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- Table of Contents
ANXA5 antibody (PB9044) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of 293T cells using anti-Annexin V/ANXA5 antibody (PB9044).
Overlay histogram showing 293T cells stained with PB9044 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin V/ANXA5 Antibody (PB9044, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing 293T cells stained with PB9044 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin V/ANXA5 Antibody (PB9044, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Annexin V/ANXA5 Antibody Picoband®
ANXA5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ANXA5 antibody was positively detected by WB in:
human Hela whole cell, human K562 whole cell, human 293T whole cell, human HepG2 whole cell, rat heart tissue, mouse heart tissue, mouse RAW264.7 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ANXA5 antibody was positively detected by IHC in:
human colon cancer tissu, mouse kidney tissue, rat kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ANXA5 antibody was positively detected by ICC/IF in:
U2OS cell
ANXA5 antibody was positively detected by FC in:
293T cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Annexin V/ANXA5 Antibody Picoband®
View all ANXA5 Antibodies
View all ANXA5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
35.9 kDa
Background of ANXA5
Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Cite This Product
Anti-Annexin V/ANXA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9044)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
98%
High Homology
Sheep
96%
High Homology
Rabbit
93%
High Homology
Mouse
93%
High Homology
Rat
92%
High Homology
Cow
89%
Moderate Homology
Zebrafish
64%
Low homology
Drosophila
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ANXA5 antibody
Immunohistochemistry (IHC) protocol for ANXA5 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ANXA5 antibody
Flow Cytometry (FC) protocol for ANXA5 antibody
Publications
Application:
Species:
Morphological and Proteomic Analyses Reveal that Unsaturated Guluronate Oligosaccharide Modulates Multiple Functional Pathways in Murine Macrophage RAW264.7 Cells
Published: 2015-03-30
Journal: Marine Drugs
Application: WB
Impact Factor: 2.85
Species: Mouse
PMID: 25830683
Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma,alone and/or synergistically
Published: 2012-10-09
Journal: Oncology Letters
Application: IHC
Impact Factor: 0.11
Species: Human
PMID: 23255903
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Product Promise
We promise Anti-Annexin V/ANXA5 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PB9044
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026