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Product Info Summary
| SKU: | PA1442 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-7(P11)/CASP7 Antibody Picoband®
SKU/Catalog Number
PA1442
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-7 (P11)/CASP7 Antibody catalog # PA1442. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-7(P11)/CASP7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1442)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11), different from the related mouse and rat sequences by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1442 is reactive to CASP7 in Human, Mouse, Rat
Observed Molecular Weight
34 kDa
Calculated molecular weight
34.3 kDa
Background of CASP7
CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1442 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: HELA Whole Cell, MCF-7 Whole Cell, Rat Liver Tissue, Rat Kidney Tissue
IHC: Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
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Anti-Caspase-7(P11) antibody, PA1442, Western blotting
All lanes: Anti CASP7(p11) (PA1442) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 34KD
Observed bind size: 34KD
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Anti-Caspase-7(P11) antibody, PA1442, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Specific Publications For Anti-Caspase-7(P11)/CASP7 Antibody Picoband® (PA1442)
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Customer Q&As
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6 Customer Q&As for Anti-Caspase-7(P11)/CASP7 Antibody Picoband®
Question
Will PA1442 anti-Caspase-7(P11)/CASP7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
As indicated on the product datasheet, PA1442 anti-Caspase-7(P11)/CASP7 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-24
Question
I have attached the WB image, lot number and protocol we used for t-cell using anti-Caspase-7(P11)/CASP7 antibody PA1442. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Caspase-7(P11)/CASP7 antibody PA1442. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-12
Question
Is this PA1442 anti-Caspase-7(P11)/CASP7 antibody reactive to the isotypes of CASP7?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
The immunogen of PA1442 anti-Caspase-7(P11)/CASP7 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11)(237-255aa RGSWFVQALCSILEEHGKD), different from the related mouse and rat sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-02
Question
I am interested in to test anti-Caspase-7(P11)/CASP7 antibody PA1442 on rat t-cell for research purposes, then I may be interested in using anti-Caspase-7(P11)/CASP7 antibody PA1442 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Edwards
Verified customer
Asked: 2017-03-21
Answer
The products we sell, including anti-Caspase-7(P11)/CASP7 antibody PA1442, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-03-21
Question
Can you help my question with product PA1442, anti-Caspase-7(P11)/CASP7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Jackson
Verified customer
Asked: 2016-02-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1442 anti-Caspase-7(P11)/CASP7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-02-01
