Anti-ARA9/AIP Antibody Picoband™

Product Name

Anti-ARA9/AIP Antibody Picoband™

View all AIP/ARA9 Antibodies

SKU/Catalog Number

PB9042

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ARA9/AIP Antibody Picoband™ catalog # PB9042. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ARA9/AIP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9042)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ARA9 recombinant protein (Position: D91-H330). Human ARA9 shares 95% amino acid (aa) sequence identity with both mouse and rat ARA9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9042 is reactive to AIP in Human

Applications

PB9042 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

37.636kDa

Background of AIP/ARA9

AIP, also known as, ARA9 or XAP-2, is a protein that in humans is encoded by the AIP gene. This gene is mapped to 11q13.2. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. AIP may play a positive role in aryl hydrocarbon receptor-mediated signalling possibly by influencing its receptivity for ligand and/or its nuclear targeting. It has been shown that AIP is the cellular negative regulator of the hepatitis B virus (HBV) X protein. AIP mutations may be the cause of a familial form of acromegaly, familial isolated pituitary adenoma (FIPA).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ARA9 using anti-ARA9 antibody (PB9042).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate,
Lane 2: COLO320 Whole Cell Lysate,
Lane 3: HT1080 Whole Cell Lysate,
Lane 4: MCF-7 Whole Cell Lysate,
Lane 5: SW620 Whole Cell Lysate,
Lane 6: U87 Whole Cell Lysate,
Lane 7: MM231 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARA9 antigen affinity purified polyclonal antibody (Catalog # PB9042) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARA9 at approximately 38KD. The expected band size for ARA9 is at 38KD.

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Figure 2. IF analysis of ARA9 using anti-ARA9 antibody (PB9042).
ARA9 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ARA9 Antibody (PB9042) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of A431 cells using anti-ARA9 antibody (PB9042).
Overlay histogram showing A431 cells stained with PB9042 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARA9 Antibody (PB9042,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For AIP (Source: Uniprot.org, NCBI)

Alternative Names

ARA9; FKBP16; FKBP37; SMTPHN; XAP2; XAP-2 AIP ARA9, FKBP16, FKBP37, PITA1, SMTPHN, XAP-2, XAP2 aryl hydrocarbon receptor interacting protein AH receptor-interacting protein|Ah receptor activated 9|FK506-binding protein 37|FKBP prolyl isomerase 16|HBV X-associated protein 2|X-associated protein-2|aryl hydrocarbon receptor-associated protein 9|hepatitis B virus X-associated cellular protein 2|immunophilin homolog ARA9

For more info on AIP, check out the AIP Infographic

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In this infographic, you will see the following information for AIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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