometry: User Optimized
Western Blot: 1:1,000 - 1:5,000
Immunohistochemistry: 1:500 - 1:2,000
Other Dilution: User Optimized

Validation Images & Assay Conditions

Gene/Protein Information For AKT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P31749

Gene ID

207

Gene Name

AKT1

Full Name

RAC-alpha serine/threonine-protein kinase

Weight

55.686kDa

Superfamily

protein kinase superfamily

Alternative Names

AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AKT1, check out the AKT1 Infographic

AKT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AKT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00024-1 has been cited in 17 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xu Y,Chen W,Chen Z,Huang M,Yang F,Zhang Y.Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway.Drug Des Devel Ther.2021 Feb 22;15:753-767.doi:10.2147/DDDT.S280217.PMID:33654381;
Species: Rat
A00024-1 usage in article: APP:WB, SAMPLE:PC12 CELL, DILUTION:1:500

Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
A00024-1 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000

Ji F,Wang Y,Yuan J,Wu Q,Wang J,Liu D.The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells.J Cell Physiol.2020 Apr;235(4):3548-3557.doi:10.1002/jcp.29243.Epub 2019 Sep 30.PMID:31566725.
Species: Rat
A00024-1 usage in article: APP:IF, SAMPLE:ADSCS, DILUTION:1:400

A small interfering RNA targeting osteopontin as gastric cancer therapeutics

Short hairpin RNA targeting AKT1 and PI3K/p85 suppresses the proliferation and self-renewal of lung cancer stem cells

Protective Effects of Sodium (?)-5-Bromo-2-(%u03B1-Hydroxypentyl) Benzoate in a Rodent Model of Global Cerebral Ischemia

Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways

Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice

Progesterone alleviates hypoxic-ischemic brain injury via the Akt/GSK-3? signaling pathway

Li R, Cui K, Liu K, Li H, Zhang Y, Liu X, Chen R, Li M, Wang T, Wang S, Liu J, Rao K. Sci Rep. 2017 Oct 18;7(1):13464. doi: 10.1038/s41598-017-12907-1. Metabolic syndrome in rats is associated with erectile dysfunction by impairing PI3K/Akt/eNOS a...

Wang Q, Sun X, Li X, Dong X, Li P, Zhao L. Mol Med Rep. 2015 Jan;11(1):151-8. Doi: 10.3892/Mmr.2014.2762. Epub 2014 Oct 23. Resveratrol Attenuates Intermittent Hypoxia-Induced Insulin Resistance In Rats: Involvement Of Sirtuin 1 And The Phosphatid...

Wan J, Che Y, Kang N, Wu W. Mol Med Rep. 2015 Jul;12(1):83-92. Doi: 10.3892/Mmr.2015.3368. Epub 2015 Feb 17. Socs3 Blocks Hif-1?? Expression To Inhibit Proliferation And Angiogenesis Of Human Small Cell Lung Cancer By Downregulating Activation Of ...

Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Cardiovasc Diabetol. 2013 Feb 26;12:37. Doi: 10.1186/1475-2840-12-37. Heparanase Induced By Advanced Glycation End Products (Ages) Promotes Macrophage Migration Involving Rage And Pi3K/Akt Pathway.

Wang Gg, Li W, Lu Xh, Zhao X, Xu L. Croat Med J. 2013 Apr;54(2):171-9. Taurine Attenuates Oxidative Stress And Alleviates Cardiac Failure In Type I Diabetic Rats.

Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.

Have you used Anti-AKT AKT1 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-AKT AKT1 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-AKT AKT1 Antibody

Question

Is a blocking peptide available for product anti-AKT antibody (A00024-1)?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

We do provide the blocking peptide for product anti-AKT antibody (A00024-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-27

Question

We want to test anti-AKT antibody A00024-1 on human foreskin for research purposes, then I may be interested in using anti-AKT antibody A00024-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

The products we sell, including anti-AKT antibody A00024-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-26

Question

I was wanting to use your anti-AKT antibody for IHC for human foreskin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human foreskin identification?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

It shows on the product datasheet, A00024-1 anti-AKT antibody has been tested for ELISA, Flow Cytometry, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human foreskin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-21

Question

We have tried in the past anti-AKT antibody for IF on cervix carcinoma in the past. I am using human, and We want to use the antibody for Flow Cytometry next. My lab would like examining cervix carcinoma as well as left adrenal gland in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

I looked at the website and datasheets of our anti-AKT antibody and it seems that A00024-1 has been tested on human in both IF and Flow Cytometry. Thus A00024-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-16

Question

Does anti-AKT antibody A00024-1 work for IHC with foreskin?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

According to the expression profile of foreskin, AKT1 is highly expressed in foreskin. So, it is likely that anti-AKT antibody A00024-1 will work for IHC with foreskin.

Boster Scientific Support

Answered: 2019-10-11

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for foreskin using anti-AKT antibody A00024-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-24

Question

Is this A00024-1 anti-AKT antibody reactive to the isotypes of AKT1?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

The immunogen of A00024-1 anti-AKT antibody is anti-AKT Pan Reactive antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-09

Question

I see that the anti-AKT antibody A00024-1 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-19

Question

We are currently using anti-AKT antibody A00024-1 for rat tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-19

Answer

The anti-AKT antibody (A00024-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-19

Question

Can you help my question with product A00024-1, anti-AKT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

N. Kulkarni

Verified customer

Asked: 2017-09-20

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00024-1 anti-AKT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-09-20

Question

Our team were satisfied with the WB result of your anti-AKT antibody. However we have seen positive staining in foreskin cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?

N. Roberts

Verified customer

Asked: 2017-04-20

Answer

From what I have seen in literature, foreskin does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-04-20

Question

We have seen staining in mouse left adrenal gland. Any tips? Is anti-AKT antibody supposed to stain left adrenal gland positively?

E. Zhang

Verified customer

Asked: 2017-01-06

Answer

Based on literature left adrenal gland does express AKT1. Based on Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-01-06

Question

Does A00024-1 anti-AKT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Wu

Verified customer

Asked: 2016-06-13

Answer

You can see on the product datasheet, A00024-1 anti-AKT antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-06-13

Question

See attached the WB image, lot number and protocol we used for foreskin using anti-AKT antibody A00024-1. Please let me know if you require anything else.

B. Bhatt

Verified customer

Asked: 2015-02-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-02-02

Question

Do you have a BSA free version of anti-AKT antibody A00024-1 available?

D. Bhatt

Verified customer

Asked: 2013-03-05

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-AKT antibody A00024-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-03-05

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00024-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.