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Product Info Summary
| SKU: | A02635-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Alpha Actinin/ACTN1 Antibody
SKU/Catalog Number
A02635-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Alpha Actinin/ACTN1 Antibody catalog # A02635-1. Tested in WB, IHC, IF, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-Alpha Actinin/ACTN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02635-1)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human Alpha Actinin/ACTN1 recombinant protein (Position: Q545-E889).
Reactive Species
A02635-1 is reactive to ACTN1 in Human, Mouse, Rat
Observed Molecular Weight
105 kDa
Calculated molecular weight
103.1 kDa
Background of ACTN1
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02635-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunofluorescence, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
ELISA, 1:100-1000
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, rat heart tissue, rat spleen tissue, rat C6 whole cell, mouse heart tissue, mouse spleen tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of ACTN1 using anti-ACTN1 antibody (A02635-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN1 antigen affinity purified polyclonal antibody (A02635-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ACTN1 at approximately 105 kDa. The expected band size for ACTN1 is at 103 kDa.
Specific Publications For Anti-Alpha Actinin/ACTN1 Antibody (A02635-1)
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