Product Info Summary
SKU: | M14347 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ANKRD53 Mouse Monoclonal Antibody [Clone ID: OTI1E1]
SKU/Catalog Number
M14347
Size
100 µl
Description
Boster Bio ANKRD53 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1). Catalog# M14347. Tested in FC, IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ANKRD53 Mouse Monoclonal Antibody [Clone ID: OTI1E1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14347)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1E1
Isotype
IgG2b
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-300 of human ANKRD53 (NP_079209) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M14347 is reactive to ANKRD53 in Human
Reconstitution
Calculated molecular weight
56.738kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14347 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANKRD53 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ANKRD53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ANKRD53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either ANKRD53 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANKRD53 antibody (M14347)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-ANKRD53 mouse monoclonal antibody (M14347) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD53.
Protein Target Info & Infographic
Gene/Protein Information For ANKRD53 (Source: Uniprot.org, NCBI)
Gene Name
ANKRD53
Full Name
Ankyrin repeat domain-containing protein 53
Weight
56.738kDa
Alternative Names
ankyrin repeat domain 53; ankyrin repeat domain-containing protein 53; FLJ12056; FLJ36160
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANKRD53, check out the ANKRD53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANKRD53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ANKRD53 Mouse Monoclonal Antibody [Clone ID: OTI1E1] (M14347)
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