Overview
| Product Name |
Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody
See more alpha Tubulin products |
| Catalog# |
M03989 |
| Pack Size |
0.1 mg (C100) |
| Storage & Handling |
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. |
| Description |
Boster Bio Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody catalog # M03989. Tested in IP, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Pig, Turkey, Yeast. Supplied as 0.1 mg (C100) in Liquid form antibody. |
| Cite This Product |
Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03989)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Mouse |
| Reactive Species |
Human, Mouse, Pig, Turkey, Yeast |
| Applications |
IP, IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. |
Gene/Protein Basic Information For TUBA1A (Source: Uniprot.org, NCBI)
| Uniprot Id | Q71U36 |
|---|
| NCBI Gene Id | 7846 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | TUBA1A |
|---|
| Protein Name | Tubulin alpha-1A chain |
|---|
| Superfamily | tubulin family |
|---|
| Alternative Names | alpha Tubulin|alpha tubulin loading control; alpha tubulin monoclonal; alpha Tubulin; Alpha-Tubulin 3; B-ALPHA-1; DM1A tubulin; dm1a monoclonal; DM1a tubulin; DM1A; FLJ25113; hum-a-tub1; hum-a-tub2; LIS3; TUBA1 monoclonal; TUBA1A; TUBA3; tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B-Alpha-1; tubulin monoclonal; tubulin, alpha 1a; tubulin, alpha 3; tubulin, alpha, brain-specific |
|---|
| Molecular Weight | 50136 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TUBA1A, check out the TUBA1A Infographic
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Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for TUBA1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the TUBA1A infographic
Figure 1. Immunocytochemistry staining of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunocytochemistry staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green) and anti-Vimentin (VI-01; cat. no. 11-254-C100; red). Nucleus is stained with DAPI (blue).
Figure 2. Immunocytochemistry staining of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01; red). Nucleus is stained with DAPI (blue).
Figure 3. Immunocytochemistry staining of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunocytochemistry staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green). Nucleus is stained with DAPI (blue).
Figure 4. Immunohistochemistry validation of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunohistochemistry staining of human heart (paraffin sections) using anti-alpha-tubulin (TU-01).
Commercially tested by LifeSpan BioSciences.
For more protocol information of IHC
Figure 5. IP validation of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa.
L = lysate
IPr = immunoprecipitate (reducing conditions)
IPn = immunoprecipitate (non-reducing conditions)
Figure 6. Western blotting validation for Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody M03989
Use of anti-alpha-tubulin antibody TU-01 as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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