Product Info Summary
SKU: | M03744 |
---|---|
Size: | 100uL |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257
View all Actin Alpha 1 Cardiac Muscle Antibodies
SKU/Catalog Number
M03744
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257 (Catalog # M03744). Tested in IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03744)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM257
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to the N-terminus of human alpha-cardiac actin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
The antibody reacts to human and mouse alpha cardiac actin (Actin, alpha cardiac muscle 1). This antibody may also react to bovine or rat alpha-cardiac actin, as predicted by immunogen homology
Reactive Species
M03744 is reactive to ACTC1 in Human, Mouse
Applications
M03744 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
42.019kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of mouse heart tissue lysate, usingAnti-alpha cardiac actin RM257 at a 1:1000 dilution.
Click image to see more details
Figure 2. Western Blotting result
Western Blot of mouse heart tissue lysate, usingAnti-alpha cardiac actin RM257 at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ACTC1 (Source: Uniprot.org, NCBI)
Gene Name
ACTC1
Full Name
Actin, alpha cardiac muscle 1
Weight
42.019kDa
Superfamily
actin family
Alternative Names
ACTC; ACTC1; actin, alpha, cardiac muscle 1; alpha-Cardiac Actin; ASD5; Cardiac alpha-Actin; CMD1R; CMH11; LVNC4 ACTC1 ACTC, ASD5, CMD1R, CMH11, LVNC4 actin alpha cardiac muscle 1 actin, alpha cardiac muscle 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTC1, check out the ACTC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257 (M03744)
Hello CJ!
M03744 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Photothermal and Enhanced Photocatalytic Therapies Conduce to Synergistic Anticancer Phototherapy with Biodegradable Titanium Diselenide Nanosheets
Heshen Tian,Lei Liu,Ying Wu et al.Resistin-like Molecule β Acts as a Mitogenic Factor in Hypoxic Pulmonary Hypertension via the Ca2+-dependent PI3K/Akt/mTOR and PKC/MAPK Signaling Pathways, 04 December 2020, PREPRINT (Version 3) available at Research Squa
Species: Rat
M03744 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:1:500
Promotion of USP4 to TGF-β1-induced EMT in renal tubular epithelial cells is regulated by Akt through stabilizing TβRI.Jin-Yun Pu,Li-Xia Wang,Jie Wang,Yu Zhang,Jian-Hua Zhou bioRxiv 2020.10.28.358796;doi:https ://doi.org/10.1101/2020.10.28.358796
Species: Rat
M03744 usage in article: APP:WB, SAMPLE:NRK-52E CELLS, DILUTION:NA
Rosiglitazone Ameliorates Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats by Regulating the Extracellular Matrix Remodelling. Xiaofang Wang,Yunjian Zhang,J. Guo, J. Shi
Species: Rat
M03744 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA
Liu J,Zhao B,Zhu H,Pan Q,Cai M,Bai X,Li X,Hu X,Zhang M,Shi J,Zheng Z,Yang A,Hu D.Wnt4 negatively regulates the TGF-β1-induced human dermal fibroblast-to-myofibroblast transition via targeting Smad3 and ERK.Cell Tissue Res.2020 Mar;379(3):537-548.doi:10.10
Species: Human
M03744 usage in article: APP:WB, SAMPLE:FIBROBLAST, DILUTION:1:400
Xiao L,Zhang H,Yang X,Mahati S,Wu G,Xiaheding Y,Bao YX,Xiao H.Role of phosphatidylinositol 3-kinase signaling pathway in radiation-induced liver injury.Kaohsiung J Med Sci.2020 Jul 30.doi:10.1002/kjm2.12279. Epub ahead of print.PMID:32729224.
Species: Rat
Zhao Y, Wang X, Yang S, Song X, Sun N, Chen C, Zhang Y, Yao D, Huang J, Wang J, Zhang Y, Yang B. Kanglexin accelerates diabetic wound healing by promoting angiogenesis via FGFR1/ERK signaling. Biomed Pharmacother. 2020 Oct 28;132:110933. doi: 10.1016/j.bi
Species: Mouse
M03744 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257
Question
We are currently using anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 (M03744) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
Here is the WB image, lot number and protocol we used for muscle using anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
I was wanting to use your anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 for WB for human muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human muscle identification?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
You can see on the product datasheet, M03744 anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-16
Question
Will anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 work on feline WB with myocardium?
C. Miller
Verified customer
Asked: 2018-03-02
Answer
Our lab technicians have not validated anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline myocardium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-02
Question
Is a blocking peptide available for product anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 (M03744)?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
We do provide the blocking peptide for product anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 (M03744). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-08
Question
I would like to test anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 on human muscle for research purposes, then I may be interested in using anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
The products we sell, including anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-20
Question
Is this M03744 anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 reactive to the isotypes of ACTC1?
L. Dhar
Verified customer
Asked: 2014-01-07
Answer
The immunogen of M03744 anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 is A synthetic peptide corresponding to the N-terminus of human alpha-cardiac actin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-01-07