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Product Info Summary
| SKU: | A30987 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Goat |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-AP2A ap2a1-1 Antibody
SKU/Catalog Number
A30987
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-AP2A ap2a1-1 Antibody (Catalog # A30987). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-AP2A ap2a1-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30987)
Host
Goat
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of Human AP2A1 and AP2A2 proteins.
Reactive Species
A30987 is reactive to AP2A in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
107.5 kDa
Background of AP2A
The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrin-coated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly protein complex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit. The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30987 is guaranteed for ELISA, IP, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| ELISA: 1:5 | 000 - 1:20 | 000 |
| WB: 1:500 - 1:2 | 000 |
Validation Images & Assay Conditions
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Western blot using Boster's Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1. Lane 1: HeLa nuclear extract , Lane 2: HeLa , Lane 3: 293 (W09-000-365), Lane 4: A431 , and Lane 5: Jurkat whole cell lysate . In lanes 6-10 the antibody was preincubated with 1 µg/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.
Specific Publications For Anti-AP2A ap2a1-1 Antibody (A30987)
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