|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-AP2 Alpha Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Transcription factor AP-2-alpha(TFAP2A) detection. Tested with WB in Human;Rat.|
|Cite This Product||Anti-AP2 Alpha Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9118)|
|Replacement Item||This antibody may replace the following items: sc-12726|sc-12726-X|sc-184|sc-184-X|sc-25343|sc-25343-X|sc-53163|sc-53164|sc-55485|sc-6312|sc-6312-X|sc-70361|sc-8975|sc-8975-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human AP2 alpha recombinant protein (Position: M1-G166). Human AP2 alpha shares 98% amino acid (aa) sequence identity with both mouse and rat AP2 alpha.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Transcription factor AP-2-alpha|
|Molecular Weight||48062 MW|
|Protein Function||Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. .|
|Sequence Similarities||Belongs to the AP-2 family.|
|Subcellular Localization||Nucleus .|
|Alternative Names||Transcription factor AP-2-alpha;AP2-alpha;AP-2 transcription factor;Activating enhancer-binding protein 2-alpha;Activator protein 2;AP-2;TFAP2A;AP2TF, TFAP2;|
|Research Areas|||epigenetics and nuclear signaling|transcription|other factors| epigenetics and nuclear signaling|chip'ing antibodies||
Background for Transcription factor AP-2-alpha
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-AP2 Alpha Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-AP2 Alpha Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti AP2A (PB9118) at 0.5ug/ml
WB: Recombinant Human AP2A Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti AP2A (PB9118) at 0.5ug/ml
WB: Rat Spleen Tissue Lysate at 50ug
Predicted bind size: 48KD
Observed bind size: 48KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,