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Product Info Summary
| SKU: | M03744 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257
SKU/Catalog Number
M03744
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257 (Catalog # M03744). Tested in IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03744)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM257
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to the N-terminus of human alpha-cardiac actin
Cross-reactivity
The antibody reacts to human and mouse alpha cardiac actin (Actin, alpha cardiac muscle 1). This antibody may also react to bovine or rat alpha-cardiac actin, as predicted by immunogen homology
Reactive Species
M03744 is reactive to ACTC1 in Human, Mouse
Calculated molecular weight
42.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03744 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of mouse heart tissue lysate, usingAnti-alpha cardiac actin RM257 at a 1:1000 dilution.
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Western Blotting result
Western Blot of mouse heart tissue lysate, usingAnti-alpha cardiac actin RM257 at a 1:1000 dilution.
Specific Publications For Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257 (M03744)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257
Question
We are currently using anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 (M03744) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
Here is the WB image, lot number and protocol we used for muscle using anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
I was wanting to use your anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 for WB for human muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human muscle identification?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
You can see on the product datasheet, M03744 anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-16
Question
Will anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 work on feline WB with myocardium?
C. Miller
Verified customer
Asked: 2018-03-02
Answer
Our lab technicians have not validated anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline myocardium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-02
Question
Is a blocking peptide available for product anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 (M03744)?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
We do provide the blocking peptide for product anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 (M03744). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-11-08
Question
I would like to test anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 on human muscle for research purposes, then I may be interested in using anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
The products we sell, including anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 M03744, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-20
Question
Is this M03744 anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 reactive to the isotypes of ACTC1?
L. Dhar
Verified customer
Asked: 2014-01-07
Answer
The immunogen of M03744 anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal antibody, Clone#RM257 is A synthetic peptide corresponding to the N-terminus of human alpha-cardiac actin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-01-07
